Spiranthes hachijoensis (Orchidaceae), a new species within the S. sinensis species complex in Japan, based on morphological, phylogenetic, and ecological evidence

Barrett CF, Freudenstein JV (2011) An integrative approach to delimiting species in a rare but widespread mycoheterotrophic orchid.

Mol Ecol 20:2771–2786

Barrett CF, Santee MV, Fama NM et al (2022) Lineage and role in

integrative taxonomy of a heterotrophic orchid complex. Mol Ecol

31:4762–4781. https://​doi.​org/​10.​1111/​mec.​16617

Botes C, van der Niet T, Cowling RM, Johnson SD (2020) Is biodiversity underestimated by classical herbarium-based taxonomy?

A multi-disciplinary case study in Satyrium (Orchidaceae). Bot J

Linn Soc 194:342–357

Catling PM (1982) Breeding systems of northeastern North American

Spiranthes (Orchidaceae). Can J Bot 60:3017–3039. https://​doi.​

org/​10.​1139/​b82-​358

Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue.

Focus 12:13–15

Dueck LA, Aygoren D, Cameron KM (2014) A molecular framework

for understanding the phylogeny of Spiranthes (Orchidaceae), a

347

cosmopolitan genus with a North American center of diversity.

Am J Bot 101:1551–1571

Frericks J, Munkacsi A, Ritchie P et al (2018) Phylogenetic affinities and in vitro seed germination of the threatened New Zealand

orchid Spiranthes novae-zelandiae. N Z J Bot 56:91–108. https://​

doi.​org/​10.​1080/​00288​25X.​2017.​14183​98

Hayakawa H, Ohga K, Miyata H et al (2013) Phylogenetic background

of a glabrous individual of Spiranthes sinensis var. amoena

(Orchidaceae) collected in Kochi prefecture, Japan. J Phytogeogr

Taxon 61:45–50

Hsu TC, Chung SW (2014) Spiranthes hongkongensis (Orchidaceae):

Newly recorded from Taiwan. Taiwan J Biodivers 16:405–408

Hsu T-C, Chung S-W (2016) The illustrated flora of Taiwan. Owl Publishing House, Taipei

Hu SY, Barretto G (1976) New species and varieties of Orchidaceae

from Hong Kong. Chung Chi J 13:1–34

Huson DH, Bryant D (2006) Application of phylogenetic networks in

evolutionary studies. Mol Biol Evol 23:254–267

IUCN (2019) Guidelines for using the IUCN red list categories and

criteria. Version 14. http://​www.​iucnr​edlist.​org/​docum​ents/​RedLi​

stGui​delin​es.​pdf. Accessed 6 Oct 2022

Iwata T, Nagasaki O, Ishii HS, Ushimaru A (2012) Inflorescence architecture affects pollinator behaviour and mating success in Spiranthes sinensis (Orchidaceae). New Phytol 193:196–203. https://d​ oi.​

org/​10.​1111/j.​1469-​8137.​2011.​03892.x

Lin TP, Lin WM (2011) Newly discovered native orchids of Taiwan

(IV). Taiwania 56:315–322

Lowry DB, Modliszewski JL, Wright KM et al (2008) The strength

and genetic basis of reproductive isolating barriers in flowering

plants. Philos Trans R Soc B Biol Sci 363:3009–3021. https://d​ oi.​

org/​10.​1098/​rstb.​2008.​0064

Maekawa F (1971) The Wild Orchids of Japan in Color. Seibundoshinkosha, Tokyo

Pace MC, Cameron KM (2017) The systematics of the Spiranthes

cernua species complex (Orchidaceae): Untangling the Gordian

Knot. Syst Bot 42:1–30. https://​doi.​org/​10.​1600/​03636​4417X​

696528

Pace MC, Cameron KM (2020) The enduring value of specimen-based

research: a response to Surveswaran. Bot J Linn Soc 194:382–384

Pace MC, Orzell SL, Bridges EL, Cameron KM (2017) Spiranthes

igniorchis (Orchidaceae), a new and rare cryptic species from the

south-central Florida subtropical grasslands. Brittonia 69:323–339

Pace MC, Giraldo G, Frericks J et al (2019) Illuminating the systematics of the Spiranthes sinensis species complex (Orchidaceae):

ecological speciation with little morphological differentiation. Bot

J Linn Soc 189:36–62

Rochette NC, Rivera-Colón AG, Catchen JM (2019) Stacks 2: analytical methods for paired-end sequencing improve RADseq-based

population genomics. Mol Ecol 28:4737–4754

Satomi N (1982) Orchidaceae. In: Satake Y, Ohwi J, Kitamura S,

Watami S and Tominari T (eds) Wild flowers of Japan: herbaceous plants (including dwarf subshrubs). Heibonsha, Tokyo, pp

187–235

Schlechter A (1906) Spiranthes neocaledonica. Bot Jahrb Syst 39:51

Stamatakis A (2014) RAxML version 8: a tool for phylogenetic

analysis and post-analysis of large phylogenies. Bioinformatics

30:1312–1313

Suetsugu K (2022) Living in the shadows: Gastrodia orchids lack functional leaves and open flowers. Plants People Planet 4:418–422.

https://​doi.​org/​10.​1002/​ppp3.​10281

Suetsugu K, Abe Y (2021) Unexpected contribution of the introduced

honeybee Apis mellifera to high fruit set in Spiranthes australis.

