The anti-inflammatory effect of geniposidic acid to Porphyromonas gingivalis-induced periodontitis

概要

封‫੽ކ‬伏
The anti-inflammatory effect of geniposidic acid to
Porphyromonas gingivalis-induced periodontitis
ͧPorphyromonas gingivalis ࡟ࡼࡗ࡚ㄏⓎࡉࢀࡿṑ࿘⅖
࡟ᑐࡍࡿࢤࢽ࣏ࢩࢻ㓟ࡢᢠ⅖⑕స⏝㸧

=+$,5824,

Objective
Periodontal disease is a chronic disease that causes damage of the gums.
The major pathogenic bacteria is Porphyromonas gingivalis, a gramnegative bacterium produces variety of pathogenic factors to induce
inflammation in host. Eucommia ulmoides is a plant from China, which
has the effects of reducing blood pressure, weight loss and antiinflammatory. Geniposidic acid (GPA) is the major component of
Eucommia ulmoides. This study was to investigate the effect of GPA on P.
gingivalis induced periodontitis.
Materials ͡ Methods
In order to investigate the anti-inflammatory effect of GPA, the IL-6
mRNA expression by real-time-PCR and production of IL-6 by ELISA in
gingival epithelial cell (HGEC) with or without P. gingivalis stimulation

and GPA addition were determined. The suppressive effect of GPA in P.
gingivalis-induced mouse periodontitis model was analyzed.
Results
The addition of GPA showed the suppressive effect of IL-6 mRNA
induction and IL-6 production in HGEC stimulated by P. gingivalis. GPA
also suppressed P. gingivalis-induced TLR2 induction in HGEC. The
inoculation of GPA showed the inhibitory effect of P. gingivalis-induced
alveolar bone resorption in mouse via suppressing IL-6 production in
serum and gingival tissue. Furthermore, osteoclast differentiation of bone
marrow cells by M-CSF and sRANKL of mouse was suppressed in the
presence of GPA.
Conclusion
GPA had the anti-inflammatory effect in periodontal tissue and showed to
be effective in preventing periodontal disease.