1. Tao Q, Chan AT. Nasopharyngeal carcinoma: molecular pathogenesis and therapeutic developments. Expert Rev Mol Med. 2007;9:1-24.
2. Klutstein M, Nejman D, Greenfield R, Cedar H. DNA methylation in
cancer and aging. Cancer Res. 2016;76:3446-3450.
3. Mo Y, Midorikawa K, Zhang Z, et al. Promoter hypermethylation of
Ras-related GTPase gene RRAD inactivates a tumor suppressor function in nasopharyngeal carcinoma. Cancer Lett. 2012;323:147-154.
4. Zhao W, Mo Y, Wang S, et al. Quantitation of DNA methylation in
Epstein-Barr virus–associated nasopharyngeal carcinoma by bisulfite amplicon sequencing. BMC Cancer. 2017;17:489.
5. Wang S, Xiao X, Zhou X, et al. TFPI-2 is a putative tumor suppressor
gene frequently inactivated by promoter hypermethylation in nasopharyngeal carcinoma. BMC Cancer. 2010;10:617.
6. Zhou X, Xiao X, Huang T, et al. Epigenetic inactivation of follistatin-like 1 mediates tumor immune evasion in nasopharyngeal carcinoma. Oncotarget. 2016;7:16433.
7. Schübeler D. Function and information content of DNA methylation. Nature. 2015;517:321-326.
8. Michalak EM, Burr ML, Bannister AJ, Dawson MA. The roles of
DNA, RNA and histone methylation in ageing and cancer. Nat Rev
Mol Cell Biol. 2019;20:573-589.
9. Stroun M, Anker P, Maurice P, Lyautey J, Lederrey C, Beljanski M.
Neoplastic characteristics of the DNA found in the plasma of cancer patients. Oncology. 1989;46:318-322.
10. Ordway JM, Bedell JA, Citek RW, et al. Comprehensive DNA methylation profiling in a human cancer genome identifies novel epigenetic targets. Carcinogenesis. 2006;27:2409-2423.
11. Khachatoorian R, Dawson D, Maloney EM, et al. SAMe treatment
prevents the ethanol-induced epigenetic alterations of genes in the
Toll-like receptor pathway. Exp Mol Pathol. 2013;94:243-246.
12. Sundrani D, Narang A, Mehendale S, Joshi S, Chavan-Gautam P.
Investigating the expression of MMPs and TIMPs in preterm placenta and role of CpG methylation in regulating MMP-9 expression.
IUBMB Life. 2017;69:985-993.
13. Xavier FC, Destro MF, Duarte CM, Nunes FD. Epigenetic repression of
HOXB cluster in oral cancer cell lines. Arch Oral Biol. 2014;59:783-789.
14. Alghamian Y, Abou Alchamat G, Murad H, Madania A. Effects of
γ-radiation on cell growth, cell cycle and promoter methylation of
22 cell cycle genes in the 1321NI astrocytoma cell line. Adv Med Sci.
2017;62:330-337.
15. Oakes CC, La Salle S, Trasler JM, Robaire B. Restriction digestion
and real-time PCR (qAMP). In: Tost J, ed. DNA Methylation. New
York: Humana Press, c/o Springer Science+Business Media, LLC;
2009:271-280.
16. Zhao W, Ma N, Wang S, et al. RERG suppresses cell proliferation,
migration and angiogenesis through ERK/NF-κB signaling pathway
in nasopharyngeal carcinoma. J Exp Clin Cancer Res. 2017;36:88.
17. Pizzamiglio S, Zanutto S, Ciniselli CM, et al. A methodological procedure for evaluating the impact of hemolysis on circulating microRNAs. Oncol Lett. 2017;13:315-320.
18. Šimundić A-M. Measures of diagnostic accuracy: basic definitions.
EJIFCC. 2009;19:203-211.
19. Wang H-Y, Hung C-C, Chen C-H, et al. Increase trichomonas vaginalis detection based on urine routine analysis through a machine
learning approach. Sci Rep. 2019;9:11074.
20. Øster B, Thorsen K, Lamy P, et al. Identification and validation of
highly frequent CpG island hypermethylation in colorectal adenomas and carcinomas. Int J Cancer. 2011;129:2855-2866.
21. Arai E, Chiku S, Mori T, et al. Single-CpG-resolution methylome
analysis identifies clinicopathologically aggressive CpG island methylator phenotype clear cell renal cell carcinomas. Carcinogenesis.
