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大学・研究所にある論文を検索できる 「Existence of hepatitis B virus surface protein mutations and other variants: demand for hepatitis B infection control in Cambodia」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

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Existence of hepatitis B virus surface protein mutations and other variants: demand for hepatitis B infection control in Cambodia

Ko Ko 広島大学

2020.09.18

概要

Background: This study aimed to detect Hepatitis B virus (HBV) genome sequences and their variants as of
nationwide scale using dried blood spot (DBS) samples and to provide up-to-date reference data for infection
control and surveillance in Cambodia.
Method: Among 2518 children age 5–7 years and their 2023 mothers participated in 2017 Cambodia nationwide
sero-survey on hepatitis B surface antigen (HBsAg) prevalence using multistage random sampling strategy, 95
mothers and 13 children positive to HBsAg were included in this study. HBV DNA was extracted from DBS, then
performed polymerase chain reaction. HBV genotypes and potential variants were examined by partial and full
length genomic analysis.
Results: HBsAg positive rate was 4.7% (95/2023) in mothers and 0.52% (13/2518) in their children. Genotype C
(80.49%) was abundantly found throughout the whole Cambodia whilst genotype B (19.51%) was exclusively found
in regions bordering Vietnam. S gene mutants of HBV were found in 24.29% of mothers and 16.67% of children
with HBV DNA positive sera. Full-length genome analysis revealed the homology of 99.62–100% in each motherchild pair. Genotype B was clarified to recombinant genotype B4/C2 and B2/C2. Double (48.39%) and combination
mutation (32.26%) were observed in core promoter region of HBV C1 strains.
Conclusions: This study showed the capable of DBS for large-scale molecular epidemiological study of HBV in
resource limited countries. Full-genome sequences yield the better understanding of sub-genotypes, their variants
and the degree of homology between strains isolated from mother-child pairs calls for effective strategies on
prevention, control and surveillance of mother-to-child HBV transmission in Cambodia. ...

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