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Figure legends
Figure 1. Immunolocalization of HURP protein in the human endometrium
HURP protein was localized in the nucleus of the endometrial epithelial and stromal
cells in all stages. Original magnification ×100. (A, C, E, G, I) Immunostaining with the
primary antibody for HURP. (B, D, F, H, J) Addition of blocking peptides for HURP to
the primary antibody for HURP. (EP, early-proliferative phase; LP, late-proliferative
phase; ES, early-secretory phase; MS, mid-secretory phase; LS, late-secretory phase)
Figure 2. Expression of HURP mRNA and protein levels in human endometrium in
various stages of the menstrual cycle
Endometrial tissues were divided into five groups, early-proliferative (EP), lateproliferative (LP), early-secretory (ES), mid-secretory(MS), late-secretory (LS) phase,
based on the endometrial tissues classified according to the last menstrual period and
histology according to the criteria of Noyes et al. [16]. (A) Uterine HURP mRNA
expression during the menstrual cycle was examined by quantitative RT-PCR. A
significant increase in HURP expression level was observed during the menstrual cycle
(＊P < 0.05, ＊＊P < 0.05 vs. EP). (B) Twenty micrograms of proteins were
immunoblotted with a HURP polyclonal antibody (upper panel). This membrane was
also reprobed with an anti-actin antibody, which was used as an internal control to
indicate relative loading of the samples (lower panel). (C) Western blot was used to
examine uterine HURP protein expression during the menstrual cycle. A significant
increase in HURP expression level was observed during the menstrual cycle (＊P < 0.05
vs. EP, LP, ES, and MS.).
Figure 3. Effect of sex steroid hormones E2 and P4 on regulation of HURP protein
expression
A), B) The cells were treated with DMSO (C, negative control), E2 (10-8 M), P4 (10-6M)
and combination of E2 (10-8M) and P4 (10-6M) for 2 days and harvested 24 h following
the final injection. C), D) The cells were treated with DMSO (C, negative control), E2
(10-9 to -7M), P4 (10-7 to -5M) for 2 days and harvested 24 h following the final injection.
Graphs represent the analysis of immunoblotting data. (*P < 0.05 vs. control)
Figure 1 Immunolocalization of HURP protein in the human endometrium
A (EP)
B (EP)
C (LP)
D (LP)
E (ES)
F (ES)
G (MS)
H (MS)
I (LP)
J (LP)
fi
Figure 2 Expression of HURP mRNA and protein levels in various stages of human
endometrium.
Figure 3 Effect of sex-steroid hormones E2 and P4 on regulation of HURP
protein expression
Figure 4 Effect of sex-steroid hormones E2 and P4 on regulation of HURP
protein expression
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