Molecular Phylogenetic Analyses of Geographical and Ecological Speciation of Anaphalis margaritacea

概要

Poster
Poster 12

Molecular Phylogenetic Analyses of
Geographical and Ecological Speciation of Anaphalis margaritacea
Taishi HOSON1, Takuro ITO2, Shinji FUJII3, Daiki TAKAHASHI4, Ayumi MATSUO4,
Yoshihisa SUYAMA4 and Masayuki MAKI1,2
1

Graduate School of Life Sciences, Tohoku University
2
Botanical Gardens, Tohoku University
3
Department of Environmental Science, University of Human Environments
4
Graduate School of Agricultural Science, Tohoku University

Anaphalis margaritacea (Asteraceae) is a perennial species distributed widely from Asia to North America. In Japan, A.
margaritacea consists of three varieties; var. margaritacea distributed mainly in the mountain areas in eastern Japan, var. angustifolia
distributed in mountain areas in western Japan, var. yedoensis distributed in riverbanks covered with pebbles throughout Japan.
These varieties are considered to have been derived via different speciation patterns such as geographical and ecological speciations.
They seem to need taxonomic reexamination based on the molecular analysis because morphological intermediates between these
varieties have been sometimes reported. In this study, we collected these three varieties from all over Japan and examined the genetic
differentiation among the three varieties using the genome-wide SNPs by MIG-seq.
Analyses based on the SNPs showed that the populations of A. margaritacea var. angustifolia and var. yedoensis were
monophyletic, respectively and these lineages were contained with A. margaritacea var. margaritacea, suggesting that they are of
a single origin from A. margaritacea var. margaritacea. Population genetics analyses showed that the A. margaritacea complex
comprised three clades as a whole: “A. margaritacea var. margaritacea in Hokkaido” clade, “A. margaritacea var. margaritacea in
Honshu” clade, and “A. margaritacea var. yedoensis in Hokkaido” clade. Anaphalis margaritacea var. angustifolia was included
in “var. margaritacea in Honshu” clade. On the other hand, it is revealed that A. margaritacea var. yedoensis in Honshu have a
admixture of the two clades of A. margaritacea var. margaritacea in Honshu and A. margaritacea var. yedoensis in Hokkaido. Based
on these results, we will discuss differentiation processes among the three varieties of A. margaritacea. ...