暑熱ストレス下における反芻動物の乳腺自然免疫機能に関する研究

概要




༤ኈㄽᩥ

ᬬ⇕ࢫࢺࣞࢫୗ࡟࠾ࡅࡿ
཯ⱄື≀ࡢங⭢⮬↛ච␿ᶵ⬟࡟㛵ࡍࡿ◊✲




㸦せ⣙㸧



௧࿴3ᖺ3᭶
ᗈᓥ኱Ꮫ኱Ꮫ㝔⏕≀ᅪ⛉Ꮫ◊✲⛉
⏕≀㈨※⛉Ꮫᑓᨷ
㕥ᮌ┤ᶞ

せ ⣙

  ㄽᩥ㢟┠   ᬬ⇕ࢫࢺࣞࢫୗ࡟࠾ࡅࡿ཯ⱄື≀ࡢங⭢⮬↛ච␿ᶵ⬟࡟㛵ࡍࡿ◊✲
 

࢘ࢩࡢஙᡣ⅖ࡣኟ࡟ከ࠸࡜ゝࢃࢀ࡚࠸ࡿࡀ, ࡇࡢཎᅉࡢ୍ࡘ࡟ᬬ⇕ࢫࢺࣞࢫࡀங⭢ࡢ⮬↛
ච␿ᶵ⬟ࢆపୗࡉࡏ, ឤᰁࡋࡸࡍࡃ࡞ࡗ࡚࠸ࡿྍ⬟ᛶࡀ࠶ࡿࠋࡑࡇ࡛, ᮏ◊✲ࡣ, ᬬ⇕ࢫࢺࣞ
ࢫࡀ཯ⱄື≀ங⭢ࡢ⮬↛ච␿ᶵ⬟࡟ཬࡰࡍᙳ㡪ࢆㄪ࡭ࡿࡇ࡜ࢆ┠ⓗ࡜ࡋࡓࠋࡲࡎ, Ꮨ⠇ࡀ࢘
ࢩࡢங⭢⮬↛ච␿ᶵ⬟࡟ཬࡰࡍᙳ㡪ࢆㄪ࡭ࡿࡓࡵ࡟, bulk milk somatic cell count㸦BMSCC㸧ࡢ
᭶ኚ໬ࢆㄪ࡭ࡿ࡜࡜ࡶ࡟, ஙỒ୰ᢠ⳦ᅉᏊ⃰ᗘ࠾ࡼࡧᬬ⇕ࢫࢺࣞࢫᣦᶆࢆᏘ⠇㛫࡛ẚ㍑ࡋࡓࠋ
ḟ࡟, ࣖࢠࢆ⏝࠸࡚ᬬ⇕ࢫࢺࣞࢫ࡟ࡼࡾ㉳ࡇࡿ࡜ࡉࢀࡿ⏥≧⭢ᶵ⬟పୗ࠾ࡼࡧ㧗 cortisol ≧
ែࢆ෌⌧ࡋ, ஙᡣෆ࡟ lipopolysaccharide㸦LPS㸧ࢆὀධࡋࡓᚋࡢ⮬↛ච␿ᶵ⬟ࡢኚ໬ࢆㄪ࡭ࡓࠋ
1. ࢘ࢩஙỒ୰ᢠ⳦ᅉᏊ⃰ᗘࡢᏘ⠇ኚ໬
 ᗈᓥ┴ࡢẼ , ┦ᑐ‵ᗘ࠿ࡽ temperature humidity index㸦THI㸧ࢆィ⟬ࡋ, BMSCC ࡶẖ᭶ㄪ
࡭ࡓࠋࡲࡓ, ෤㸦1 ࠾ࡼࡧ 2 ᭶㸧, ᫓㸦4 ࠾ࡼࡧ 5 ᭶㸧, ኟ㸦7 ࠾ࡼࡧ 8 ᭶㸧, ⛅㸦10 ࠾ࡼࡧ
11 ᭶㸧࡟, ศፔᚋ 5-8 ᪥ࡢ࣍ࣝࢫࢱ࢖ࣥ✀ங∵࠿ࡽஙỒ࠾ࡼࡧ⾑ᾮࢆ᥇ྲྀࡋࡓࠋங୰ somatic
cell count㸦SCC㸧ࡀ 300,000 cells/ml ௨ୗࡢ೺ᗣ࡞ஙỒࢆ㑅ᢥࡋ, ங୰ᢠ⳦ᅉᏊ࡜ࡋ࡚ lactoferrin,
lingual antimicrobial peptide㸦LAP㸧, S100A7 ࠾ࡼࡧ IgA ⃰ᗘ, ᬬ⇕ࢫࢺࣞࢫᣦᶆ࡜ࡋ࡚┤⭠ ,
⾑୰ diacron - reactive oxygen metabolites㸦d-ROMs㸧, cortisol, thyroxine㸦T4㸧⃰ᗘࢆ ᐃࡋࡓࠋ
THI ࡣኟ࡟ୖ᪼ࡋ, 8 ᭶࡟᭱㧗್ࢆ♧ࡋࡓࠋBMSCC ࡣ 8 ࠾ࡼࡧ 9 ᭶ࡀ 4 ࠾ࡼࡧ 5 ᭶࡟ẚ࡭࡚
᭷ព࡟㧗࠿ࡗࡓࠋSCC ࡣᏘ⠇࡟ࡼࡗ࡚㐪࠸ࡀㄆࡵࡽࢀ࡞࠿ࡗࡓࡀ, SCC ࢆ 300,000 cells/ml
