リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

大学・研究所にある論文を検索できる 「The effect of gemcitabine on cell cycle arrest and microRNA signatures in pancreatic cancer cells」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

コピーが完了しました

URLをコピーしました

論文の公開元へ論文の公開元へ
書き出し

The effect of gemcitabine on cell cycle arrest and microRNA signatures in pancreatic cancer cells

波間 大輔 香川大学 DOI:10.21873/invivo.12155

2020.12.24

概要

Background/aim: Gemcitabine, an inhibitor of DNA synthesis, is the gold standard chemotherapeutic agent for pancreatic ductal adenocarcinoma (PDAC). MicroRNAs (miRNAs) play critical roles in cancers, including PDAC. However, less is known about the effect of gemcitabine on PDAC cells and miRNA expression in PDAC. We evaluated the effect of gemcitabine on the cell cycle of PDAC cells in vitro and in vivo and on the miRNA expression profile.
Materials and methods: Effects of gemcitabine on PK-1 and PK-9 cell growth were evaluated using a cell counting kit-8 assay. Xenografted mouse models were used to assess gemcitabine effects in vivo.
Results: Gemcitabine inhibited the proliferation and tumour growth of PK-1 cells, and induced S phase cell cycle arrest. Numerous miRNAs were altered upon gemcitabine treatment of PK-1 cells and xenograft models.
Conclusion: Altered miRNAs may serve as potential therapeutic targets for improving the efficacy of gemcitabine in PDAC.

この論文で使われている画像

参考文献

1 GBD 2017 Pancreatic Cancer Collaborators: The global,

regional, and national burden of pancreatic cancer and its

attributable risk factors in 195 countries and territories, 19902017: a systematic analysis for the Global Burden of Disease

Study 2017. Lancet Gastroenterol Hepatol 4(12): 934-947, 2019.

PMID: 31648972. DOI: 10.1016/S2468-1253(19)30347-4

2 Howes N, Lerch MM, Greenhalf W, Stocken DD, Ellis I, Simon

P, Truninger K, Ammann R, Cavallini G, Charnley RM, Uomo

G, Delhaye M, Spicak J, Drumm B, Jansen J, Mountford R,

Whitcomb DC and Neoptolemos JP: Clinical and genetic

characteristics of hereditary pancreatitis in Europe. Clin

Gastroenterol Hepatol 2(3): 252, 2004. PMID: 15017610. DOI:

10.1016/s1542-3565(04)00013-8

3 Kamisawa T, Wood LD, Itoi T and Takaori K: Pancreatic cancer.

Lancet 388(10039): 73-85, 2016. PMID: 26830752. DOI:

10.1016/S0140-6736(16)00141-0

4 Neoptolemos JP, Stocken DD, Friess H, Bassi C, Dunn JA,

Hickey H, Beger H, Fernandez-Cruz L, Dervenis C, Lacaine F,

Falconi M, Pederzoli P, Pap A, Spooner D, Kerr DJ, Büchler

MW and European Study Group for Pancreatic Cancer: A

randomized trial of chemoradiotherapy and chemotherapy after

resection of pancreatic cancer. N Engl J Med 350: 1200-1210,

2004. PMID: 15028824. DOI: 10.1056/NEJMoa032295

5 Vaccaro V, Sperduti I, Vari S, Bria E, Melisi D, Garufi C, Nuzzo

C, Scarpa A, Tortora G, Cognetti F, Reni M and Milella M:

Metastatic pancreatic cancer: Is there a light at the end of the

tunnel? World J Gastroenterol 21(16): 4788-4801, 2015. PMID:

25944992. DOI: 10.3748/wjg.v21.i16.4788

6 Burris HA III, Moore MJ, Andersen J, Green MR, Rothenberg

ML, Modiano MR, Cripps MC, Portenoy RK, Storniolo AM,

Tarassoff P, Nelson R, Dorr FA, Stephens CD and Von Hoff DD:

Improvements in survival and clinical benefit with gemcitabine

as first-line therapy for patients with advanced pancreas cancer:

a randomized trial. J Clin Oncol 15: 2403-2413, 1997. PMID:

9196156. DOI: 10.1200/JCO.1997.15.6.2403

7 Oettle H, Post S, Neuhaus P, Gellert K, Langrehr J, Ridwelski

K, Schramm H, Fahlke J, Zuelke C, Burkart C, Gutberlet K,

Kettner E, Schmalenberg H, Weigang-Koehler K, Bechstein

WO, Niedergethmann M, Schmidt-Wolf I, Roll L, Doerken B

and Riess H: Adjuvant chemotherapy with gemcitabine vs

observation in patients undergoing curative-intent resection of

pancreatic cancer: a randomized controlled trial. JAMA 297:

267-277, 2007. PMID: 17227978. DOI: 10.1001/jama.297.3.267

8 Pasquinelli AE: MicroRNAs and their targets: recognition,

regulation and an emerging reciprocal relationship. Nat Rev Genet

13: 271-282, 2012. PMID: 22411466. DOI: 10.1038/nrg3162

9 Kozinn SI, Harty NJ, Delong JM, Deliyiannis C, Logvinenko T,

Summerhayes IC, Libertino JA, Holway AH and Rieger-Christ

KM: MicroRNA profile to predict gemcitabine resistance in

bladder carcinoma cell lines. Genes Cancer 4: 61-69, 2013.

