Functional analysis of pollutant nicotine-CHRNA1 and identification of prognosis-related genes in melanoma using zebrafish and in silico approaches

概要

Thesis Abstract
Functional analysis of pollutant nicotine-CHRNA1 and identification of prognosis-related
genes in melanoma using zebrafish and in silico approaches
(ゼブラフィッシュとインシリコ系を用いた、メラノーマにおけるニコチン‐ニコチン性ア
セチルコリン受容体 A1 の機能解析と予後関連遺伝子の同定)
MOHAMED NABIL BAKR ABDELRAHMAN
Department of Biological Science,
Graduate School of Science,
Hiroshima University

Content of the thesis
Chapter 1. General Introduction ...……………………………….....................
5
References …………...………………………………………......... 8
Chapter 2. CHRNA1 and its correlated-myogenesis/muscle contraction genes are
prognosis-related markers of metastatic melanoma ………………. 12
Introduction …………………………………………………..........
Material and Methods ……………………………...………...........
Results ………………………………………………………..........
Discussion …………………………………………………………

13
15
22
28

References ………………………………………………………… 31
Figures …………………………………………………………….. 37
Supplementary Figures …………………………………...………. 44
Tables ……………………………………………………………... 49
Chapter 3. Analysis of Melanoma Gene Expression Signatures at the Single-Cell
Level Uncovers 45-Gene Signature Related to Prognosis …...……

50

Introduction ………………………………………………………..
Material and Methods ……………………………………..............
Results ………………………………………………………..........
Discussion …………………………………………………………

51
54
59
66

References ………………………………………………………… 71
Figures …………………………………………………………….. 82
Supplementary Figures …………………………………...………. 94
Supplementary Tables …………………………...………………... 99
Chapter 4. General Discussion ...……………...…...…………………............. 101
References …………...………………………………………......... 107
Acknowledgement …………...………………………………………............................ 114