Ali AK, Nandagopal N, Lee SH. 2015. IL-15-Pi3K-AKT-mTOR: a critical pathway in the life journey of natural
killer cells. Frontiers in Immunology 6:355. DOI: https://doi.org/10.3389/fimmu.2015.00355, PMID:
26257729
Bai L, Vienne M, Tang L, Kerdiles Y, Etiennot M, Escalière B, Galluso J, Wei H, Sun R, Vivier E, Peng H, Tian Z.
2021. Liver type 1 innate Lymphoid cells develop locally via an interferon-Γ-dependent loop. Science
371:eaba4177. DOI: https://doi.org/10.1126/science.aba4177, PMID: 33766856
Bhat R, Watzl C, Zimmer J. 2007. Serial killing of tumor cells by human natural killer cells--enhancement by
therapeutic antibodies. PLOS ONE 2:e326. DOI: https://doi.org/10.1371/journal.pone.0000326
Cepero-Donates Y, Rakotoarivelo V, Mayhue M, Ma A, Chen YG, Ramanathan S. 2016. Homeostasis of IL-15
dependent lymphocyte Subsets in the liver. Cytokine 82:95–101. DOI: https://doi.org/10.1016/j.cyto.2015.12.
012
Chen S, Zhou Y, Chen Y, Gu J. 2018. Fastp: an ultra-fast all-in-one FASTQ Preprocessor. Bioinformatics 34:i884–
i890. DOI: https://doi.org/10.1093/bioinformatics/bty560
Chen Y, Wang X, Hao X, Li B, Tao W, Zhu S, Qu K, Wei H, Sun R, Peng H, Tian Z. 2022. Ly49E separates liver Ilc1S
into embryo-derived and postnatal Subsets with different functions. The Journal of Experimental Medicine
219:e20211805. DOI: https://doi.org/10.1084/jem.20211805, PMID: 35348580
Constantinides MG, McDonald BD, Verhoef PA, Bendelac A. 2014. A committed precursor to innate Lymphoid
cells. Nature 508:397–401. DOI: https://doi.org/10.1038/nature13047, PMID: 24509713
Constantinides MG, Gudjonson H, McDonald BD, Ishizuka IE, Verhoef PA, Dinner AR, Bendelac A. 2015. PLZF
expression maps the early stages of Ilc1 lineage development. PNAS 112:5123–5128. DOI: https://doi.org/10.
1073/pnas.1423244112, PMID: 25838284
Cui G, Hara T, Simmons S, Wagatsuma K, Abe A, Miyachi H, Kitano S, Ishii M, Tani-ichi S, Ikuta K. 2014.
Characterization of the IL-15 niche in primary and secondary Lymphoid organs in vivo. PNAS 111:1915–1920.
DOI: https://doi.org/10.1073/pnas.1318281111, PMID: 24449915
Dadi S, Chhangawala S, Whitlock BM, Franklin RA, Luo CT, Oh SA, Toure A, Pritykin Y, Huse M, Leslie CS, Li MO.
2016. Cancer Immunosurveillance by tissue-resident innate Lymphoid cells and innate-like T cells. Cell
164:365–377. DOI: https://doi.org/10.1016/j.cell.2016.01.002, PMID: 26806130
Dahlgren MW, Jones SW, Cautivo KM, Dubinin A, Ortiz-Carpena JF, Farhat S, Yu KS, Lee K, Wang C,
Molofsky AV, Tward AD, Krummel MF, Peng T, Molofsky AB. 2019. Adventitial Stromal cells define group 2
Asahi et al. eLife 2023;12:e84209. DOI: https://doi.org/10.7554/eLife.84209
19 of 22
Immunology and Inflammation
Research article
innate Lymphoid cell tissue niches. Immunity 50:707–722.. DOI: https://doi.org/10.1016/j.immuni.2019.02.002,
PMID: 30824323
Daussy C, Faure F, Mayol K, Viel S, Gasteiger G, Charrier E, Bienvenu J, Henry T, Debien E, Hasan UA, Marvel J,
Yoh K, Takahashi S, Prinz I, de Bernard S, Buffat L, Walzer T. 2014. T-bet and Eomes instruct the development of
two distinct natural killer cell lineages in the liver and in the bone marrow. The Journal of Experimental
Medicine 211:563–577. DOI: https://doi.org/10.1084/jem.20131560, PMID: 24516120
Di Censo C, Marotel M, Mattiola I, Müller L, Scarno G, Pietropaolo G, Peruzzi G, Laffranchi M, Mazej J,
Hasim MS, Asif S, Russo E, Tomaipitinca L, Stabile H, Lee SH, Vian L, Gadina M, Gismondi A, Shih HY, Mikami Y,
et al. 2021. Granzyme A and Cd160 expression Delineates Ilc1 with graded functions in the mouse liver.
