Effects of high-frequency near-infrared diode laser irradiation on pain induced by experimental tooth movement in rats

概要

ㄽ ᩥ ඲ ᩥ せ ⣙
Effects of high-frequency near-infrared diode laser
irradiation on pain induced by experimental tooth
movement in rats
㸦㧗࿘Ἴ㏆㉥እ⥺༙ᑟయ࣮ࣞࢨ࣮↷ᑕࡀࣛࢵࢺࢆ⏝࠸ࡓᐇ㦂ⓗṑࡢ
⛣ືࣔࢹࣝ࡟࠾ࡅࡿ⑊③࡟୚࠼ࡿᙳ㡪㸧 

୺ᣦᑟᩍဨ㸸㇂ᮏ ᖾኴ㑻 ᩍᤵ
㸦་⣔⛉Ꮫ◊✲⛉ ṑ⛉▹ṇᏛ㸧
๪ᣦᑟᩍဨ㸸ᰠᮏ ┤ஓ ᩍᤵ
㸦་⣔⛉Ꮫ◊✲⛉ ṑ⛉ᨺᑕ⥺Ꮫ㸧
๪ᣦᑟᩍဨ㸸ᅧᯇ  ு ㅮᖌ
㸦་⣔⛉Ꮫ◊✲⛉ ṑ⛉▹ṇᏛ㸧

