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The usefulness of re-attachability of anti-adhesive cross-linked gelatin film and the required physical and biological properties.

HORII Tsunehito 70838458 0000-0002-5348-856X TSUJIMOTO Hiroyuki KAGEYAMA Susumu 50378452 0000-0001-7150-647X YOSHIDA Tetsuya 60510310 KOBAYASHI Kenichi 40727434 TAKAMORI Hideki MINATO Hiroshi UEDA Jo HAGIWARA Akeo ICHIKAWA Hiroshi KAWAUCHI Akihiro 90240952 滋賀医科大学

2020.12.11

概要

Background:
To overcome the unfavorable issues associated with conventional anti-adhesive HA/CMC film, we developed an anti-adhesive thermally cross-linked gelatin film.
Objective:
We tried to clarify the re-attachability of the film and the required properties concerning the film thickness, stiffness and anti-adhesion effect.
Methods:
To determine the optimal thickness, 5 kinds of the thickness of gelatin film and the conventional film were analyzed by the tensile test, shearing test, buckling test and tissue injury test. Finally, using the optimal film thickness, we tried to clarify the anti-adhesion effect of the reattached film.
Results:
The tensile and shearing test showed gelatin films ≥30 μm thick had greater tensile strength and a smaller number of film fractures, than the conventional film. The buckling and tissue injury test showed gelatin films ≥60 μm thick had higher buckling strength and worse injury scores than the conventional film. The anti-adhesive effect of re-attached gelatin film using optimal thickness (30-40 μm) found the anti-adhesion score was significantly better than that of the control.
Conclusions:
Provided it has an optimal thickness, gelatin film can be reattached with enough physical strength not to tear, safety stiffness not to induce tissue injury, and a sufficient anti-adhesion effect.

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参考文献

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Tables

TABLE 1. Tissue Injury Scores

Description

Score

Minor (No Injury)

Moderate (A little hemorrhage with small tissue injury)

Serious (Middle hemorrhage with tissue injury)

Severe (Hemorrhage with sharp tissue injury)

TABLE 2. The Adhesion Scores

Category and Description

Score

(Extent)

No Involvment

<25% of the site involved

<50% of the site involved

<75% of the site involved

<100% of the site involved

(Severity)

No adhesion present

Adhesions fall apart

Adhesions can be lysed with traction

Adhesions requiring <50% sharp dissection

Adhesions requiring <50% sharp dissection

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Figure captions

FIGURE.1

The schematic illustrations of Tensile test (a), Buckling test (b) and Shearing test (c).

FIGURE.2

(a): The maximum tensile loads of each thickness of gelatin film and the conventional film. (b):

The maximum buckling loads of each thickness of gelatin film and the conventional film.

Statistically significant relative to conventional film (*:p<0.05, **:p<0.01)

FIGURE.3

(a): The fracture number of each thickness of gelatin film and the conventional film in shearing

test. (b): The tissue injury scores of of each thickness of gelatin film and the conventional film.

Statistically significant relative to conventional film (**:p<0.01)

FIGURE.4

The anti-adhesion scores of anti-adhesion effect test with 30 μm thickness of gelatin film, reattached gelatin film and the conventional film. Statistically significant relative to control

(*:p<0.05)

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Figures

FIGURE.1

FIGURE.2

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FIGURE.3

FIGURE.4

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