論文の公開元へNovel and efficient method for culturing patient-derived gastric cancer stem cells
概要
Experimental techniques for patient-derived cancer stem-cell organoids/spheroids
can be powerful diagnostic tools for personalized chemotherapy. However, establishing their cultures from gastric cancer remains challenging due to low culture efficiency
and cumbersome methods. To propagate gastric cancer cells as highly proliferative
stem-cell spheroids in vitro, we initially used a similar method to that for colorectal
cancer stem cells, which, unfortunately, resulted in a low success rate (25%, 18 of
71 cases). We scrutinized the protocol and found that the unsuccessful cases were
largely caused by the paucity of cancer stem cells in the sampled tissues as well as
insufficient culture media. To overcome these obstacles, we extensively revised our
sample collection protocol and culture conditions. We then investigated the following
second cohort and, consequently, achieved a significantly higher success rate (88%,
29 of 33 cases). ...
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