Entomol News 129:559–563. https://​doi.​org/​10.​3157/​021.​129.​

0511

Suetsugu K, Hayakawa H (2016) Phylogenetic background of the

glabrous and early blooming Spiranthes sinensis (Orchidaceae)

13

348

collected in Kumamoto Prefecture, Japanese mainland. J Jpn Bot

91:331–336

Suetsugu K, Hayakawa H (2019) Spiranthes hongkongensis SY Hu

& Barretto (Orchidaceae): Newly discovered orchid on Ishigaki Island, Ryukyu Islands, Japan. Acta Phytotaxon Geobot

70:205–208

Suetsugu K, Hirota SK, Suyama Y (2021) First record of Goodyera ×

tamnaensis (Orchidaceae) from Boso Peninsula, Chiba Prefecture, Japan, based on morphological and molecular data. Taiwania

66:113–120

Suetsugu K, Hirota SK, Hsu T-C et al (2023) Monotropastrum kirishimense (Ericaceae), a new mycoheterotrophic plant from Japan

based on multifaceted evidence. J Plant Res 136:3–18. https://d​ oi.​

org/​10.​1007/​s10265-​022-​01422-8

Suetsugu K, Hsu TC (2023) Taxonomic revision of the genus Spiranthes (Orchidaceae) in Taiwan. Phytotaxa 578:1–10. https://​doi.​

org/​10.​11646/​phyto​taxa.​306.3.4

Suetsugu K, Lin J-Z, Hsu T-C, Hayakawa H (2020) Interspecific

hybridization between Spiranthes australis and S. sinensis (Orchidaceae) in southern Taiwan. Acta Phytotaxon Geobot 71:177–184.

https://​doi.​org/​10.​18942/​apg.​201922

Sun M (1996) The allopolyploid origin of Spiranthes hongkongensis

(Orchidaceae). Am J Bot 83:252–260. https://​doi.​org/​10.​1002/j.​

1537-​2197.​1996.​tb127​02.x

Surveswaran S, Kumar P, Sun M (2017) Spiranthes himalayensis

(Orchidaceae, Orchidoideae) a new species from Asia. PhytoKeys 89:115–128

Surveswaran S, Gowda V, Sun M (2018) Using an integrated approach

to identify cryptic species, divergence patterns and hybrid species

13

Journal of Plant Research (2023) 136:333–348

in Asian ladies’ tresses orchids (Spiranthes, Orchidaceae). Mol

Phylogenet Evol 124:106–121. https://​doi.​org/​10.​1016/j.​ympev.​

2018.​02.​025

Surveswaran S, Gowda V, Sun M (2020) Cryptic species and taxonomic troubles: a rebuttal of the systematic treatment of the Asian

ladies’ tresses orchids (Spiranthes species; Orchidaceae) by Pace

et al. (2019). Bot J Linn Soc 194:375–381

Suyama Y, Hirota SK, Matsuo A et al (2022) Complementary combination of multiplex high-throughput DNA sequencing for molecular

phylogeny. Ecol Res 37:171–181. https://​doi.​org/​10.​1111/​1440-​

1703.​12270

Tao Z-B, Ren Z-X, Bernhardt P et al (2018) Does reproductive isolation reflect the segregation of color forms in Spiranthes sinensis

(Pers.) Ames complex (Orchidaceae) in the Chinese Himalayas?

Ecol Evol 8:5455–5469. https://​doi.​org/​10.​1002/​ece3.​4067

Thiers B (2022) Index Herbariorum, New York Botanical Garden.

http://​sweet​gum.​nybg.​org/​scien​ce/​ih/. Accessed 6 Oct 2022

Tsukaya H (1994) Spiranthes sinensis var. amoena in Japan contains

two seasonally differentiated groups. J Plant Res 107:187–190

Tsukaya B (2005) Molecular variation of Spiranthes sinensis (Orchidaceae) in Japan, with special reference to systematic treatment

of seasonally differentiated groups and a dwarf form, f. gracilis,

from Yakushima Island. J Plant Res 118:13–18

Publisher's Note Springer Nature remains neutral with regard to

jurisdictional claims in published maps and institutional affiliations.

...