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Anatomical and Electrophysiological Analysis of Cholinergic Parabigemino-Collicular Projection

Tokuoka, Kota 京都大学 DOI:10.14989/doctor.k23340

2021.03.23

概要

Superior colliculus (SC) is a midbrain structure that integrates sensory inputs and generates motor commands to initiate innate motor behaviors. Its retinorecipient superficial layers (sSC) receive dense cholinergic projections from the parabigeminal nucleus (PBN). It is known that acetylcholine (ACh) induces fast inward current followed by prominent GABAergic inhibition within the sSC circuits. ACh-mediated facilitation of GABAergic inhibition may play an important role in visual signal processing in the s SC; however, both the anatomical and physiological properties of cholinergic inputs from PBN have not been studied in detail in vivo. In this thesis, I specifically visualized and optogenetically manipulated the cholinergic neurons in the PBN after focal injections of Cre-dependent viral vectors in mice that express Cre in cholinergic neurons. I revealed that the cholinergic projections terminated densely in the medial part of the mouse sSC. This suggests that the cholinergic inputs mediate visual processing in the upper visual field, which would be critical for predator detection. I further analyzed the physiological roles of the cholinergic inputs by recording looming-evoked visual responses from sSC neurons during optogenetic activation or inactivation of PBN cholinergic neurons in anesthetized mice. I found that optogenetic manipulations in either direction induced response suppression in most neurons, whereas response facilitation was observed in a few neurons after the optogenetic activation. Based on t hese results I propose a circuit model in which the PBN cholinergic inputs enhance functions of the sSC in detecting visual targets by facilitating the center excitation –surround inhibition.

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