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Acknowledgements

I would like to express my deep gratitude to my supervisor Dr. Wataru Kitagawa,

without his support, my research would have not proceeded to this point. Whose expertise,

patience and generous support made everything possible during my study in the graduate

school. I learned a lot through these five years with him, not only the experimental

techniques, but also the techniques on logical thinking, critical thinking, and “scientific”

presentation. His taught will surely be my treasure for the rest of my life, no matter what

kind of career I would choose in the future. I can’t thank him enough for all his efforts

and hard works which made my academic career to be exiting and meaningful.

I appreciate Dr. Yoichi Kamagata for his supports. He was the motivation for me to

study in the graduate school of Agriculture in Hokkaido university and to work on the

cultivation of uncultured microbes. For these years, no matter how busy he was, he always

cared about me and my experiments, which was the strongest mental support. It was my

pleasure to be the student of the greatest microbiologist, and I would be pleased if my

works would be his aid in the future.

Dr. Yoshitomo Kikuchi and Dr. Souichiro Kato had helped me a lot on my studies.

Although our researching themes were slightly different, they always treated my

problems as theirs and gave me valuable suggestions. They also kindly welcomed me to

their fields, and they always gave me opportunities to talk with their co-workers. Which

had widened my perspectives and helped me to gain wide variety of knowledges.

The days I spent in the Hokkaido center of National Institute of Advanced Industrial

Science and Technology (AIST Hokkaido) were always exiting because of the people I

met there. I was always surrounded by the talented scientists with different expertise,

81

which was the biggest advantage that I got. They were always friendly and patient,

wishing my success on my study in the graduate school. I really appreciate every staff

who supported my career, especially, I really appreciate Dr. Kensuke Igarashi, Dr.

Ryosuke Nakai, and Dr. Shusei Kanie for their supports. I also feel many thanks to Ms.

Miyako Hata, who supported me a lot in the laboratory. She was the most patient teacher

on the operation of the laboratory equipment and without her, I wasn’t able to complete

the identification of more than 1,200 bacterial colonies, which was one of the biggest

experiments in my life.

I appreciate Prof. Teruo Sone for his warm supports. Although I had no opportunity to

work on his topics, he always kindly welcomed me to his field of study, letting me to

participate in his students’ seminars and journal clubs. With him and his students, my

days were always brilliant. Current and former students in the laboratory of Applied

Molecular Microbiology (also known as oukin laboratory), were always supportive and

the days I spent with them were the precious experiences.

My appreciation also extends to Dr. Seonghan Jang, who was the most respectful

“senpai” that I had met in my life. We discussed a lot on our experiments inside and

outside of the laboratory, and his attitudes toward his career had always motivated me to

do better. I will never forget the days we spent with our common friend Mr. Kota Ishigami.

Last but not the least, my deep appreciation goes to my family members. My life

wouldn’t have been this complete without their understanding and supports. My gratitude

extends to my deceased grandfather Akiyoshi Sano, who gave me opportunities for

education and to be like him is my lifelong goal.

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