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Modification of semisolid medium for stab culture to isolate Salmonella enterica from rectal swabs

Fujihara, Masatoshi Sakazume, Noriko Tagaino, Yuuka 北海道大学

2022.02

概要

Semisolid agar has shown good performance for selective isolation of Salmonella enterica. To control the occurrence salmonellosis within a herd, identification of asymptomatic carriers is necessary; however, rectal swab samples are not suitable for inoculation onto semisolid agar. In this study, we lowered the agar concentration of modified semisolid Rappaport agar for stab culture. S. enterica exhibited growth across the entirely of the medium. Other bacteria in bovine fecal samples were not observed to migrate, and the sensitivity for S. enterica isolation was greater than that of commercially available Hajna tetrathionate broth. As inoculation onto isolation agar plates is not necessary if no migration is observed, this stab culture method would drastically simplify the process of S. enterica isolation.

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参考文献

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