European Journal of Immunology 51:2568–2575. DOI: https://doi.org/10.1002/eji.202149209, PMID: 34347289
Fehniger TA, Cai SF, Cao X, Bredemeyer AJ, Presti RM, French AR, Ley TJ. 2007. Acquisition of murine NK cell
cytotoxicity requires the translation of a pre-existing pool of Granzyme B and Perforin mRNAs. Immunity
26:798–811. DOI: https://doi.org/10.1016/j.immuni.2007.04.010, PMID: 17540585
Friedrich C, Taggenbrock RLRE, Doucet-Ladevèze R, Golda G, Moenius R, Arampatzi P, Kragten NAM,
Kreymborg K, Gomez de Agüero M, Kastenmüller W, Saliba A-E, Grün D, van Gisbergen KPJM, Gasteiger G.
2021. Effector differentiation downstream of lineage commitment in Ilc1S is driven by Hobit across tissues.
Nature Immunology 22:1256–1267. DOI: https://doi.org/10.1038/s41590-021-01013-0, PMID: 34462601
Gasteiger G, Fan X, Dikiy S, Lee SY, Rudensky AY. 2015. Tissue Residency of innate Lymphoid cells in Lymphoid
and Nonlymphoid organs. Science 350:981–985. DOI: https://doi.org/10.1126/science.aac9593, PMID:
26472762
Gwalani LA, Orange JS. 2018. Single Degranulations in NK cells can mediate target cell killing. Journal of
Immunology 200:3231–3243. DOI: https://doi.org/10.4049/jimmunol.1701500, PMID: 29592963
Ikuta K, Hara T, Abe S, Asahi T, Takami D, Cui G. 2021. The roles of IL-7 and IL-15 in niches for lymphocyte
Progenitors and immune cells in Lymphoid organs. Current Topics in Microbiology and Immunology 434:83–
101. DOI: https://doi.org/10.1007/978-3-030-86016-5_4, PMID: 34850283
Jacquelot N, Seillet C, Vivier E, Belz GT. 2022. Innate Lymphoid cells and cancer. Nature Immunology 23:371–
379. DOI: https://doi.org/10.1038/s41590-022-01127-z, PMID: 35228695
Kansler ER, Dadi S, Krishna C, Nixon BG, Stamatiades EG, Liu M, Kuo F, Zhang J, Zhang X, Capistrano K,
Blum KA, Weiss K, Kedl RM, Cui G, Ikuta K, Chan TA, Leslie CS, Hakimi AA, Li MO. 2022. Cytotoxic innate
Lymphoid cells sense cancer cell-expressed Interleukin-15 to suppress human and murine malignancies. Nature
Immunology 23:904–915. DOI: https://doi.org/10.1038/s41590-022-01213-2
Kataoka T, Takaku K, Magae J, Shinohara N, Takayama H, Kondo S, Nagai K. 1994. Acidification is essential for
maintaining the structure and function of Lytic granules of CTL. effect of Concanamycin A, an inhibitor of
vacuolar type H(+)-Atpase, on CTL-mediated cytotoxicity. Journal of Immunology 153:3938–3947 PMID:
7930604.
Khan JA, Mendelson A, Kunisaki Y, Birbrair A, Kou Y, Arnal-Estapé A, Pinho S, Ciero P, Nakahara F, Ma’ayan A,
Bergman A, Merad M, Frenette PS. 2016. Fetal liver hematopoietic stem cell niches associate with portal
vessels. Science 351:176–180. DOI: https://doi.org/10.1126/science.aad0084, PMID: 26634440
Klose CSN, Flach M, Möhle L, Rogell L, Hoyler T, Ebert K, Fabiunke C, Pfeifer D, Sexl V, Fonseca-Pereira D,
Domingues RG, Veiga-Fernandes H, Arnold SJ, Busslinger M, Dunay IR, Tanriver Y, Diefenbach A. 2014.
Differentiation of type 1 Ilcs from a common progenitor to all helper-like innate Lymphoid cell lineages. Cell
157:340–356. DOI: https://doi.org/10.1016/j.cell.2014.03.030, PMID: 24725403
Kotas ME, Locksley RM. 2018. Why innate Lymphoid cells Immunity 48:1081–1090. DOI: https://doi.org/10.
