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Augmentation of an Engineered Bacterial Strain Potentially Improves the Cleanup of PCB Water Pollution

Hara, Tomijiro Takatsuka, Yumiko Nakata, Eiji Morii, Takashi 京都大学 DOI:10.1128/spectrum.01926-21

2021.12

概要

Polychlorinated biphenyls (PCBs) are recalcitrant organohalide pollutants, consisting of 209 congeners. PCB cleanup in natural landscapes is expected to be achieved by the metabolic activity of microorganisms, but aerobic PCB-degrading bacteria that inhabit sites polluted by PCBs cannot degrade all PCB congeners due to the specificity of their enzymes. In this study, we investigated the degradability of PCBs when a genetically modified PCB-degrading bacterium was compounded with wild-type PCB-degrading bacteria. We used two bacterial strains, Comamonas testosteroni YAZ2 isolated from a PCB-uncontaminated natural landscape and Escherichia coli BL21(DE3) transformed with a biphenyl dioxygenase (BphA) gene from a well-known PCB degrader, Burkholderia xenovorans LB400. The enzymatic specificities of BphA were 2, 3-dioxygenation in the YAZ2 and 2, 3- and 3, 4-dioxygenations in the recombinant E. coli. For the PCB-degrading experiment, a dedicated bioreactor capable of generating oxygen microbubbles was prototyped and used. The combined cells of the recombinant and the wild-type strains with an appropriate composite ratio degraded 40 mg/L of Kaneclor KC-300 to 0.3 ± 0.1 mg/L within 24 h. All of the health-toxic coplanar PCB congeners in KC-300 were degraded. This study suggested that the augmentation of an engineered bacterial strain could improve the cleanup of PCB water pollution. It also revealed the importance of the ratio of the strains with different PCB-degrading profiles to efficient degradation and that the application of oxygen microbubbles could rapidly accelerate the cleanup.

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Augmentation of an Engineered Bacterial Strain Potentially Improves the Cleanup of PCB Water Pollution

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Augmentation of an Engineered Bacterial Strain Potentially Improves the Cleanup of PCB Water Pollution

概要

この論文で使われている画像

関連論文

Study on Xylan Degradation System of New Isolated Thermophilic Facultatively Anaerobic Bacterium Paenibacillus sp. Strain DA-C8

Isolation and identification of hyaluronan-degrading bacteria from Japanese fecal microbiota

Study on the characteristic properties of Gambian traditional fermented milk

Studies on Natural Products from Untapped Bacterial Resources [an abstract of entire text]

Nutrient-dependent predominant secretion of GH family enzymes by Aspergillus spp isolated from fields in subtropical region

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