୰㇂ ᩥ㤶
㸦་ṑ⸆ಖ೺Ꮫ◊✲⛉ ་ṑ⸆Ꮫᑓᨷ㸧

ㄽᩥ඲ᩥせ⣙
▹ṇṑ⛉἞⒪ࡣࠊ୙ṇညྜᝈ⪅࡟ᑐࡋ㢡㢦㠃ཱྀ࣭⭍⤌⧊ࡢⓎ⫱ࢆㄏᑟࡋࠊṇᖖ࡞ညྜᶵ⬟ࢆᵓ⠏ࡍࡿ་⒪࡛
࠶ࡿࠋ἞⒪୰࡟࠾ࡅࡿṑࡢ⛣ືࡣࠊᶵᲔⓗ࡞ຊ (▹ṇຊ) ࢆ௜୚ࡍࡿࡇ࡜࡟ࡼࡾࠊᅽ㏕ഃ࡛ࡢ㦵྾཰࡜≌ᘬഃ
࡛ࡢ㦵ῧຍࡢ㦵ᨵ㐀⌧㇟࡟ࡼࡗ࡚㐩ᡂࡉࢀࡿࠋࡋ࠿ࡋ࡞ࡀࡽࠊ▹ṇຊࡀ㢡㦵࠶ࡿ࠸ࡣṑ࡟௜୚ࡍࡿࡇ࡜࡟ࡣࠊ
ࡋࡤࡋࡤ⑊③ࡀక࠺ࠋࡶࡋࠊṑࡢ⛣ື࡟ᑐࡍࡿ⑊③⦆࿴ฎ⨨ࡀ☜❧ࡉࢀࢀࡤࠊࡼࡾᝈ⪅㈇ᢸࡢᑡ࡞࠸἞⒪ࡀྍ
⬟࡜࡞ࡿࠋ࣮ࣞࢨ࣮ࡣࠊບ㉳≧ែ࡟࠶ࡿ≀㉁ࡢㄏᑟᨺᑕ࡟ࡼࡾⓎ⏕ࡍࡿ≉ᐃࡢ㟁☢Ἴ࡛࠶ࡾࠊ་⛉࣭ṑ⛉㡿ᇦ
࡟࠾࠸࡚ࠊ࣮ࣞࢨ࣮↷ᑕࡢ⏕య࡟ᑐࡍࡿග่⃭స⏝ࡀᗈࡃᛂ⏝ࡉࢀ࡚࠸ࡿࠋ㏆ᖺࠊࢼࣀࢭ࢝ࣥࢻ༢఩ࡢࣃࣝࢫ
Ἴࢆ↷ᑕ࡛ࡁࡿ㧗࿘Ἴ㏆㉥እ⥺༙ᑟయ࣮ࣞࢨ࣮ (NIR ࣮ࣞࢨ࣮) ࡀ㛤Ⓨࡉࢀࠊ⤌⧊ෆ࡟⇕ᦆയࢆཬࡰࡍࡇ࡜࡞
ࡃࠊග࢚ࢿࣝࢠ࣮ࢆࡼࡾຠ⋡ⓗ࡟⤌⧊ෆ࡬ᾐ㏱ࡉࡏࡿࡇ࡜ࡀྍ⬟࡜࡞ࡗࡓࠋṑࡢ⛣ື࡟ࡼࡗ࡚ㄏⓎࡉࢀࡓ⑊③
࡟ᑐࡍࡿ NIR ࣮ࣞࢨ࣮↷ᑕࡢᙳ㡪࡟ࡘ࠸࡚ࠊヲ⣽࡞᳨ウࢆ⾜ࡗࡓሗ࿌ࡣᑡ࡞࠸ࠋᮏ◊✲࡛ࡣࠊNIR ࣮ࣞࢨ࣮
↷ᑕࡀᐇ㦂ⓗ࡞ṑࡢ⛣ື࡟ࡼࡗ࡚ㄏⓎࡉࢀࡓ⑊③࡬ཬࡰࡍᙳ㡪࡟ࡘ࠸࡚ࠊ⤌⧊Ꮫⓗ࡟᫂ࡽ࠿࡟ࡍࡿࡇ࡜࡜ࡋࡓࠋ
ࡲࡎࠊࣛࢵࢺࡢୖ㢡ᕥഃ➨୍⮻ṑ࠿ࡽୖ㢡㛛ṑࡢ㛫࡟ Ni-Ti ࢡ࣮ࣟࢬࢻࢥ࢖ࣝࢆ⿦╔ࡋ 50 g ࡢ▹ṇຊࢆ௜
୚ࡋࠊࡇࢀࢆᐇ㦂ⓗṑࡢ⛣ື (ETM) ࣔࢹࣝ࡜ࡋࡓࠋ࣮ࣞࢨ࣮↷ᑕࡣࠊἼ㛗 910 nm ࡢ㉸▷ࣃࣝࢫ࡛ࠊ➨୍⮻
ṑ࡟ྜィ 120 J ࡢ࢚ࢿࣝࢠ࣮㔞ࢆ↷ᑕࡋࡓࠋETM ฎ⨨ᚋࠊ࣮ࣞࢨ࣮↷ᑕࢆ⾜ࡗࡓ⩌ࢆ ETM+࣮ࣞࢨ࣮ฎ⨨⩌
࡜ࡋࠊETM ⿦⨨ࢆ⿦╔ࡋ࡚࠸࡞࠸ྑഃࢆᑐ↷⩌࡜ࡋࡓࠋ౵ᐖཷᐜ⾜ືヨ㦂࡛ࡣࠊYang ࡽࡢ᪉ἲ࡟‽ࡌ࡚ࠊࣇ
࢙࢖ࢩࣕࣝࢢ࣮࣑ࣝࣥࢢ㐠ືࡢᅇᩘ࡜࿪ᄮ㐠ື᫬㛫㸦VCM㸧ࢆホ౯ࡋࡓࠋḟ࡟ࠊNIR ࣮ࣞࢨ࣮ࡀᐇ㦂ⓗṑࡢ
⛣ື᫬࡟ཱྀ⭍ෆ③ぬࢆྖࡿ⚄⤒⥺⥔ࡢᢞᑕࡍࡿ୕ཫ⚄⤒⬨㧊㊰᰾ᑿഃள᰾㸦Vc㸧࡟ཬࡰࡍᙳ㡪࡟ࡘ࠸᳨࡚ウࢆ
⾜ࡗࡓࠋ⿦⨨⿦╔࠾ࡼࡧ࣮ࣞࢨ࣮↷ᑕࡢ 24 ᫬㛫ᚋ࡟ࣛࢵࢺࢆ℺ὶᅛᐃࡋࡓࠋ⚄⤒⯆ዧࡢᣦᶆ࡜ࡋ࡚ c-Fos ࢱ
