Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids

Bellanne-Chantelot, C., Chauveau, D., Gautier, J.F., Dubois-Laforgue, D., Clauin, S., Beaufils, S., Wilhelm, J.M., Boitard, C., Noel, L.H., Velho, G., et al. (2004). Clinical spectrum associated with hepatocyte nuclear factor-1beta mutations. Ann. Intern. Med. 140, 510–517.

Costantini, F., and Kopan, R. (2010). Patterning a complex organ: branching morphogenesis and nephron segmentation in kidney development. Dev. Cell 18, 698–712. https://doi.org/10.1016/j. devcel.2010.04.008.

Desgrange, A., Heliot, C., Skovorodkin, I., Akram, S.U., Heikkila¨ , J., Ronkainen, V.P., Miinalainen, I., Vainio, S.J., and Cereghini, S. (2017). HNF1B controls epithelial organization and cell polarity during ureteric bud branching and collecting duct morphogenesis. Development 144, 4704–4719. https://doi.org/10.1242/dev.154336.

Heidet, L., Decramer, S., Pawtowski, A., Morinie` re, V., Bandin, F., Knebelmann, B., Lebre, A.S., Faguer, S., Guigonis, V., Antignac, C., and Salomon, R. (2010). Spectrum of HNF1B mutations in a large cohort of patients who harbor renal diseases. Clin.

J. Am. Soc. Nephrol. 5, 1079–1090. https://doi.org/ 10.2215/cjn.06810909.

Lokmane, L., Heliot, C., Garcia-Villalba, P., Fabre, M., and Cereghini, S. (2010). vHNF1 functions in distinct regulatory circuits to control ureteric bud branching and early nephrogenesis. Development 137, 347–357. https://doi.org/10.1242/dev.042226.

Mae, S.I., Ryosaka, M., Sakamoto, S., Matsuse, K., Nozaki, A., Igami, M., Kabai, R., Watanabe, A., and Osafune, K. (2020). Expansion of human iPSC- derived ureteric bud organoids with repeated branching potential. Cell Rep. 32, 107963. https:// doi.org/10.1016/j.celrep.2020.107963.

Mae, S.I., Ryosaka, M., Toyoda, T., Matsuse, K., Oshima, Y., Tsujimoto, H., Okumura, s., Shibasaki, A., and Osafune, K. (2018). Generation of branching ureteric bud tissues from human pluripotent stem cells. Biochem Biophys Res Commun 495 (1), 954–961, In press. https://doi.org/10.1016/j.bbrc. 2017.11.105.

Miyazaki, T., Isobe, T., Nakatsuji, N., and Suemori, H. (2017). Efficient adhesion culture of human pluripotent stem cells using laminin fragments in an uncoated manner. Sci. Rep. 7, 41165. https://doi. org/10.1038/srep41165.

Nakayama, M., Nozu, K., Goto, Y., Kamei, K., Ito, S., Sato, H., Emi, M., Nakanishi, K., Tsuchiya, S., and Iijima, K. (2010). HNF1B alterations associated with congenital anomalies of the kidney and urinary tract. Pediatr. Nephrol. 25, 1073–1079. https://doi. org/10.1007/s00467-010-1454-9.

Osathanondh, V., and Potter, E.L. (1963). Development of human kidney as shown by microdissection. II. Renal pelvis, calyces, and papillae. Arch. Pathol. 76, 277–289.

Potter, E.L. (1972). Normal and Abnormal Development of the Kidney (Year Book Medical Publishers).

Wang, P., Chen, Y., Yong, J., Cui, Y., Wang, R., Wen, L., Qiao, J., and Tang, F. (2018). Dissecting the global dynamic molecular profiles of human fetal kidney development by single-cell RNA sequencing. Cell Rep. 24, 3554–3567.e3. https:// doi.org/10.1016/j.celrep.2018.08.056.