ウイルスファージ感染トランスクリプトームの分析
概要
令和4年度
京都大学化学研究所 スーパーコンピュータシステム 利用報告書
ウイルスファージ感染トランスクリプトームの分析
Analysis of the virophage infection transcriptome
京都大学 化学研究所 バイオインフォマティクスセンター 化学生命科学 CHEN JINGJIE
研究成果概要
We performed a time-course transcriptome analysis in a coinfection system of Acanthamoeba
polyphaga mimivirus (APMV) and Sputnik 3 virophage with Acanthamoeba castellanii as their
host and compared coinfection treatment to single infection of APMV. The overall sample
distances calculated by Eluclidean method indicates that sputnik mainly affected gene expression
of APMV from the mid-phase (about 6 hours after infection). Meanwhile, according to the
transcriptome analysis results, combining with the limited gene annotation of virophage, we
found that the replication of sputnik seems starts from 3 hpi and continue to 6hpi which is
coordinate with the activate DNA replication time of APMV. For genes about DNA packaging
and capsid formation, it shows activity increase in the late part of infection (6hpi and 12hpi),
which indicates the quite close dependency of sputnik production with APMV and possibly more
rely on the mid-late phase genes of APMV replication. However, due to the lack of biological
replicates, we cannot make definite conclusion about the results and also cannot do any further
statistical analysis. We have sent new samples that contain three replicates for sequencing to
confirm the observation and may be able to discovering something new from them. ...