リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

大学・研究所にある論文を検索できる 「Identification of a 17 kDa protein that is a potentially novel antigen of lettuce-associated respiratory allergy in farmers」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

コピーが完了しました

URLをコピーしました

論文の公開元へ論文の公開元へ
書き出し

Identification of a 17 kDa protein that is a potentially novel antigen of lettuce-associated respiratory allergy in farmers

Yoshioka, Junya Nagano, Tatsuya Sekiya, Reina Yano, Erika Hatano, Naoya Katsurada, Naoko Yamamoto, Masatsugu Tachihara, Motoko Uno, Yuichi Moriyama, Tatsuya Nishimura, Yoshihiro Kobayashi, Kazuyuki 神戸大学

2023.11

概要

Background: We have identified and reported a novel antigen, nonprotein-specific secreted EP1-like glycoprotein (51 kDa), for lettuce-related respiratory allergy. Objective: We aimed to identify a novel antigen for lettuce-related respiratory allergy that is different from epidermis-specific secreted EP1-like glycoprotein. Methods: Immunoblotting was performed using an immunoglobulin E-specific antibody. The antigen-antibody reaction was confirmed by means of enzyme-linked immunosorbent assaying. LC‒MS/MS analysis was carried out to detect a novel protein found in sera from 3 of 13 patients with lettuce-related respiratory allergy. Finally, we purified a novel protein from Escherichia coli. Results: mmunoblotting assays showed common bands of 17 kDa in the sera of 3 of 13 patients. An enzyme-linked immunosorbent assay confirmed that the patient sera reacted with lettuce latex juice. A 17 kDa protein band that showed antigenic reactivity in 3 of 13 patient sera was identified as a kirola-like protein by LC‒MS/MS. In addition, although we purified this protein, we failed to show the inhibitory effect. Conclusion: A 17 kDa protein that is a potentially novel antigen of lettuce-associated respiratory allergy was identified. In further studies, we will focus on purifying this novel protein to diagnose lettuce allergy.

この論文で使われている画像

参考文献

1. Sekiya R, Nagano T, Moriyama T, et al. Occupational

respiratory allergy to lettuce in lettuce farmers. Clinical &

Experimental Allergy. 2020;50(8):932‐941.

2. San Miguel‐Moncín M, Krail M, Scheurer S, et al. Lettuce

anaphylaxis: identification of a lipid transfer protein as the

major allergen. Allergy. 2003;58(6):511‐517.

3. Bascones O, Rodríguez‐Pérez R, Juste S, Moneo I,

Caballero ML. Lettuce‐induced anaphylaxis. identification of

the allergen involved. J Investig Allergol Clin Immunol.

2009;19(2):154‐157.

4. Vila L, Sánchez G, Sanz ML, et al. Study of a case of

hypersensitivity to lettuce (Lactuca sativa). Clinical &

Experimental Allergy. 1998;28(8):1031‐1035.

ET AL.

5. Alonso MD, Martin JA, Cuevas M, et al. Occupational protein

contact dermatitis from lettuce. Contact Dermatitis. 1993;29(2):

109‐110.

6. Franck P, Kanny G, Dousset B, Nabet P, Moneret‐Vautrin DA.

Lettuce allergy. Allergy. 2000;55(2):201‐202.

7. Paulsen E, Andersen KE. Lettuce contact allergy. Contact

Dermatitis. 2016;74(2):67‐75.

8. Hartz C, Del mar san miguel‐Moncín M, Cisteró‐Bahíma A, et al.

Molecular characterisation of lac s 1, the major allergen from

lettuce (Lactuca sativa). Mol Immunol. 2007;44(11):2820‐2830.

9. Muñoz‐García E, Luengo‐Sánchez O, Haroun‐Díaz E, et al.

Identification of thaumatin‐like protein and aspartyl protease

as new major allergens in lettuce (Lactuca sativa). Mol Nutr

Food Res. 2013;57(12):2245‐2252.

10. Laemmli UK. Cleavage of structural proteins during the assembly

of the head of bacteriophage T4. Nature. 1970;227(5259):680‐685.

11. Kyhse‐Andersen J. Electroblotting of multiple gels: a simple

apparatus without buffer tank for rapid transfer of proteins

from polycrylamide to nitrocellulose. J Biochem Biophys

Methods. 1984;10(3‐4):203‐209.

12. Moriyama T, Yano E, Kitta K, Kawamoto S, Kawamura Y,

Todoriki S. Effect of gamma irradiation on soybean allergen

levels. Biosci Biotechnol Biochem. 2013;77(12):2371‐2377.

13. Ciardiello MA, Giangrieco I, Tuppo L, et al. Influence of the

natural ripening stage, cold storage, and ethylene treatment

on the protein and IgE‐binding profiles of green and gold kiwi

fruit extracts. J Agricult Food Chem. 2009;57(4):1565‐1571.

14. Chruszcz M, Ciardiello MA, Osinski T, et al. Structural and

bioinformatic analysis of the kiwifruit allergen Act d 11, a

member of the family of ripening‐related proteins. Mol

Immunol. 2013;56(4):794‐803.

15. D'Avino R, Bernardi ML, Wallner M, et al. Kiwifruit Act d 11

is the first member of the ripening‐related protein family

identified as an allergen. Allergy. 2011;66(7):870‐877.

SU PPOR T ING INF OR MATI ON

Additional supporting information can be found online

in the Supporting Information section at the end of this

article.

How to cite this article: Yoshioka J, Nagano T,

Sekiya R, et al. Identification of a 17 kDa protein

that is a potentially novel antigen of lettuce‐

associated respiratory allergy in farmers. Immun

Inflamm Dis. 2023;11:e1093. doi:10.1002/iid3.1093

20504527, 2023, 11, Downloaded from https://onlinelibrary.wiley.com/doi/10.1002/iid3.1093 by Kobe University, Wiley Online Library on [29/11/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License

6 of 6

...

参考文献をもっと見る

全国の大学の
卒論・修論・学位論文

一発検索!

この論文の関連論文を見る