2012;33:1487-1493.
2 2. Tian Y, Arai E, Gotoh M, Komiyama M, Fujimoto H, Kanai Y.
Prognostication of patients with clear cell renal cell carcinomas
based on quantification of DNA methylation levels of CpG island
methylator phenotype marker genes. BMC Cancer. 2014;14:772.
23. Yeh C-M, Chen P-C, Hsieh H-Y, et al. Methylomics analysis identifies ZNF671 as an epigenetically repressed novel tumor suppressor
and a potential non-invasive biomarker for the detection of urothelial carcinoma. Oncotarget. 2015;6:29555-29572.
24. Hansel A, Steinbach D, Greinke C, et al. A promising DNA methylation signature for the triage of high-risk human papillomavirus
DNA-positive women. PLoS ONE. 2014;9:e91905.
25. Mase S, Shinjo K, Totani H, et al. ZNF671 DNA methylation as a molecular predictor for the early recurrence of serous ovarian cancer.
Cancer Sci. 2019;110:1105-1116.
26. Lleras RA, Adrien LR, Smith RV, et al. Hypermethylation of a cluster of Krüppel-type zinc finger protein genes on chromosome
19q13 in oropharyngeal squamous cell carcinoma. Am J Pathol.
2011;178:1965-1974.
27. Zhang J, Wen X, Liu NA, et al. Epigenetic mediated zinc finger protein 671 downregulation promotes cell proliferation and tumorigenicity in nasopharyngeal carcinoma by inhibiting cell cycle arrest. J
Exp Clin Cancer Res. 2017;36:147.
28. Morandi L, Gissi D, Tarsitano A, et al. CpG location and methylation
level are crucial factors for the early detection of oral squamous
cell carcinoma in brushing samples using bisulfite sequencing of a
13-gene panel. Clin Epigenetics. 2017;9:85.
29. Nakagawa T, Matsusaka K, Misawa K, et al. Frequent promoter hypermethylation associated with human papillomavirus infection in
pharyngeal cancer. Cancer Lett. 2017;407:21-31.
3 0. Ahmed D, Danielsen SA, Aagesen TH, et al. A tissue-based comparative effectiveness analysis of biomarkers for early detection of
colorectal tumors. Clin Transl Gastroenterol. 2012;3:e27.
31. Tsai M-H, Chen W-C, Yu S-L, et al. DNA hypermethylation of
SHISA3 in colorectal cancer: an independent predictor of poor
prognosis. Ann Surg Oncol. 2015;22:1481-1489.
32. Shen Z, Zhou C, Li J, et al. SHISA3 promoter methylation is a potential diagnostic and prognostic biomarker for laryngeal squamous
cell carcinoma. Biomed Res Int. 2017;2017:9058749.
33. Zhang J, Li YQ, Guo R, et al. Hypermethylation of SHISA3 promotes
nasopharyngeal carcinoma metastasis by reducing sgsm1 stability.
Cancer Res. 2019;79:747-759.
3 4. Barault L, Amatu A, Siravegna G, et al. Discovery of methylated
circulating DNA biomarkers for comprehensive non-invasive monitoring of treatment response in metastatic colorectal cancer. Gut.
2018;67:1995-2005.
35. Usadel H, Brabender J, Danenberg KD, et al. Quantitative adenomatous polyposis coli promoter methylation analysis in tumor tissue, serum, and plasma DNA of patients with lung cancer. Cancer
Res. 2002;62:371-375.
36. Mitchell S, Ho T, Brown G, et al. Evaluation of methylation biomarkers for detection of circulating tumor DNA and application to colorectal cancer. Genes. 2016;7:125.
2545
XU et al.
37. Snyder MW, Kircher M, Hill AJ, Daza RM, Shendure J. Cell-free
DNA comprises an in vivo nucleosome footprint that informs its
tissues-of-origin. Cell. 2016;164:57-68.
How to cite this article: Xu Y, Zhao W, Mo Y, et al. Combination
of RERG and ZNF671 methylation rates in circulating cell-free
DNA: A novel biomarker for screening of nasopharyngeal
S U P P O R T I N G I N FO R M AT I O N
Additional supporting information may be found online in the
Supporting Information section.
carcinoma. Cancer Sci. 2020;111:2536–2545. https://doi.
org/10.1111/cas.14431
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