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T-DNA Tagging-Based Gain-of-Function of OsHKT1;4 Reinforces Na Exclusion from Leaves and Stems but Triggers Na Toxicity in Roots of Rice Under Salt Stress

Oda, Yuuka Kobayashi, Natsuko I. Tanoi, Keitaro Ma, Jian Feng Itou, Yukiko Katsuhara, Maki Itou, Takashi Horie, Tomoaki 信州大学 DOI:29329278

2020.02.28

概要

The high affinity K+ transporter 1;4 (HKT1;4) in rice (Oryza sativa), which shows Na+ selective transport with little K+ transport activity, has been suggested to be involved in reducing Na in leaves and stems under salt stress. However, detailed physiological roles of OsHKT1;4 remain unknown. Here, we have characterized a transfer DNA (T-DNA) insertion mutant line of rice, which overexpresses OsHKT1;4, owing to enhancer elements in the T-DNA, to gain an insight into the impact of OsHKT1;4 on salt tolerance of rice. The homozygous mutant (the O/E line) accumulated significantly lower concentrations of Na in young leaves, stems, and seeds than the sibling WT line under salt stress. Interestingly, however, the mutation rendered the O/E plants more salt sensitive than WT plants. Together with the evaluation of biomass of rice lines, rhizosphere acidification assays using a pH indicator bromocresol purple and (NaCl)-Na-22 tracer experiments have led to an assumption that roots of O/E plants suffered heavier damages from Na which excessively accumulated in the root due to increased activity of Na+ uptake and Na+ exclusion in the vasculature. Implications toward the application of the HKT1-mediated Na+ exclusion system to the breeding of salt tolerant crop cultivars will be discussed.

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T-DNA Tagging-Based Gain-of-Function of OsHKT1;4 Reinforces Na Exclusion from Leaves and Stems but Triggers Na Toxicity in Roots of Rice Under Salt Stress

概要

関連論文

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大学

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T-DNA Tagging-Based Gain-of-Function of OsHKT1;4 Reinforces Na Exclusion from Leaves and Stems but Triggers Na Toxicity in Roots of Rice Under Salt Stress

概要

関連論文

Physiological and molecular mechanisms of salt removal by leaf sheath in rice

Effects of improved sodium uptake ability on grain yields of rice plants under low potassium supply

Physiological and Genetic Mechanisms of Lateral Root Formation for the Improvement of Root System Architecture in Rice

Physiological and genetic study on mechanisms of growth promotion and salt tolerance improvement in rice seedlings by inoculation with biofertilizer microorganism Bacillus pumilus TUAT1 strain

A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1

参考文献