ࣥࣃࢡ㉁ᵝච␿཯ᛂᛶࢽ࣮ࣗࣟࣥ㸦Fos-IR㸧ࢆච␿⤌⧊ᏛᰁⰍ࡟᳨࡚ウࡋࡓࠋࡲࡓࠊ࣑ࢡࣟࢢࣜ࢔ࡢᣦᶆ࡛࠶
ࡿ Iba-1ࠊ࠾ࡼࡧ࢔ࢫࢺࣟࢧ࢖ࢺࡢᣦᶆ࡛࠶ࡿ GFAP ࡟ࡘ࠸࡚⺯ගච␿⤌⧊ᏛᰁⰍࢆ⾜ࡗࡓࠋVc I/II ᒙ㡿ᇦ
࡟࠾ࡅࡿ Fos-IR 㝧ᛶࢽ࣮ࣗࣟࣥᩘࢆࠊIba-1 ࠾ࡼࡧ GFAP ࡢ㝧ᛶ⣽⬊㠃✚⋡ࢆᐃ㔞ホ౯ࡋࡓࠋ᭱ᚋ࡟ࠊNIR
࣮ࣞࢨ࣮ࡀᐇ㦂ⓗṑࡢ⛣ື᫬࡟࠾ࡅࡿṑ࿘⤌⧊࡟ཬࡰࡍᙳ㡪࡟ࡘ࠸᳨࡚ウࢆ⾜ࡗࡓࠋ⿦⨨⿦╔࠾ࡼࡧ࣮ࣞࢨ࣮
↷ᑕࡢ 24 ᫬㛫ᚋ࡟ࣛࢵࢺࢆ℺ὶᅛᐃࡋࠊୖ㢡㦵ࢆ᦬ฟࡋࠊ➨୍⮻ṑࡢ㏆ᚰ᰿ᅽ㏕ഃṑ᰿⭷㡿ᇦ࡟࠾ࡅࡿ࢖ࣥ
ࢱ࣮ࣟ࢖࢟ࣥ㸦IL㸧-1βࠊࢩࢡࣟ࢜࢟ࢩࢤࢼ࣮ࢮ㸦COX㸧-2ࠊࣉࣟࢫࢱࢢࣛࣥࢪࣥ㸦PG㸧E-2ࠊ࠾ࡼࡧ࢝ࣝࢩ
ࢺࢽࣥ㑇ఏᏊ㛵㐃࣌ࣉࢳࢻ㸦CGRP㸧ࡢࢱࣥࣃࢡ㉁Ⓨ⌧࡟ࡘ࠸࡚ච␿⤌⧊Ꮫⓗ᳨ウࢆ⾜ࡗࡓࠋ௨ୗࡢ⤖ᯝࡀ᫂
ࡽ࠿࡜࡞ࡗࡓࠋ
1. ETM ࡟ࡼࡗ࡚ቑຍࡋࡓࣇ࢙࢖ࢩࣕࣝࢢ࣮࣑ࣝࣥࢢᅇᩘ࠾ࡼࡧ VCM ᫬㛫ࡣࠊNIR ࣮ࣞࢨ࣮↷ᑕ࡟ࡼࡗ
࡚᭷ព࡟ῶᑡࡋࡓࠋࡋࡓࡀࡗ࡚ࠊETM ࡟ࡼࡗ࡚ቑຍࡋࡓ౵ᐖཷᐜ⾜ືࡣ NIR ࣮ࣞࢨ࣮࡟ࡼࡗ࡚ῶᑡࡍ
ࡿࡇ࡜ࡀ♧ࡉࢀࡓࠋ
2. ETM ࡟ࡼࡗ࡚ቑຍࡋࡓ Vc I/II ࡟࠾ࡅࡿ Fos-IR 㝧ᛶ⣽⬊ᩘࡣࠊNIR ࣮ࣞࢨ࣮↷ᑕ࡟ࡼࡗ࡚᭷ព࡟ῶᑡ
ࡋࡓࠋETM ࡟ࡼࡗ࡚ Iba-1 ࠾ࡼࡧ GFAP ࡢ㝧ᛶ㠃✚⋡ࡢ᭷ព࡞ஹ㐍ࢆㄆࡵࠊ࣮ࣞࢨ࣮↷ᑕ࡟ࡼࡗ࡚᭷
ព࡟ᢚไࡉࢀࡓࠋࡋࡓࡀࡗ࡚ NIR ࣮ࣞࢨ࣮↷ᑕࡣ୕ཫ⚄⤒⬨㧊㊰᰾ᑿഃள᰾࡟࠾࠸࡚ࠊ࣑ࢡࣟࢢࣜ࢔
࠾ࡼࡧ࢔ࢫࢺࣟࢧ࢖ࢺࡢάᛶࢆᢚไࡍࡿࡇ࡜ࡀ♧၀ࡉࢀࡓࠋ
3. ETM ࡟ࡼࡗ࡚ቑຍࡋࡓ➨୍⮻ṑࡢ㏆ᚰ᰿ᅽ㏕ഃṑ᰿⭷㡿ᇦ࡟࠾ࡅࡿ IL-1βࠊPGE2ࠊCOX2ࠊ࠾ࡼࡧ
CGRP ࡢࢱࣥࣃࢡ㉁Ⓨ⌧ࡣࠊ࣮ࣞࢨ࣮↷ᑕ࡟ࡼࡗ࡚ᢚไࡉࢀࡓࠋࡋࡓࡀࡗ࡚ࠊNIR ࣮ࣞࢨ࣮↷ᑕࡣᅽ㏕
ഃṑ᰿⭷㡿ᇦ࡟࠾࠸࡚ࠊETM ࡟ࡼࡗ࡚ㄏⓎࡉࢀࡓ⅖⑕ᛶ࣐࣮࣮࢝ࡢⓎ⌧ࢆῶᑡࡉࡏࡿࡇ࡜ࡀ♧ࡉࢀࡓࠋ
ᮏ◊✲ࡼࡾࠊNIR ࣮ࣞࢨ࣮↷ᑕṑࡢ⛣ື᫬࡟ㄏⓎࡉࢀࡿ⑊③ࡀ㍍ῶࡉࢀࡿྍ⬟ᛶࡀ♧၀ࡉࢀࡓࠋ