The anti-inflammatory effect of geniposidic acid to Porphyromonas gingivalis-induced periodontitis

概要

Periodontal disease (PD) is the inflammatory condition caused by the interaction between periodontopathogenic bacteria and immune response. Porphyromonas gingivalis, the major pathogenic bacteria and a gram-negative bacterium produces variety of pathogenic factors to induce inflammation in host. Geniposidic acid (GPA) is the major component of Eucommia ulmoides, has various pharmacological effects (anti-inflammation, anti-angiocardiopathy and anti-neurodegeneration). However，the effect and mechanism of GPA on Porphyromonas gingivalis induced periodontitis have not been clear. Therefore, in this study, we investigated the effect of GPA on Pg infection in front-line gingival epithelial cells against periodontopathic bacteria and the effect of GPA on a mouse periodontitis model.

　To determine the anti-inflammatory effect of GPA, the mRNA expression of IL-6 in gingival epithelial cell (HGEC) with or without P.gingivalis stimulation and Ga addition. The suppressive effect of GPA in P.gingivalis-induced mouse periodontitis model was analyzed.

　The results showed the addition of GPA inhibited the production of IL-6 following the TLR2 in HGEC. GPA also showed the inhibitory effect of P. gingivalis-induced alveolar bone resorption in mouse via suppressing IL-6 production in serum and gingival tissue. In conclusion, the above results show that GPA had the anti-inflammatory effect in periodontal tissue and showed to be effective in preventing periodontal disease.

Keywords : Porphyromonas gingivalis, geniposidic acid, bone resorption, IL-6