௨ୗ, 300,000 㹼 1,000,000 cells/ml, 1,000,000 cells/ml ௨ୖ࡟ศ㢮ࡋ, ࡑࢀࡒࢀࡢ๭ྜࢆィ⟬
ࡍࡿ࡜, 300,000 cells/ml ௨ୗ࡜࡞ࡿஙỒࡢ๭ྜࡣ, ኟ࡟࠾࠸࡚⛅ࡢࡑࢀࡼࡾࡶ᭷ព࡟ప࠿ࡗ
ࡓࠋLactoferrin ࠾ࡼࡧ LAP ⃰ᗘࡣኟ࡟࠾࠸࡚ࡑࢀࡒࢀ෤࠾ࡼࡧ᫓ࡢࡑࢀࡽ࡜ẚ࡭࡚᭷ព࡟ప
࠿ࡗࡓࠋᬬ⇕ࢫࢺࣞࢫᣦᶆ࡛࠶ࡿ┤⭠ ᗘࡣኟࡀࡑࡢ௚ࡢᏘ⠇࡟ẚ࡭᭷ព࡟㧗࠿ࡗࡓࡀ, ⾑
୰ d-ROMs, cortisol ࠾ࡼࡧ T4 ⃰ᗘࡣᏘ⠇࡟ࡼࡿ᭷ព࡞ኚ໬ࡣ࡞࠿ࡗࡓࠋ
 ௨ୖࡢ⤖ᯝ࠿ࡽ, ౪ヨ∵ࡣኟ࡟࠶ࡿ⛬ᗘࡢᬬ⇕ࢫࢺࣞࢫࢆཷࡅ࡚࠸ࡿ࡜⪃࠼ࡽࢀࡓࠋࡲࡓ,
ኟ࡟୍㒊ࡢᢠ⳦ᅉᏊ⃰ᗘࡀపୗࡍࡿࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡗࡓࠋ
2. Propylthiouracil ࠾ࡼࡧ dexamethasone ฎ⌮ࡋࡓࣖࢠ࡟࠾ࡅࡿ LPS ᢞ୚ᚋࡢங⭢⮬↛ච␿
ᶵ⬟ࡢኚ໬
࢘ࢩࡢ⾑୰ T4 ࠾ࡼࡧ corotisol ⃰ᗘࡀᏘ⠇㛫࡛ኚ໬ࡏࡎ, ᬬ⇕ࢫࢺࣞࢫୗ࡛ኚ໬ࡍࡿෆศἪ
ᶵ⬟࡜ங⭢⮬↛ච␿ᶵ⬟ࡢ㛵ಀࡣ᫂ࡽ࠿࡟ࡉࢀ࡞࠿ࡗࡓࠋࡑࡇ࡛, ᐇ㦂ⓗ࡟⏥≧⭢ᶵ⬟ࢆప
ୗ࠾ࡼࡧ cortisol ⃰ᗘࢆୖ᪼ࡉࡏࡓ᫬ࡢங⭢⮬↛ච␿ᶵ⬟ࢆㄪ࡭ࡿ࡜࡜࡜ࡶ࡟, ࡑࡢ᫬࡟ங
ᡣෆ࡬ LPS ࢆᢞ୚ࡋࡓ᫬ࡢ⮬↛ච␿ᶵ⬟ࡢኚ໬ࢆㄪ࡭ࡓࠋࢺ࢝ࣛ✀࣓ࢫࣖࢠ࡟, ⏥≧⭢ᶵ⬟
పୗࢆ෌⌧ࡍࡿ┠ⓗ࡛ 3 㐌㛫ẖ᪥ propylthiouracil ࢆᢞ୚ࡋࡓ PTU ༊㸦⏥≧⭢ᶵ⬟పୗฎ⌮, n
= 6㸧, 㧗 cortisol ≧ែࢆ෌⌧ࡍࡿ┠ⓗ࡛ 5 ᪥㛫ẖ᪥ dexamathasone ࢆᢞ୚ࡋࡓ DEX ༊㸦㧗 cortisol
ฎ⌮, n = 6㸧࠾ࡼࡧ࠸ࡎࢀࡶฎ⌮ࡋ࡞࠸↓ฎ⌮༊㸦n = 6㸧ࢆ⏝ពࡋ, ࡑࡢᕥஙᡣ࡟ LPS 50 Pg/5
ml ⁛⳦⏕⌮㣗ሷỈࢆὀධࡋ, ྑഃஙᡣ࡟⁛⳦⏕⌮㣗ሷỈࡢࡳࢆὀධࡋࡓࠋLPS ὀධᚋ 0, 2, 4,
6, 8, 12 h ᚋ࠾ࡼࡧ 7 ᪥ᚋࡲ࡛⾑ᾮ࠾ࡼࡧஙỒࢆẖ᪥᥇ྲྀࡋࡓࠋPTU ༊࡛ࡣ⾑୰ T4 ⃰ᗘࢆ 
ᐃࡋࡓࠋஙỒࡣ SCC ࢆ ᐃࡋ, ஙỒ୰ᢠ⳦ᅉᏊ࡜ࡋ࡚ lactoferrin, S100A7, IgA ⃰ᗘࢆ,
chemokine ࡜ࡋ࡚ IL-8 ⃰ᗘࢆ ᐃࡋࡓࠋPTU ༊࡟࠾ࡅࡿ⾑୰ T4 ⃰ᗘࡣ 3 㐌㛫ࡢᢞ୚࡛᭷ព
࡟ῶᑡࡋࡓࠋPTU ༊࠾ࡼࡧ DEX ༊࡟࠾࠸࡚, ࡑࢀࡒࢀࡢ๓ฎ⌮ᮇ㛫๓ᚋ࡛ங୰ SCC,

