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大学・研究所にある論文を検索できる 「Influence of human adipose stem cells on prostate cancer cell growth」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。
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Influence of human adipose stem cells on prostate cancer cell growth

Yamamoto, Tokunori Gotoh, Momokazu Koide, Naoshi Funahashi, Yasuhito Shimizu, Shinobu Takei, Yoshifumi 名古屋大学

2020.05

概要

In a novel regenerative cell-based treatment developed by us for the patients with stress urinary incontinence, autologous adipose-derived stem cells (ASCs) are injected into the periurethral region and the external urethral sphincter. Since the candidates for this treatment included prostate cancer patients after radical prostatectomy, we investigated the effects of ASCs on prostate cancer cell proliferation in vitro and in vivo to confirm the feasibility of our therapeutic approach. The LNCaP (human prostate cancer cell line) cells and ASCs were co-cultured, and prostate-specific antigen (PSA) concentration in their culture medium supernatant was measured at 48 and 96 h. The PSA concentration significantly decreased in the coculture medium supernatant as compared to the culture medium with LNCaP cells alone. On the contrary, PSA concentrations in the culture medium of LNCaP cells were not affected by supplementation with ASC culture supernatant. After subcutaneous transplantation of LNCaP cells, with or without ASCs, in immunodeficient mice, tumor growth was compared. The growth of LNCaP xenograft tumor in immunodeficient mice was significantly suppressed by ASC addition. These results indicated that ASCs inhibit prostate cancer cell growth, without no proliferative effect on prostate cancer cells.

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参考文献

1. Fraser JK, Wulur I, Alfonso Z, Hedrick MH. Fat tissue: an underappreciated source of stem cells for

biotechnology. Trends Biotechnol. 2006;24(4):150–154. doi: 10.1016/j.tibtech.2006.01.010.

2. Jiang Y, Jahagirdar BN, Reinhardt RL, et al. Pluripotency of mesenchymal stem cells derived from adult

marrow. Nature. 2002;418(6893):41–49. doi: 10.1038/nature00870.

3. Nagaya N, Kangawa K, Itoh T, et al. Transplantation of mesenchymal stem cells improves cardiac function

in a rat model of dilated cardiomyopathy. Circulation. 2005;112(8):1128–1135. doi: 10.1161/­CIRCULATIO

NAHA.104.500447.

4. Safford KM, Hicok KC, Safford SD, et al. Neurogenic differentiation of murine and human adipose-derived

stromal cells. Biochem Biophys Res Commun. 2002;294(2):371–379. doi: 10.1016/S0006-291X(02)00469-2.

5. Yamamoto T, Gotoh M, Kato M, et al. Periurethral injection of autologous adipose-derived regenerative

cells for the treatment of male stress urinary incontinence: report of three initial cases. Int J Urol.

2012;19(7):652–659. doi: 10.1111/j.1442-2042.2012.02999.x.

6. Gotoh M, Yamamoto T, Kato M, et al. Regenerative treatment of male stress urinary incontinence by

periurethral injection of autologous adipose-derived regenerative cells: 1-year outcomes in 11 patients. Int

J Urol. 2014;21(3):294–300. doi: 10.1111/iju.12266.

7. Gotoh M, Yamamoto T, Shimizu S, et al. Treatment of male stress urinary incontinence using autologous

adipose-derived regenerative cells: long-term efficacy and safety. Int J Urol. 2019;26(3):400–405. doi:

10.1111/iju.13886.

8. Shimizu S, Yamamoto T, Nakayama S, et al. Design of a single-arm clinical trial of regenerative therapy

by periurethral injection of adipose-derived regenerative cells for male stress urinary incontinence in Japan:

the ADRESU study protocol. BMC Urol. 2017;17(1):89. doi: 10.1186/s12894-017-0282-7.

9. Gleave M, Tolcher A, Miyake H, et al. Progression to androgen independence is delayed by adjuvant

treatment with antisense Bcl-2 oligodeoxynucleotides after castration in the LNCaP prostate tumor model.

Clin Cancer Res. 1999;5(10):2891–2898.

10. Xu Q, Wang L, Li H, et al. Mesenchymal stem cells play a potential role in regulating the establishment

and maintenance of epithelial-mesenchymal transition in MCF7 human breast cancer cells by paracrine and

induced autocrine TGF-b. Int J Oncol. 2012;41(3):959–968. doi: 10.3892/ijo.2012.1541.

11. Sakurai M, Miki Y, Takagi K, et al. Interaction with adipocyte stromal cells induces breast cancer malignancy

via S100A7 upregulation in breast cancer microenvironment. Breast Cancer Res. 2017;19(1):70. doi: 10.1186/

s13058-017-0863-0.

12. Li Y, Xu X, Wang L, et al. Senescent mesenchymal stem cells promote colorectal cancer cells growth via

galectin-3 expression. Cell Biosci. 2015;5:21. doi: 10.1186/s13578-015-0012-3.

13. Cousin B, Ravet E, Poglio S, et al. Adult stromal cells derived from human adipose tissue provoke pancreatic

cancer cell death both in vitro and in vivo. PLoS One. 2009;4(7):e6278. doi: 10.1371/journal.pone.0006278.

14. Prantl L, Muehlberg F, Navone NM, et al. Adipose tissue-derived stem cells promote prostate tumor growth.

Prostate. 2010;70(15):1709–1715. doi: 10.1002/pros.21206.

15. Takahara K, Ii M, Inamoto T, et al. Adipose-derived stromal cells inhibit prostate cancer cell proliferation

inducing apoptosis. Biochem Biophys Res Commun. 2014;446(4):1102–1107. doi: 10.1016/j.bbrc.2014.03.080.

16. Takahara K, Ii M, Inamoto T, et al. microRNA-145 mediates the inhibitory effect of adipose tissue-derived

stromal cells on prostate cancer. Stem Cells Dev. 2016;25(17):1290–1298. doi: 10.1089/scd.2016.0093.

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