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FIGURE LEGENDS
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Figure 1. Macroscopic and histological features of control and IBD colons.
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Mucous membranes of colons (A) in control (upper) and IBD (lower) rats. IBD and
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control rats were injected with TNBS or saline at 80 mm from anus. Hematoxylin-eosin
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stain (B–D) of control (B) and of low (LMIG; C) and high (HMIG; D) migration regions
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of IBD colons. Asterisk and arrowhead in (D) indicate deformed intestinal crypts and area
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without epithelium, respectively. Bars = 10 mm (A) and 100 (B, C) and 50 (D) μm.
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Figure 2. Ultrastructural features of crypt goblet cells in control and IBD colons.
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Transmission electron microscopy (TEM; A–C) and osmium-maceration scanning
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electron microscopy (SEM; D, E) findings of control (A, D), LMIG (B, E) and HMIG
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(C). Abbreviations: sg, secretory granules; v, vacuolar structure. *Lumen (A–C). Red
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highlights, mucous fluid covering epithelial surface; green highlights, secretory granules
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in (D, E). Bars = 2 μm.
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Figure 3. Ultrastructural features of perinuclear region of absorption epithelial cells of
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control and IBD colons.
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Findings of TEM (A–C) and osmium-maceration SEM (D, E) of control (A, D), LMIG
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(B, E) and HMIG (C). Arrowheads (A–C) indicate Golgi apparatus. Red and blue
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highlights (D, E) indicate Golgi apparatus and nuclei, respectively. Bars = 1 μm.
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Figure 4. Ultrastructural features of apical region of absorption epithelial cells in control
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and IBD colons.
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Findings of TEM (A–C) and osmium-maceration SEM (D–F) of control (A, D), LMIG
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(B, E) and HMIG (C, F). Arrowheads (A–C) indicate small vesicular structures. Red and
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green highlights (D–F) indicate membrane network structures and mitochondria,
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respectively. Abbreviations: mv, microvilli; *Space without cytosol (D–F). Bars = 1 μm.
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Figure S1. Distribution of immune cells and polysaccharide in control and model colons.
Anti-Iba1 (Code 019-19741; Wako Pure Chemical Industries Ltd., Osaka, Japan) immunostain
(A–C) and toluidine blue stain (D–F) to detect macrophages and mast cells, respectively, in
control (A, D), LMIG (B, E) and HMIG (C, F). Arrowheads (A–C) indicate Iba1-positive
macrophages; arrowheads (D–F) indicate metachromatic mast cells. Periodic acid-Schiff
reaction (G–I) and Alcian blue (pH 2.5) reaction (J–L) to detect neutral and acidic
polysaccharide, respectively. Inserts in (I, L) show high magnification of intestinal crypts. Bars
= 100 (A, B, G, H, J, K), 50 (C, I, L), 20 (D–F) μm.
Figure S1
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