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Notes

The authors declare no competing financial interest.

ACKNOWLEDGMENTS

This work was supported by a Grant-in-Aid for Scientific

Research on Innovative Areas “Chemistry for Multimolecular

Crowding Biosystems” (JSPS KAKENHI Grant no. 17H06356

to A.N.), a PRESTO (JST Grant no. JPMJPR19H9 to T.Y.), a

Medical Research and Development Program (AMED Grand

no. JP21he2302007 to T.Y. and A.N.), a SICORP (JST Grant

no. JPMJSC19E3 to T.Y.), a Grant-in-Aid for Young Scientists

(A) (JSPS KAKENHI Grant no. 17H04803 to T.Y.), a Grantin-Aid for Scientific Research (S) (JSPS KAKENHI Grant no.

18H05243 to T.Y.), a Grant-in-Aid for Exploratory Research

(JSPS KAKENHI Grant no. 20K21124 to T.Y.), and a Grantin-Aid for Young Scientists (B) (JSPS KAKENHI Grant no.

17K16643 to K.A.).

ABBREVIATIONS

AUC, area under the curve

BBB, blood−brain barrier

CI, confidence interval

CNS, central nervous system

COP, cyclo-olefin polymer

CT, computed tomography

DEM, differentially expressed microRNA

DNT, dysembryoplastic neuroepithelial tumor

EV, extracellular vesicle

FBS, fetal bovine serum

GBM, glioblastoma

GBO, glioblastoma organoid

GEO, Gene Expression Omnibus

GO, Gene Ontology

IDH, isocitrate dehydrogenase

IHC, immunohistochemistry

LASSO, least absolute shrinkage and selection operator

LGG, lower grade glioma

mRNA, messenger RNAs

miRNA, microRNA

MRI, magnetic resonance imaging

MVNT, multinodular and vacuolating neuronal tumor of

the cerebrum

N/A, not applicable

NCBI, National Center for Biotechnology Information

NHA, normal human astrocyte

NOS, not otherwise specified

nt, nucleotide

PBS, phosphate-buffered saline

PCR, polymerase chain reaction

PDMS, poly(dimethylsiloxane)

PEEK, polyether ether ketone

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