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ACKNOWLEDGEMENT

This doctoral thesis is submitted to the Graduate School of

Advanced Science of Matter, Hiroshima University. The research was

conducted in the Laboratory of Biomolecular Technology, Department of

Molecular Biotechnology, Graduate School of Advanced Sciences of

Matter, Hiroshima University, during the academic periods from 2017 to

2020.

I would like to express the most profound appreciation to my

advisor, Prof. Junichi Kato, who has supported me before and throughout

my Ph.D. course and thesis. His knowledge and patience have always been

surprising and encouraging me. The guidance of his intelligentsia gave me

the opportunity to pursue my research in my own way. His wise support

conveyed the spirit of adventure in regard to research and teaching me.

Without his trust and persistent help, this project would not have been

possible.

My sincere thanks also go to Assistant Professor Takahisa Tajima

for his generous support and thoughtful advice. His valuable guidance,

assistance, and discussion improve my practical technical skills. I will

never forget his influential role and kindness in making this project a

success.

I would also like to thank my friends (Mr. Keisuke Hirai and Mr.

Koji Fuki), who helped me a lot in this project. I thank Professor Tatsuo

Kurihara at Kyoto University for providing S. livingstonensis Ac10.

I would like to thank all the staff of the Laboratory of Biomolecular

Technology (Associate Professor Makoto Fujie, Assistant Professor Takeru

Kawasaki, and Assistant Professor Akiko Hida) for their valuable

89

discussion and encouragement. I learned a lot about scientific research and

Japan's culture, and I am very grateful for that.

I would also like to thank my dissertation committee members,

Professor Nobukazu Tanaka, Professor Akio Kuroda, who offered

insightful comments.

Living in Japan was brilliant and astonishing for me. I would like to

appreciate all my friends, primarily those who had worked together as lab

members. Kindness, friendship, and assistance were only a small part of

what I experienced and saw from them. I owe it to myself to thank every

Japanese person for their kindness and respect.

I want to thank all my friends in Iran. We experienced moments of

support and joy together.

This thesis is dedicated to my family especially my parents, sister,

my uncle Amir and my grandmother's spirit whose constant support and

encouragement made this possible. I would like to write only one sentence

to them in the Persian language in this part.

‫ ھﺳﺗم و ﺧواھم‬،‫ داﯾﯽ اﻣﯾر ﻗدردان ﺗﻣﺎم ﻣﮭرﺑﺎﻧﯽھﺎ و ﺣﻣﺎﯾتھﺎﯾﺗﺎن ﺑوده‬،‫ ﺧواھر‬،‫ ﻣﺎدر‬،‫ﭘدر‬

.‫ دارم و ﺧواھم داﺷت‬،‫ ﻣﺎدرﺑزرگ ﻋﺎﺷﻘﺎﻧﮫ دوﺳﺗت داﺷﺗم‬.‫ﺑود‬

Finally, I would like to thank for MEXT (Ministry of Education,

Culture, Sports, Science and Technology) Scholarship supported by the

Japanese government for the valuable supporting and great opportunity to

study at Hiroshima University, Japan.

90

බ⾲ㄽᩥ

㸦Articles㸧

(1) Efficient

production

of

1,3-propanediol

by

psychrophile-based

simple

biocatalysts in Shewanella livingstonensis Ac10 and Shewanella frigidimarina

DSM 12253.

Mohammad Mojarrad, Keisuke Hirai, Koji Fuki, Takahisa Tajima, Akiko Hida,

Junichi Kato

Journal of Biotechnology, 323 (1), 293-301 (2020).

(2) Psychrophile-based simple biocatalysts for effective coproduction of

3-hydroxypropionic acid and 1,3-propanediol.

Mohammad Mojarrad, Takahisa Tajima, Akiko Hida, Junichi Kato

Bioscience, Biotechnology, and Biochemistry, DOI: 10.1093/bbb/zbaa081.

91

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