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大学・研究所にある論文を検索できる 「Protocol for the isolation of mouse senescence-associated CD4⁺ T cells using flow cytometry and functional assays」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

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Protocol for the isolation of mouse senescence-associated CD4⁺ T cells using flow cytometry and functional assays

Fukushima, Yuji Minato, Nagahiro Hattori, Masakazu 京都大学 DOI:10.1016/j.xpro.2023.102472

2023.09.15

概要

Senescence-associated (SA) CD4⁺ T cells, which increase with age, may underlie the development of autoimmunity and chronic inflammation, but their pathological function remains understudied. Here, we present a protocol to isolate CD153⁺ SA-T cells and evaluate their characteristic responses upon T cell receptor stimulation. We describe steps for the isolation of CD153⁺ SA-T cells using flow cytometry and in vitro culture with stimulatory antibodies against CD3, CD28, and CD153. We then detail the assessment of the proliferation capacity and cytokine production.

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参考文献

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STAR Protocols 4, 102472, September 15, 2023

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