1016/j.immuni.2018.06.002, PMID: 29924974
Krabbendam L, Bernink JH, Spits H. 2021. Innate Lymphoid cells: from helper to killer. Current Opinion in
Immunology 68:28–33. DOI: https://doi.org/10.1016/j.coi.2020.08.007, PMID: 32971468
Lewis K, Yoshimoto M, Takebe T. 2021. Fetal liver Hematopoiesis: from development to delivery. Stem Cell
Research & Therapy 12:139. DOI: https://doi.org/10.1186/s13287-021-02189-w, PMID: 33597015
Liang B, Hara T, Wagatsuma K, Zhang J, Maki K, Miyachi H, Kitano S, Yabe-Nishimura C, Tani-Ichi S, Ikuta K.
2012. Role of hepatocyte-derived IL-7 in maintenance of Intrahepatic NKT cells and T cells and development of
B cells in fetal liver. Journal of Immunology 189:4444–4450. DOI: https://doi.org/10.4049/jimmunol.1201181,
PMID: 23018454
Lim HY, Lim SY, Tan CK, Thiam CH, Goh CC, Carbajo D, Chew SHS, See P, Chakarov S, Wang XN, Lim LH,
Johnson LA, Lum J, Fong CY, Bongso A, Biswas A, Goh C, Evrard M, Yeo KP, Basu R, et al. 2018. Hyaluronan
receptor LYVE-1-expressing Macrophages maintain arterial tone through Hyaluronan-mediated regulation of
smooth muscle cell collagen. Immunity 49:1191. DOI: https://doi.org/10.1016/j.immuni.2018.12.009, PMID:
30566884
Liou YH, Wang SW, Chang CL, Huang PL, Hou MS, Lai YG, Lee GA, Jiang ST, Tsai CY, Liao NS. 2014. Adipocyte
IL-15 regulates local and systemic NK cell development. Journal of Immunology 193:1747–1758. DOI: https://
doi.org/10.4049/jimmunol.1400868, PMID: 25009203
Lodolce JP, Boone DL, Chai S, Swain RE, Dassopoulos T, Trettin S, Ma A. 1998. IL-15 receptor maintains
Lymphoid homeostasis by supporting lymphocyte homing and proliferation. Immunity 9:669–676. DOI: https://
doi.org/10.1016/s1074-7613(00)80664-0, PMID: 9846488
Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR,
Lein ES, Zeng H. 2010. A robust and high-throughput CRE reporting and characterization system for the whole
mouse brain. Nature Neuroscience 13:133–140. DOI: https://doi.org/10.1038/nn.2467, PMID: 20023653
Asahi et al. eLife 2023;12:e84209. DOI: https://doi.org/10.7554/eLife.84209
20 of 22
Immunology and Inflammation
Research article
Marçais A, Cherfils-Vicini J, Viant C, Degouve S, Viel S, Fenis A, Rabilloud J, Mayol K, Tavares A, Bienvenu J,
Gangloff YG, Gilson E, Vivier E, Walzer T. 2014. The metabolic Checkpoint kinase mTOR is essential for IL-15
signaling during the development and activation of NK cells. Nature Immunology 15:749–757. DOI: https://doi.
org/10.1038/ni.2936, PMID: 24973821
McFarland AP, Colonna M. 2020. Sense and Immuno-sensibility: innate Lymphoid cell niches and circuits.
Current Opinion in Immunology 62:9–14. DOI: https://doi.org/10.1016/j.coi.2019.11.003, PMID: 31825814
McFarland AP, Yalin A, Wang SY, Cortez VS, Landsberger T, Sudan R, Peng V, Miller HL, Ricci B, David E,
Faccio R, Amit I, Colonna M. 2021. Multi-tissue single-cell analysis Deconstructs the complex programs of
mouse natural killer and type 1 innate Lymphoid cells in tissues and circulation. Immunity 54:1320–1337. DOI:
https://doi.org/10.1016/j.immuni.2021.03.024, PMID: 33945787
Mortier E, Woo T, Advincula R, Gozalo S, Ma A. 2008. IL-15Ralpha Chaperones IL-15 to stable Dendritic cell
membrane complexes that activate NK cells via Trans presentation. The Journal of Experimental Medicine
205:1213–1225. DOI: https://doi.org/10.1084/jem.20071913, PMID: 18458113
Mortier E, Advincula R, Kim L, Chmura S, Barrera J, Reizis B, Malynn BA, Ma A. 2009. Macrophage- and
Dendritic-cell-derived Interleukin-15 receptor alpha supports homeostasis of distinct Cd8+ T cell Subsets.
