論文の公開元へmiR-125b accumulated in bone matrix suppresses osteolytic bone metastasis
概要
miR-125b accumulated in bone matrix suppresses osteolytic bone
metastasis
（骨基質由来 miR-125b は溶骨性骨転移を抑制する）
Ph.D. Applicant Nushrat Sarmin
Department of Calcified Tissue Biology
Biomedical Science Major
Graduate School of Biomedical and Health Sciences
Hiroshima University
Supervisor: Yuji Yoshiko, Ph.D.
2020
Contents
1.
Abstract ..................................................................................................... 1
2.
Introduction .............................................................................................. 2
3.
Materials and Methods ............................................................................ 5
3.1.
Animals
3.2.
Chemicals
3.3.
Cell cultures
3.4.
PCR
3.5.
MV isolation
3.6.
Protein, alkaline phosphatase (ALPL) assay
3.7.
MTT and EdU assay
3.8.
Wound healing assay
3.9.
Trans-well invasion assay
3.10.
Trans-well migration assay
3.11.
Transfection of cells with miR-125b mimic
3.12.
Py8119 cells expressing luciferase
3.13.
Mouse model of bone metastasis
3.14.
Micro-computer tomography ( CT)
3.15.
In vivo imaging
3.16.
Histology
3.17.
miRNA database for candidate target genes
3.18.
Statistical Analysis
4.
Results ....................................................................................................... 12
4.1.
MVs and miR-125b suppresses proliferation, migration and/or
invasion of cancer cells
4.2.
MiR-125b diminish bone loses caused by cancer cells progression
4.3.
Candidate Target genes of miR-125b in Py8119 cells
5.
Discussion .................................................................................................. 15
6.
Figure legends ........................................................................................... 19
7.
Figures and tables ..................................................................................... 21
8.
References .................................................................................................. 29