PMID: 23946872. DOI: 10.1177/1947601913484495

10 Li H and Yang BB: Friend or foe: the role of microRNA in

chemotherapy resistance. Acta Pharmacol Sin 34: 870-879,

2013. PMID: 23624759. DOI: 10.1038/aps.2013.35

11 Kawaguchi T, Komatsu S, Ichikawa D, Morimura R, Tsujiura M,

Konishi H, Takeshita H, Nagata H, Arita T, Hirajima S, Shiozaki

A, Ikoma H, Okamoto K, Ochiai T, Taniguchi H and Otsuji E:

Clinical impact of circulating miR-221 in plasma of patients

3202

12

13

14

15

16

17

18

19

20

21

22

23

with pancreatic cancer. Br J Cancer 108: 361-369, 2013. PMID:

23329235. DOI: 10.1038/bjc.2012.546

Zhao G, Zhang JG, Liu Y, Qin Q, Wang B, Tian K, Liu L, Li X,

Niu Y, Deng SC and Wang CY: miR-148b functions as a tumor

suppressor in pancreatic cancer by targeting AMPKα1. Mol

Cancer Ther 12: 83-93, 2013. PMID: 23171948. DOI: 10.1158/

1535-7163.MCT-12-0534-T

Chen WY, Liu WJ, Zhao YP, Zhou L, Zhang TP, Chen G and

Shu H: Induction, modulation and potential targets of miR-210

in pancreatic cancer cells. Hepatobiliary Pancreat Dis Int 11:

319-324, 2012. PMID: 22672828. DOI: 10.1016/s14993872(12)60168-4

Toyota Y, Iwama H, Kato K, Tani J, Katsura A, Miyata M,

Fujiwara S, Fujita K, Sakamoto T, Fujimori T, Okura R,

Kobayashi K, Tadokoro T, Mimura S, Nomura T, Miyoshi H,

Morishita A, Kamada H, Yoneyama H, Okano K, Suzuki Y and

Masaki T: Mechanism of gemcitabine-induced suppression of

human cholangiocellular carcinoma cell growth. Int J Oncol 47(4):

1293-1302, 2015. PMID: 26252371. DOI: 10.3892/ijo.2015.3118

Miyata M, Morishita A, Sakamoto T, Katsura A, Kato K,

Nishioka T, Toyota Y, Fujita K, Maeda E, Nomura T, Tani J,

Miyoshi H, Yoneyama H, Kobara H, Fujiwara S, Nishiyama N,

Iwama H, Himoto T, Hirashima M and Masaki T: MicroRNA

profiles in cisplatin-induced apoptosis of hepatocellular

carcinoma cells. Int J Oncol 47(2): 535-542, 2015. PMID:

26060089. DOI: 10.3892/ijo.2015.3036

Calabretta S, Bielli P, Passacantilli I, Pilozzi E, Fendrich V,

Capurso G, Fave GD and Sette C: Modulation of PKM

alternative splicing by PTBP1 promotes gemcitabine resistance

in pancreatic cancer cells. Oncogene 35: 2031-2039, 2016.

PMID: 26234680. DOI: 10.1038/onc.2015.270

Binenbaum Y, Na’ara S and Gil Z: Gemcitabine resistance in

pancreatic ductal adenocarcinoma. Drug Resist Updat 23: 55-68,

2015. PMID: 26690340. DOI: 10.1016/j.drup.2015.10.002

Hui YF and Reitz J: Gemcitabine: a cytidine analogue active

against solid tumors. Am J Health Syst Pharm 54: 162-170,

1997. PMID: 9117804. DOI: 10.1093/ajhp/54.2.162

Carpenelli G, Bucci G, D’Agnano I, Canese R, Caroli F, Raus

L, Brunetti E, Giannarelli E, Podo F and Carapella CM:

Gemcitabine treatment of experimental C6 glioma: the effects

on cell cycle and apoptotic rate. Anticancer Res 26: 3017-3024,

2006. PMID: 16886629.

Zhang C, Li H, Wang J, Zhang J and Hou X: MicroRNA-3383p suppresses cell proliferation, migration and invasion in

human malignant melanoma by targeting MACC1. Exp Ther

Med 18(2): 997-1004, 2019. PMID: 31316597. DOI:

10.3892/etm.2019.7644

Y Galmarini CM, Clarke ML, Falette N, Puisieux A, Mackey JR

and Dumontet C: Expression of a non-functional p53 affects the

sensitivity of cancer cells to gemcitabine. Int J Cancer 97(4):

439-445, 2002. PMID: 11802204. DOI: 10.1002/ijc.1628

Morgan MA, Parsels LA, Parsels JD, Mesiwala AK, Maybaum

J and Lawrence TS: Role of checkpoint kinase 1 in preventing

premature mitosis in response to gemcitabine. Cancer Res 65:

6835-6842, 2005. PMID: 16061666. DOI: 10.1158/0008-5472.