Immunity 31:811–822. DOI: https://doi.org/10.1016/j.immuni.2009.09.017
Murphy JM, Ngai L, Mortha A, Crome SQ. 2022. Tissue-dependent adaptations and functions of innate
Lymphoid cells. Frontiers in Immunology 13:836999. DOI: https://doi.org/10.3389/fimmu.2022.836999, PMID:
35359972
Nabekura T, Lanier LL. 2016. Tracking the fate of antigen-specific versus cytokine-activated natural killer cells
after Cytomegalovirus infection. The Journal of Experimental Medicine 213:2745–2758. DOI: https://doi.org/
10.1084/jem.20160726, PMID: 27810928
Nabekura T, Riggan L, Hildreth AD, O’Sullivan TE, Shibuya A. 2020. Type 1 innate Lymphoid cells protect mice
from acute liver injury via interferon-Γ secretion for Upregulating bcl-xL expression in hepatocytes. Immunity
52:96–108.. DOI: https://doi.org/10.1016/j.immuni.2019.11.004, PMID: 31810881
Nandagopal N, Ali AK, Komal AK, Lee SH. 2014. The critical role of IL-15-Pi3K-mTOR pathway in natural killer
cell Effector functions. Frontiers in Immunology 5:187. DOI: https://doi.org/10.3389/fimmu.2014.00187, PMID:
24795729
Nixon BG, Chou C, Krishna C, Dadi S, Michel AO, Cornish AE, Kansler ER, Do MH, Wang X, Capistrano KJ,
Rudensky AY, Leslie CS, Li MO. 2022. Cytotoxic Granzyme C-expressing Ilc1S contribute to antitumor immunity
and neonatal Autoimmunity. Science Immunology 7:eabi8642. DOI: https://doi.org/10.1126/sciimmunol.
abi8642, PMID: 35394814
Peng H, Jiang X, Chen Y, Sojka DK, Wei H, Gao X, Sun R, Yokoyama WM, Tian Z. 2013. Liver-resident NK cells
confer adaptive immunity in skin-contact inflammation. The Journal of Clinical Investigation 123:1444–1456.
DOI: https://doi.org/10.1172/JCI66381, PMID: 23524967
Pham THM, Baluk P, Xu Y, Grigorova I, Bankovich AJ, Pappu R, Coughlin SR, McDonald DM, Schwab SR,
Cyster JG. 2010. Lymphatic endothelial cell Sphingosine kinase activity is required for lymphocyte Egress and
Lymphatic Patterning. The Journal of Experimental Medicine 207:17–27. DOI: https://doi.org/10.1084/jem.
20091619, PMID: 20026661
Postic C, Shiota M, Niswender KD, Jetton TL, Chen Y, Moates JM, Shelton KD, Lindner J, Cherrington AD,
Magnuson MA. 1999. Dual roles for Glucokinase in glucose homeostasis as determined by liver and Pancreatic
Β cell-specific gene knock-outs using CRE Recombinase. Journal of Biological Chemistry 274:305–315. DOI:
https://doi.org/10.1074/jbc.274.1.305
Prager I, Liesche C, van Ooijen H, Urlaub D, Verron Q, Sandström N, Fasbender F, Claus M, Eils R, Beaudouin J,
Önfelt B, Watzl C. 2019. NK cells switch from Granzyme B to death receptor-mediated cytotoxicity during serial
killing. The Journal of Experimental Medicine 216:2113–2127. DOI: https://doi.org/10.1084/jem.20181454,
PMID: 31270246
Robinette ML, Bando JK, Song W, Ulland TK, Gilfillan S, Colonna M. 2017. IL-15 sustains IL-7R-independent Ilc2
and Ilc3 development. Nature Communications 8:14601. DOI: https://doi.org/10.1038/ncomms14601, PMID:
28361874
Sojka DK, Plougastel-Douglas B, Yang L, Pak-Wittel MA, Artyomov MN, Ivanova Y, Zhong C, Chase JM,
Rothman PB, Yu J, Riley JK, Zhu J, Tian Z, Yokoyama WM. 2014. Tissue-resident natural killer (NK) cells are cell
lineages distinct from Thymic and conventional splenic NK cells. eLife 3:e01659. DOI: https://doi.org/10.7554/
eLife.01659, PMID: 24714492
Song J, Song H, Wei H, Sun R, Tian Z, Peng H. 2021. Requirement of RORα for maintenance and antitumor
immunity of Liver‐Resident natural killer cells/Ilc1S. Hepatology 75:1181–1193. DOI: https://doi.org/10.1002/
hep.32147, PMID: 34510508
Sparano C, Solís-Sayago D, Vijaykumar A, Rickenbach C, Vermeer M, Ingelfinger F, Litscher G, Fonseca A,
Mussak C, Mayoux M, Friedrich C, Nombela-Arrieta C, Gasteiger G, Becher B, Tugues S. 2022. Embryonic and
neonatal waves generate distinct populations of hepatic Ilc1S. Science Immunology 7:eabo6641. DOI: https://
doi.org/10.1126/sciimmunol.abo6641, PMID: 36054340
Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, Costantini F. 2001. Cre reporter strains
produced by targeted insertion of EYFP and ECFP into the Rosa26 locus. BMC Developmental Biology 1:4.
