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大学・研究所にある論文を検索できる 「Species identification of β-hemolytic streptococci from diseased companion animals and their antimicrobial resistance patterns in Japan (2021)」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

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Species identification of β-hemolytic streptococci from diseased companion animals and their antimicrobial resistance patterns in Japan (2021)

Kurita, Goro Tsuyuki, Yuzo Shibata, Sachiko Itoh, Megumi Goto, Mieko Yoshida, Haruno Takahashi, Takashi 北海道大学

2022.02

概要

We aimed to identify species and evaluate antimicrobial resistance (AMR) of ß-hemolytic streptococci recovered from Japanese companion animals in 2021. Strains were recovered from clinical specimens of 109 companion animals that exhibited symptoms/signs between April and May, 2021. We identified strains by 16S rRNA sequencing and evaluated their AMR phenotypes using the broth microdilution method. Macrolide/lincosamide/tetracycline resistance genes, including erm(A), erm(B), and mef(A), in addition to tet(M), tet(O), tet(K), tet(L), and tet(S), were amplified by polymerase chain reaction. The 16S rRNA sequencing identified ß-hemolytic streptococcal strains as Streptococcus canis (n = 102, 93.6%), S. dysgalactiae subsp. equisimilis (n = 4), S. agalactiae (n = 2), and S. dysgalactiae subsp. dysgalactiae (n = 1). Overall AMR rates were 34.9% for minocycline, 22.0% for erythromycin, 22.9% for azithromycin, 21.1% for clindamycin, and 10.1% for levofloxacin. We found macrolide/lincosamide/tetracycline resistance gene detection of tet(M) (9.2%), tet(O) (26.6 %), tet(L) (1.8%), erm(B) (22.0%), and mef(A) (1.8%). Our findings support the unique features of β-hemolytic streptococci from companion animals (dominant S. canis isolation and dominant resistance to tetracycline), along with dominant tetracycline resistance genes, in agreement with those of ß-hemolytic streptococci in 2017.

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参考文献

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