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大学・研究所にある論文を検索できる 「Construction of Henosepilachna Vigintioctomaculata cDNA Library and Identification of dsRNA Injection–Induced RNAi Effect」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。
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Construction of Henosepilachna Vigintioctomaculata cDNA Library and Identification of dsRNA Injection–Induced RNAi Effect

KIM, Jeong Hee KWON, Hye Ri YU, Yong Man YASUNAGA–AOKI, Chisa 青木, 智佐 アオキ, チサ YOUN, Young Nam 九州大学

2020.02

概要

Henosepilachna vigintioctomaculata is a pest that damages plants of the Solanaceae family. To con- trol of H. vigintioctomaculata through RNAi, which has received much attention in recent years, we inves- tigated the effect of RNAi by injecting dsRNAs and constructing a cDNA library for selecting the genes to be targeted by RNAi. The N20 primer was used to synthesize cDNA of different sizes by removing the poly A. After amplification, PCR products of sizes varying between 200 and 600 bp were obtained. For the efficient cloning of the different genes, the products were cloned into a pDONR 207 vector using the Gateway system for constructing the cDNA library. The cDNA library thus constructed had a titer of 3.15 × 105 cfu/ml. The genes from the cDNA library were cloned into a LITMUS 28i vector containing the T7 promoter for synthe- sizing dsRNA. Electro–transformation was performed in the E. coli cells, and 48 colonies were randomly selected to confirm the size and duplication of the insert. The insert sizes varied between 100 and 500 bp and the different genes were cloned without duplicating the sequences. The results showed that 23 genes were those of insects, including those of the order Coleoptera, while the rest were genes from non–insect species. Using the T7 promoter of the LITMUS 28i vector, the genes with identified sequence and gene information were used for synthesizing 200–600 bp dsRNAs suitable for RNAi. Most of the larvae injected with Hv1, Hv4, and Hv7 genes turned black and died within 7–10 days after injection.

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参考文献

Al–Ayedh, H., M. Rizwan–ul–Haq, A. Hussain and A. M Aljabr 2016. Insecticidal potency of RNAi–based catalase knockdown in Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae). Pest Man. Sci., 72(11): 2118–2127

Bautista, M. A. M., T. Miyata, K. Miura and T. Tanaka 2009. RNA interference–mediated knockdown of a cytochrome P450, CYP6BG1, from the diamondback moth, Plutella xylostella, reduces larval resistance to permethrin. Insect Biochem. Mol. Biol., 39(1): 38–46

Bell’es, X 2010 Beyond Drosophila: RNAi in vivo and functional genomics in insects. Ann. Rev. Entomol., 55: 111–128

Birnboim, H. C. and J. Doly 1979. A rapid alkaline extraction pro- cedure for screening recombinant plasmid DNA. Nuc. Acid. Res., 7(6): 1513–1523

Bolognesi, R., P. Ramaseshadri, J. Anderson, P. Bachman, W. Clinton, R. Flannagan, O. Ilagan, C. Lawrence, S. Levine, W. Moar, G. Mueller, J. Tan, J. Uffman, E. Wiggins, G. Heck and G. Segers 2012. Characterizing the mechanism of action of dou- ble-stranded RNA activity against western corn rootworm (Diabrotica virgifera virgifera LeConte). PLoS One., 7(10): e47534

Burand, J. P. and W. B. Hunter 2013. RNAi: Future in insect management. J. Invert. Pathol., 112(1): S68–S74

Das, S., N. Debnath, Y. Cui, J. Unrine and S. R. Palli 2015. Chitosan, carbon quantum dot, and silica nanoparticle mediated dsRNA delivery for gene silencing in Aedes aegypti: A compara- tive analysis. ACS Appl. Mater. Interfaces., 7(35): 19530– 19535

Ekert, E. V., C. A. Powell, R. G. Shatters Jr. and D. Borovsky 2014. Control of larval and egg development in Aedes aegypti with RNA interference against juvenile hormone acid methyl trans- ferase. J. Insect Physiol., 70: 143–150

Hartley, J. L., G. F. Temple and M. A. Brasch 2000. DNA cloning using in vitro site–specific recombination. Genome Res., 10: 1788–1795

Hori, M., Y. Nakamura, Y. Fujii, Y. Suzuki and K. Matsuda 2011. Chemicals affecting the feeding preference of the Solanaceae– feeding lady beetle Henosepilachna vigintioctomaculata (Coleoptera: Coccinellidae). J. Appl. Entomol., 135: 121–131

Hoshikawa, K 1983. Host–race formation and speciation in the Henosepilachna vigintioctomaculata complex (Coleoptera, Coccinellidae): I. Host–plant ranges and food–preference types. Jap. J. Entomol., 51(2): 254–264

Hu, X., N. M. Richtman, J. Z. Zhao, K. E. Duncan, X. Niu, L. A. Procyk, M. A. Oneal, B. M. Kernodle, J. P. Steimel, V. C. Crane, G. Sandahl, J. L. Ritland, R. J. Howard, J. K. Presnail, A. L. Lu and G. Wu 2016. Discovery of midgut genes for the RNA interference control of corn rootworm. Sci. Rep., 6: 30542. Doi: 10.1038/srep30542

Joazeiro, C. A. P. and A. M. Weissman 2000. RING finger pro- teins: Mediators of ubiquitin ligase activity. Cell, 102(5): 549– 552

Jung, Y. B. 2016. cDNA library construction and application of RNA interference for gene functional analysis in Harmonia axyridis. Master Thesis, Chungnam National University, Korea. pp 91