lactoferrin, S100A7, IgA ࠾ࡼࡧ IL-8 ⃰ᗘ࡟᭷ព࡞ኚ໬ࡣࡳࡽࢀ࡞࠿ࡗࡓࠋLPS ὀධᚋࡢ SCC
ࡣ PTU ༊࠾ࡼࡧ DEX ༊࡟࠾࠸࡚↓ฎ⌮༊࡜ẚ࡭࡚᭷ព࡟ప࠿ࡗࡓࠋࡲࡓ, LPS ὀධᚋࡢங୰
lactoferrin ⃰ᗘࡣ, PTU ༊ࡀ↓ฎ⌮༊࡜ẚ࡭࡚᭷ព࡟ప࠿ࡗࡓࠋS100A7 ⃰ᗘࡣ PTU ༊ࡀ↓ฎ
⌮༊࡟ẚ࡭࡚᭷ព࡟㧗࠿ࡗࡓࠋIgA ⃰ᗘ࠾ࡼࡧ IL-8 ⃰ᗘࡣ༊㛫࡟᭷ព࡞ᕪࡣ࡞࠿ࡗࡓࠋ
 ௨ୖࡢ⤖ᯝ࠿ࡽ, ⏥≧⭢ᶵ⬟పୗ≧ែ࡛ஙᡣෆ࡟ LPS ࢆᢞ୚ࡍࡿ࡜, SCC ࠾ࡼࡧ୍㒊ࡢᢠ
⳦ᅉᏊ⃰ᗘࡢୖ᪼ࡀᢚไࡉࢀࡿ࡜⪃࠼ࡽࢀࡓࠋ
3. ⤖ㄽ
 ᬬ⇕ࢫࢺࣞࢫୗ࡛ࡣ, ೺ᗣ࡞≧ែࡢஙᡣ୍࡛㒊ࡢᢠ⳦ᅉᏊ⃰ᗘࡀపୗࡋ, ࡉࡽ࡟, ᬬ⇕ࢫ
ࢺࣞࢫୗ࡛㉳ࡇࡿ࡜ࡉࢀࡿ⏥≧⭢ᶵ⬟పୗ࠾ࡼࡧ㧗 cortisol ≧ែୗ࡛ࡣ, LPS ὀධᚋ࡟ SCC ࠾
ࡼࡧ୍㒊ࡢᢠ⳦ᅉᏊ⃰ᗘୖ᪼ࡀᢚไࡉࢀࡓࡇ࡜࠿ࡽ, ኟࡢᬬ⇕ᮇ࡟ஙᡣ⅖ࡀከ࠸ࡢࡣ, ங⭢
⮬↛ච␿ᶵ⬟ࡢపୗࡀཎᅉࡢ୍ࡘ࡜⪃࠼ࡽࢀࡓࠋ