CAN-04-2246

Cappella P, Tomasoni D, Faretta M, Lupi M, Montalenti F, Viale

F, Banzato F, D’Incalci M and Ubezio P: Cell cycle effects of

gemcitabine. Int J Cancer 93: 401-408, 2001. PMID: 11433406.

DOI: 10.1002/ijc.1351

Namima et al: MicroRNA Profile in Gemcitabine-treated PDAC Cells and Tumours

24 Rejiba S, Wack S, Aprahamian M and Hajri A: K-ras oncogene

silencing strategy reduces tumor growth and enhances

gemcitabine chemotherapy efficacy for pancreatic cancer

treatment. Cancer Sci 98: 1128-1136, 2007. PMID: 17489984.

DOI: 10.1111/j.1349-7006.2007.00506.x

25 Sherr CJ: G1 phase progression: cycling on cue. Cell 79: 551-555,

1994. PMID: 7954821. DOI: 10.1016/0092-8674(94)90540-1

26 Gansauge S, Gansauge F, Ramadani M, Stobbe H, Rau B,

Harada N and Beger HG: Overexpression of cyclin D1 in human

pancreatic carcinoma is associated with poor prognosis. Cancer

Res 57: 1634-1637, 1997. PMID: 9134998.

27 Zhu HQ, Ma JB, Song X, Gao HJ, Ma CQ, Chang H, Li HG,

Liu FF, Lu J and Zhou X: Metformin potentiates the anticancer

activities of gemcitabine and cisplatin against cholangiocarcinoma cells in vitro and in vivo. Oncol Rep 36: 3488-3496,

2016. PMID: 27779693. DOI: 10.3892/or.2016.5187

28 Kornmann M, Ishiwata T, Itakura J, Tangvoranuntakul P, Beger

HG and Korc M: Increased cyclin D1 in human pancreatic

cancer is associated with decreased postoperative survival.

Oncology 55: 363-369, 1998. PMID: 9663429. DOI: 10.1159/

000011879

29 Chandler NM, Canete JJ and Callery MP: Caspase-3 drives

apoptosis in pancreatic cancer cells after treatment with

gemcitabine. J Gastrointest Surg 8: 1072-1078, 2004. PMID:

15585396. DOI: 10.1016/j.gassur.2004.09.054

30 Hamed SS, Straubinger RM and Jusko WJ: Pharmaco-dynamic

modeling of cell cycle and apoptotic effects of gemcitabine on

pancreatic adenocarcinoma cells. Cancer Chemother Pharmacol

72(3): 553-563, 2013. PMID: 23835677. DOI: 10.1007/s00280013-2226-6

31 Drakaki A and Iliopoulos D: MicroRNA-gene signaling

pathways in pancreatic cancer. Biomed J 36: 200-208, 2013.

PMID: 24225187. DOI: 10.4103/2319-4170.119690

32 Meng F, Henson R, Lang M, Wehbe H, Maheshwari S, Mendell

JT, Jiang J, Schmittgen TD and Patel T: Involvement of human

micro-RNA in growth and response to chemotherapy in human

cholangiocarcinoma cell lines. Gastroenterology 130: 21132129, 2006. PMID: 16762633. DOI: 10.1053/j.gastro.

2006.02.057

33 Morishita A and Masaki T: miRNA in hepatocellular carcinoma.

Hepatology Res 45: 128-141, 2015. PMID: 25040738. DOI:

10.1111/hepr.12386

34 Yu J, Li A, Hong SM, Hruban RH and Goggins M: MicroRNA

alterations of pancreatic intraepithelial neoplasias. Clin Cancer

Res 18(4): 981-992, 2012. PMID: 22114139. DOI: 10.1158/

1078-0432.CCR-11-2347

35 Shu Z, Chen L and Ding D: miR-582-5P induces colorectal

cancer cell proliferation by targeting adenomatous polyposis

coli. World J Surg Oncol 14(1): 239, 2016. PMID: 27595705.

DOI: 10.1186/s12957-016-0984-4

Received May 28, 2020

Revised July 24, 2020

Accepted July 27, 2020

3203

...

参考文献をもっと見る

全国の大学の
卒論・修論・学位論文

一発検索!

この論文の関連論文を見る