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Figure Legends

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Fig. 1. Administration of I. multifiliis in the fin cavity. Images of calcein-labeled

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theronts (A) and a trophont (B). I. multifiliis antigens were injected into one dorsal fin

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compartment (white arrow) (C). Observation of live theronts administered in the fin

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cavity (D: bright field; E: dark field).

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Fig. 2. Percentages of CD8α+ T-cells (A), CD4+ T-cells (B), IgM+ B-cells (C),

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macrophages (D), and neutrophils (E) from the fin cavity. Open, shaded, dotted, closed,

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and hatched bars indicate the percentages from non-administered, PBS-, live theront-,

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killed-theront-, and trophont-administered fish, respectively. Results are presented as the

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means of three individual fish, and error bars indicate the standard deviation (SD). The

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asterisks indicate significant differences between the control (PBS-administered fish) at

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each time point (*P < 0.05).

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Fig. 3. Quantitative expression profiles of T-bet, GATA-3, IgM, and iNOS in cells from

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the fin cavity. Open, shaded, dotted, closed, and hatched bars indicate the percentages

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from non-administered, PBS-, live theront-, killed-theront-, and trophont-administered

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fish, respectively. Results are presented as the means of three individual fishes, and

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error bars indicate the SD. The asterisks indicate significant differences between the

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control (PBS-administered fish) at each time point (*P < 0.05).

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