ERRγ enhances cardiac maturation with T-tubule formation in human iPSC-derived cardiomyocytes

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Acknowledgements

We thank Drs. Shinya Yamanaka and Seigo Izumo for supporting this project. We also

thank Takako Sono, Ayaka Sakoda, Miyuki Nomura, Yuko Ishikawa, and Haruka

Yamamoto (T-CiRA) for preparing some of the materials and technical assistance, and

Azusa Hama and Yoko Uematsu (CiRA), Shizuka Murakami, Yoshimitsu Nakashima,

Reiko Oikawa (Takeda Pharmaceutical Company Limited), Takanori Matsuo and Aya

Higashide (T-CiRA) for their administrative support. We also thank Peter Karagiannis

(CiRA) for reading the manuscript. We used development samples provided free from

Oji Holdings Corporation for the analysis. This research was supported by the

Takeda–CiRA collaboration program, a grant from Takeda Pharmaceutical Company

Limited, a grant from the Leducq Foundation (18CVD05), a grant from SECOM Science

and Technology Foundation, grants from the Research Center Network for Realization of

Regenerative Medicine (JP19bm0104001, JP19bm0204003, JP19bm0804008, and

JP20bm0804022), Research on Regulatory Science of Pharmaceuticals and Medical

Devices (JP19mk0104117), and Research Project for Practical Applications of Regenerative Medicine (JP19bk0104095) provided by the Japan Agency for Medical Research

and Development, JSPS KAKENHI Grants (JP18K15120, JP18KK0461, JP19K16041 and

JP17H04176) and the iPS Cell Research Fund. Kyoto University and Takeda Pharmaceutical Company Limited have filed a patent application broadly relevant to this work.

Author contributions

K.M., K.D., M.N.-K., N.T., K.I., T.N., and Y.Y. conceived and designed the project. K.M.,

K.D., M.N.-K., S.K., Y.F., M.S., Y.N., I.T. and S.C.N. performed the experimental work.

T.S. and S.I. performed the CRISPR constructions. M.S., A.L.-C., C.O., M.N., T.M., and

T.A. analyzed the RNA-seq data. T.H. and Y.F. performed the transmission electron

microscopy work. K.M., K.D., M.N.-K., A.L.-C., T.N., and Y.Y. wrote the manuscript.

All authors discussed the results.

Competing interests

Y.Y. received research expense from Takeda Pharmaceutical Company, Ltd, and K.D.,

S.K., S.C.N., S.I, T.M., T.A., N.T., K.I., T.N. are employees of Takeda Pharmaceutical

Company, Ltd. K.M., S.K., and Y.Y. are the inventors of the patent application

(WO2019/189554). The other authors declare no competing interests.

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Additional information

Supplementary information The online version contains supplementary material

available at https://doi.org/10.1038/s41467-021-23816-3.

Correspondence and requests for materials should be addressed to T.N. or Y.Y.

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