DOI: https://doi.org/10.1186/1471-213x-1-4, PMID: 11299042
Stark R, Wesselink TH, Behr FM, Kragten NAM, Arens R, Koch-Nolte F. 2018. TRM maintenance is regulated by
tissue damage via P2Rx7. Science Immunology 3:eaau1022. DOI: https://doi.org/10.1126/sciimmunol.aau1022
Asahi et al. eLife 2023;12:e84209. DOI: https://doi.org/10.7554/eLife.84209
21 of 22
Immunology and Inflammation
Research article
Stokic-Trtica V, Diefenbach A, Klose CSN. 2020. NK cell development in times of innate Lymphoid cell diversity.
Frontiers in Immunology 11:813. DOI: https://doi.org/10.3389/fimmu.2020.00813, PMID: 32733432
Vivier E, Artis D, Colonna M, Diefenbach A, Di Santo JP, Eberl G, Koyasu S, Locksley RM, McKenzie ANJ,
Mebius RE, Powrie F, Spits H. 2018. Innate Lymphoid cells: 10 years on. Cell 174:1054–1066. DOI: https://doi.
org/10.1016/j.cell.2018.07.017, PMID: 30142344
Wang X, Peng H, Cong J, Wang X, Lian Z, Wei H, Sun R, Tian Z. 2018. Memory formation and long-term
maintenance of IL-7Rα+ Ilc1S via a lymph node-liver axis. Nature Communications 9:4854. DOI: https://doi.org/
10.1038/s41467-018-07405-5, PMID: 30451860
Weizman OE, Adams NM, Schuster IS, Krishna C, Pritykin Y, Lau C, Degli-Esposti MA, Leslie CS, Sun JC,
O’Sullivan TE. 2017. Ilc1 confer early host protection at initial sites of viral infection. Cell 171:795–808.. DOI:
https://doi.org/10.1016/j.cell.2017.09.052, PMID: 29056343
Weizman OE, Song E, Adams NM, Hildreth AD, Riggan L, Krishna C, Aguilar OA, Leslie CS, Carlyle JR, Sun JC,
O’Sullivan TE. 2019. Mouse Cytomegalovirus-experienced Ilc1S acquire a memory response dependent on the
viral glycoprotein M12. Nature Immunology 20:1004–1011. DOI: https://doi.org/10.1038/s41590-019-0430-1,
PMID: 31263280
Yomogida K, Bigley TM, Trsan T, Gilfillan S, Cella M, Yokoyama WM, Egawa T, Colonna M. 2021. Hobit confers
tissue-dependent programs to type 1 innate Lymphoid cells. PNAS 118:e2117965118. DOI: https://doi.org/10.
1073/pnas.2117965118, PMID: 34880136
Yu Y, Tsang JCH, Wang C, Clare S, Wang J, Chen X, Brandt C, Kane L, Campos LS, Lu L, Belz GT, McKenzie ANJ,
Teichmann SA, Dougan G, Liu P. 2016. Single-cell RNA-Seq identifies a PD-1Hi ILC progenitor and defines its
development pathway. Nature 539:102–106. DOI: https://doi.org/10.1038/nature20105, PMID: 27749818
Zhou Y, Zhou B, Pache L, Chang M, Khodabakhshi AH, Tanaseichuk O, Benner C, Chanda SK. 2019. Metascape
provides a biologist-oriented resource for the analysis of systems-level Datasets. Nature Communications
10:1523. DOI: https://doi.org/10.1038/s41467-019-09234-6, PMID: 30944313
Asahi et al. eLife 2023;12:e84209. DOI: https://doi.org/10.7554/eLife.84209
22 of 22
...