リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

大学・研究所にある論文を検索できる 「Optical control of cellular signaling pathways using animal opsins」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。
リケラボ

コピーが完了しました

URLをコピーしました

論文の公開元へ論文の公開元へ
書き出し

Optical control of cellular signaling pathways using animal opsins

Tsukamoto, Hisao 塚本, 寿夫 ツカモト, ヒサオ 神戸大学

2021.10.27

概要

Currently, in various biological research fields, optical control of cellular responses is widely utilized, leading to establishing a research field named “optogenetics”. Successful optogenetic studies need both state-of-art optical devices illuminating target issues with a high spatiotemporal resolution and optical control tools (typically photosensitive proteins) driving cellular reactions in a light-dependent manner. Most of optogenetic analyses have used channelrhodopsins, lightsensitive ion channels, as control tools. Alternatively, animal opsins, light-sensitive G protein-coupled receptors, can be used as tools to drive a wide variety of G protein-dependent intracellular signaling pathways. Here, I characterize molecular properties of invertebrate opsins by spectroscopic and electrophysiological techniques, and introduce them as “ON-OFF” switch of GPCR signalings. Also, I discuss potentials of the animal opsins as optogenetic tools to expand the research field.

論文の公開元へ

関連論文

関連論文をもっと見る

参考文献

[1] Deisseroth, K., “Optogenetics.” Nat. Methods. 8(1), 26-29 (2011).

[2] Nagel, G., Szellas, T., Huhn, W., Kateriya, S., Adeishvili, N., Berthold, P., Ollig D., Hegemann, P., Bamberg, E., “Channelrhodopsin-2, a directly light-gated cation-selective membrane channel.” Proc. Natl. Acad. Sci. U S A. 100(24), 13940-13945 (2003).

[3] Airan, R. D., Thompson, K. R., Fenno, L. E., Bernstein, H., Deisseroth, K., “Temporally precise in vivo control of intracellular signalling.” Nature 458(7241), 1025-1029 (2009).

[4] Masseck, O. A., Spoida, K., Dalkara, D., Maejima, T., Rubelowski, J. M., Wallhorn, L., Deneris, E. S., Herlitze, S., “Vertebrate cone opsins enable sustained and highly sensitive rapid control of Gi/o signaling in anxiety circuitry.” Neuron 81(6), 1263-1273 (2014).

[5] Tsukamoto, H., Terakita, A., “Diversity and functional properties of bistable pigments.” Photochem. Photobiol. Sci. 9(11), 1435-1443 (2010).

[6] Arendt. D., Tessmar-Raible, K., Snyman, H., Dorresteijn, A. W., Wittbrodt, J., “Ciliary photoreceptors with a vertebrate-type opsin in an invertebrate brain.” Science. 306(5697), 869-871 (2004).

[7] Tsukamoto, H., Chen, I. S., Kubo, Y., Furutani, Y., “A ciliary opsin in the brain of a marine annelid zooplankton is ultraviolet-sensitive, and the sensitivity is tuned by a single amino acid residue.” J. Biol. Chem. 292(31), 12971- 12980 (2017).

[8] Tsukamoto, H., Furutani, Y., “Optogenetic Modulation of Ion Channels by Photoreceptive Proteins.” Adv. Exp. Med. Biol. 1293, 73-88 (2021).

参考文献をもっと見る