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Fig. 1
Immunohistochemical staining for glial cell-line derived neurotrophic factor (GDNF), GDNF
yr
r(GFR)a
la
dr
dd
gt
n(RET) receptors in cultured
human anulus fibrosus (AF) and nucleus pulposus (NP) cells. AF: A-C, NP: D-F; isotype
controls: G. Samples were imaged using confocal microscopy. Nuclei are stained with
mi
e(
.S
eb
:20μm.
GDNF
GFRa1
RET
AF
NP
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Fig. 2
Western blots for glial cell-line derived neurotrophic factor (GDNF)(A), GDNF family
r(GFR)a
l(B) and rearranged during transfection (RET) (C) receptor in cultured
human anulus fibrosus (AF) and nucleus pulposus (NP) cells. A single band directed against
ha
ywasd
.~ -actin (D) served as a loading control.
AF
A:GDNF
38kDa
:GFRa1
67kDa
C:RET
80kDa ~
:1
42kDa
NP
Fig. 3
Detection of mRNA expression by quantitative real-time polymerase chain reaction (PCR) of
glial cell-line derived neurotrophic factor (GDNF) (A), GDNF family receptor (GFR)a
l(B)
and rearranged during transfection (RET) receptor (C) in human annulus fibrosus (AF) and
nucleus pulposus (NP) cells.
r.
A:GDNF
B:GFRa1
C:RET
54
0庄
UOI
SS9JdX0 3 A-1B -
AF
NP
AF
NP
AF
NP
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Fig. 4
Effect of interleukin-1 beta (IL- 1~) onmRNAl
so
fg
lc
l-line derived neurotrophic
factor (GDNF) (A), GDNF fami
yr
r(GFR)a
l(B) and rearranged during transfection
(RET) receptor C) by human anulus fibrosus (AF) and nucleus pulposus (NP) cells. IL- 1~:
Interleukin- 1~'Cont.: C
lg
,*
,v
.c
A:GDNF
C:RET
:GFRa1
098765432
AF
NP
NP
AF
AF
NP
- ︳
aa>︱
U OI
SS<ld
﹂x
8江
柑︳
~,.
~も 伶
IL-1 ~(ng/ml)
~ ~ザ
IL-1~(ng/ml}
i →企•
IL-1~(ng/ml)
~ ~
IL-1~(ng/ml)
" ヽ、もヽ (ヽ・
IL-1~(ng/ml)
IL-1~(ng/ml)
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Fig. 5
Immunohistochemical staining for glial cell-line derived neurotrophic factor (GDNF) (A, D,
,J
,GDNFf
yr
r(GFR)a
l(
,E
,H,K)andr
dd
gt
(RET) receptor (C, F, I, L) in human intervertebral disc (IVD) tissues (anulus fibrosus: AF
[A-F]) and nucleus pulposus: NP [G-L]) at early and advanced stages of degeneration. Scale
:20μm.
GDNF
C ・
RET
“一
IA
aBe1s
GFRa1
GDNF
IG
GFRa1
a6eJs
paoueAPV
RET
ヽ鯵
a6eJs
p gueAP'1
--
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Fig. 6
Percentage of immunopositive cells for glial cell-line derived neurotrophic factor (GDNF)
,GDNFf
yr
r(GFR)a
l(
)andr
dd
gt
n(RET) receptor
(C) in the human IVD tissues of early and advanced stages of disc degeneration. anulus
fibrosus: AF, nucleus pulposus: NP. *P<0.05, **P<0.01 between early and advanced stages
of degeneration.
A:GDNF
:GFRa1
C:RET
90
80
bls e
!l!
i 9A
O¥
SOd'oun
■p
e1
■p
e2
60
50
EE
pe::,ueAp"I
k-﹂
AF
pe:JueAp y
em
k-﹂
NP
Pの:JU8AP V
mm
k-﹂
AF
pe::iue、Ap~
A-﹂
NP
p9::lU8AP"i
k-﹂
AF
p9:,ueApy
A-﹂
NP
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