Conserved Double Translation Initiation Site for Δ160p53 Protein Hints at Isoform’s Key Role in Mammalian Physiology

1.

2.

3.

4.

5.

6.

7.

8.

Lane, D.P.; Cheok, C.F.; Brown, C.; Madhumalar, A.; Ghadessy, F.J.; Verma, C. Mdm2 and p53 are highly conserved from

placozoans to man. Cell Cycle 2010, 9, 540–547. [CrossRef] [PubMed]

Donehower, L.A.; Harvey, M.; Slagle, B.L.; McArthur, M.J.; Montgomery, C.A.; Butel, J.S.; Bradley, A. Mice deficient for p53 are

developmentally normal but susceptible to spontaneous tumours. Nature 1992, 356, 215–221. [CrossRef] [PubMed]

Maier, B.; Gluba, W.; Bernier, B.; Turner, T.; Mohammad, K.; Guise, T.; Sutherland, A.; Thorner, M.; Scrable, H. Modulation of

mammalian life span by the short isoform of p53. Genes Dev. 2004, 18, 306–319. [CrossRef]

Anbarasan, T.; Bourdon, J.-C. The Emerging Landscape of p53 Isoforms in Physiology, Cancer and Degenerative Diseases. Int. J.

Mol. Sci. 2019, 20, 6257. [CrossRef] [PubMed]

Chen, P.L.; Chen, Y.M.; Bookstein, R.; Lee, W.H. Genetic mechanisms of tumor suppression by the human p53 gene. Science 1990,

250, 1576–1580. [CrossRef] [PubMed]

Bourougaa, K.; Naski, N.; Boularan, C.; Mlynarczyk, C.; Candeias, M.M.; Marullo, S.; Fahraeus, R. Endoplasmic reticulum stress

induces G2 cell-cycle arrest via mRNA translation of the p53 isoform p53/47. Mol. Cell 2010, 38, 78–88. [CrossRef] [PubMed]

Candeias, M.M.; Malbert-Colas, L.; Powell, D.J.; Daskalogianni, C.; Maslon, M.M.; Naski, N.; Bourougaa, K.; Calvo, F.; Fahraeus,

R. P53 mRNA controls p53 activity by managing Mdm2 functions. Nat. Cell Biol. 2008, 10, 1098–1105. [CrossRef] [PubMed]

Candeias, M.M.; Hagiwara, M.; Matsuda, M. Cancer-specific mutations in p53 induce the translation of ∆160p53 promoting

tumorigenesis. EMBO Rep. 2016, 17, 1542–1551. [CrossRef] [PubMed]

Int. J. Mol. Sci. 2022, 23, 15844

9.

10.

11.

12.

13.

14.

15.

16.

17.

18.

19.

11 of 11

Niu, G.; Hellmuth, I.; Flisikowska, T.; Pausch, H.; Rieblinger, B.; Carrapeiro, A.; Schade, B.; Böhm, B.; Kappe, E.; Fischer, K.;

et al. Porcine model elucidates function of p53 isoform in carcinogenesis and reveals novel circTP53 RNA. Oncogene 2021, 40,

1896–1908. [CrossRef] [PubMed]

Candeias, M.M. Mutant p53 loses “gain-of-functions” after losing isoform expression. In Proceedings of the 6th International

Mutant p53 Workshop; Cell Death and Differentiation Conferences, Toronto, ON, USA, 12–14 September 2013; p. 1.

Lacerda, R.; Menezes, J.; Candeias, M.M. Alternative Mechanisms of mRNA Translation Initiation in Cellular Stress Response and

Cancer. In The mRNA Metabolism in Human Disease; Springer: Berlin/Heidelberg, Germany, 2019; pp. 117–132.

Sachs, M.S.; Wang, Z.; Gaba, A.; Fang, P.; Belk, J.; Ganesan, R.; Amrani, N.; Jacobson, A. Toeprint analysis of the positioning of

translation apparatus components at initiation and termination codons of fungal mRNAs. Methods 2002, 26, 105–114. [CrossRef]

[PubMed]

Levav-Cohen, Y.; Goldberg, Z.; Tan, K.H.; Alsheich-Bartok, O.; Zuckerman, V.; Haupt, S.; Haupt, Y. The p53-Mdm2 loop: A critical

juncture of stress response. Subcell. Biochem. 2014, 85, 161–186. [PubMed]

Marques-Ramos, A.; Candeias, M.M.; Menezes, J.; Lacerda, R.; Willcocks, M.; Teixeira, A.; Locker, N.; Romão, L. Cap-independent

translation ensures mTOR expression and function upon protein synthesis inhibition. RNA 2017, 23, 1712–1728. [CrossRef]

[PubMed]

Bazykin, G.A.; Kochetov, A.V. Alternative translation start sites are conserved in eukaryotic genomes. Nucleic Acids Res. 2011,

39, 567–577. [CrossRef] [PubMed]

Candeias, M.M.; Powell, D.J.; Roubalova, E.; Apcher, S.; Bourougaa, K.; Vojtesek, B.; Bruzzoni-Giovanelli, H.; Fahraeus, R.

Expression of p53 and p53/47 are controlled by alternative mechanisms of messenger RNA translation initiation. Oncogene 2006,

25, 6936–6947. [CrossRef] [PubMed]

Midgley, C.A.; Fisher, C.J.; Bartek, J.; Vojtesek, B.; Lane, D.; Barnes, D.M. Analysis of p53 expression in human tumours: An

antibody raised against human p53 expressed in Escherichia coli. J. Cell Sci. 1992, 101, 183–189. [CrossRef] [PubMed]

Katoh, K. MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res.

2002, 30, 3059–3066. [CrossRef] [PubMed]

Gould, P.S.; Bird, H.; Easton, A.J. Translation toeprinting assays using fluorescently labeled primers and capillary electrophoresis.

Biotechniques 2005, 38, 397–400. [CrossRef] [PubMed]

...