論文の公開元へHost genetics highlights IFN-γ-dependent Toxoplasma genes encoding secreted and non-secreted virulence factors in in vivo CRISPR screens
概要
Toxoplasma is an important protozoan pathogen that can cause
life-threatening toxoplasmosis in humans and animals.1,2 To
date, most of the Toxoplasma virulence genes have been identified through classical forward and reverse genetics methods.
The forward genetics identified several virulence genes such
as ROP5, ROP16, and ROP18,3–6 which are family members of
rhoptry bulb proteins (ROPs) secreted into host cells during
Toxoplasma infection.7,8 Those ROPs suppress host immunity
and thus contribute to the parasite in vivo fitness.9–14 Although
forward genetics is a powerful strategy, the method relies on
polymorphisms in the virulence genes. The classical reverse genetics using individual gene-knockout Toxoplasma is solid; however, the gene of interest is intentionally selected. It also remains
unclear whether the gene is involved in virulence. ...
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