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Acknowledgements
This work was supported by JST CREST (Grant Number JPMJCR18H5 to S.F.). pGFP-C1-PLCδ-PH and
pmCherry-C1-D4H were kind gifts from Dr. Gregory Fairn (St. Michael’s Hospital, Toronto, Canada)31.
Author contributions
H.H. conceived and directed the project, designed the experiments, established the Piezo1 knockout A431 cells
with the help of Y.H., and analyzed the data. M.K. designed and performed the experiments, and analyzed the
data. T.M., J.V.V.A., and M.I. contributed to analysis for flow cytometry, Rac1 pulldown assay, and plasmid construction for Piezo1 mutants, respectively. M.S. and M.M. performed the scanning electron microscopy observations. Y.H. contributed to construction of Piezo1-related plasmids and establishment of the Piezo1 knockout
cell line. M.K. and H.H. wrote the original manuscript. S.F. supervised the project and wrote the manuscript
with H.H., M.K and M.M. All authors discussed and commented on the manuscript. M.K. and H.H. contributed
equally to this work.
Competing interests The authors declare no competing interests.
Additional information
Supplementary Information The online version contains supplementary material available at https://doi.org/
10.1038/s41598-022-10153-8.
Correspondence and requests for materials should be addressed to H.H. or S.F.
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