Kang, C. Y., T. H. Ryu, Y. B. Jung, N. Y. Ko, H. R. Kwon, M. J. Seo, Y. M. Yu, Y. N. Youn and Y. G. Kim 2014. Developmental char- acteristics of Henosepilachna vigintioctomaculata under dif- ferent temperatures and control effect of eco–friendly agricul- tural materials on Lycium chinense. CNU J. Agricul. Sci., 41(4): 361–367

Karunker, I., J. Benting, B. Lueke, T. Ponge, R. Nauen, E. Roditakis, J. Vontas, K. Gorman, I. Denholm and S. Morin 2008. Over– expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae). Insect Biochem. Mol. Biol., 38(6): 634–644

Ko, N. Y. and Y. N. Youn 2015. Change of population density of tobacco whitefly (Bemisia tabaci, Aleyrodidae, Hemiptera) by RNAi. CNU J. Agricul. Sci., 42(1): 7–13

Kobayashi, N., K. Tamura, T. Aotsuka and H. Katakura 1998. Molecular phylogeny of twelve Asian species of epilachnine ladybird beetles (Coleoptera, Coccinellidae) with notes on the direction of host shifts. Zool. Sci., 15: 147–151

Kwon, M., J. I. Kim and J. S. Kim 2010. Ecological characteristics of 28–spotted larger lady beetle, Henosepilachna vigintiocto-maculata (Motschulsky) (Coleoptera: Coccinellidae) and its seasonal fluctuation in Gangneung, Korea. Korean J. Appl. Entomol., 49(3): 199–104

Lee, J. H., D. K. Reed, H. P. Lee and R. W. Carlson 1988. Parasitoids of Henosepilachna vigintioctomaculata (Moschulsky) (Coleoptera: Coccinellidae) in Kyonggido area, Korea. Korean J. Appl. Entomol., 27(1): 28–34

Li, J., X. Li, R. Bai, Y. Shi, Q. Tang, S. An, Q. Song and F. Yan 2014. RNA interference of the P450 CYP6CM1 gene has different effi- cacy in B and Q biotypes of Bemisia tabaci. Pest Man. Sci., 71: 1175–1181

Liu, X. M., B. X. Zhang, S. G. Li, X. J. Rao, D. M. Wang, X. X. Hu and S. Liu 2016. Knockdown of the olfactory co–receptor Orco impairs mate recognition in Tenebrio molitor (Coleoptera: Tenebrionidae). J. Asia–Pacific Entomol., 19(2): 503–508

Lycett, G. J., L. A. McLaughlin, H. Ranson, J. Hemingway, F. C. Kafatos, T. G. Loukeris and M. J. I. Paine 2006. Anopheles gambiae P450 reductase is highly expressed in oenocytes and in vivo knockdown increases permethrin susceptibility., Insect Mol. Biol., 15(3): 321–327

Mao, S., W. Sun and T. Kissel 2010. Chitosan–based formulations for delivery of DNA and siRNA. Adv. Drug Del. Rev., 62(1): 12–27

Mao, Y. B., X. Y. Tao, X. Y. Xue, L. J. Wang and X. Y. Chen 2011. Cotton plants expressing CYP6AE14 double–stranded RNA show enhanced resistance to bollworms. Trans. Res., 20(3): 665–673

Niimi, T., H. Kuwayama and T. Yaginuma 2005. Larval RNAi applied to the analysis of postembryonic development in the ladybird beetle, Harmonia axyridis. J. Insect Biotech. Sericol., 74(3): 95–102

Pitino, M., A. D. Coleman, M. E. Maffei, C. J. Ridout and S. A. Hogenhout 2011. Silencing of aphid genes by dsRNA feeding from plants. PLoS One., 6(10): e25709

Prentice, K., O. Christiaens, I. Pertry, A. Bailey, C. Niblett, M. Ghislain, G. Gheysen and G. Smagghe 2017. RNAi–based gene silencing through dsRNA injection or ingestion against the African sweet potato weevil Cylas puncticollis (Coleoptera: Brentidae). Pest Man. Sci., 73(1): 44–52

Raza, A., H. J. Malik, M. Shafiq, I. Amin, J. A. Scheffler, B. E. Scheffler and S. Mansoor 2016. RNA interference based approach to down regulate osmoregulators of whitefly (Bemisia tabaci): Potential technology for the control of whitefly. PLoS One., 11(4): e0153883

Saleh, M. C., R. P. van Rij, A. Hekele, A. Gillis, E. Foley, P. H. O’Farrell and R. Andino 2006. The endocytic pathway medi- ates cell entry of dsRNA to induce RNAi silencing. Nat. Cell Biol., 8: 793–802

Song, P., Q. Y. Wang, H. X. Wu, X. J. Lu and Y. Wang 2008. Identification of the cry gene in Bacillus thuringiensis strain WZ–9 and its toxicity against Henosepilachna vigintioctomac- ulata. Chin. J. Agricul. Biotech., 5(3): 245–250

Yu, N., O. Christiaens, J. Liu, J. Niu, K. Cappelle, S. Caccia, H. Huvenne and G. Smagghe 2013. Delivery of dsRNA for RNAi in insects: an overview and future directions. Insect Sci., 20: 4–14

Zhao, Y., G. Yang, G. Wang–Pruski and M. You 2008. Phyllotreta striolata (Coleoptera: Chrysomelidae): Arginine kinase cloning and RNAi–based pest control. Euro. J. Entomol., 105(5): 815– 822

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