Self-assembly of CIP4 drives actin-mediated asymmetric pit-closing in clathrin-mediated endocytosis

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Acknowledgements

We thank T. Ozaki, K. Deguchi, S. Dodo, and B. Yi at Kyoto University,

Graduate School of Biostudies for their technical assistance. We are

especially thankful to Dr. F. Ishidate at the iCeMS Analysis Centre,

Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University Institute for Advanced Study (KUIAS), for his technical assistance

in super-resolution structural illumination microscopy (Elyra 7). We also

thank Mr. Sakai, Mr. Uekusa, Mr. Yagi, and Mr. Ito for their assistance in

constructing and maintaining the HS-AFM system, and Dr. Y. Sasaki at

Kyoto University for their useful discussion on protein multimerisation.

This work was supported by JSPS KAKENHI Grant Numbers JP18H02436,

JP18KK0196, JP19K22422, 19H04830, JP22H05171, and JP23H00369 to

S.H.Y. and AMED (Japan Agency for Medical Research and Development) under Grant Number JP18gm5810018 and JP20wm0325009

to S.H.Y.

Author contributions

Experiments were designed by Y.Y. and S.H.Y. Plasmids construction,

recombinant protein purification, pull-down assay, cell culture and

transfection, live-cell HS-AFM imaging and correlative imaging, live-cell

confocal fluorescence microscopy, super-resolution SIM live-cell imaging, and gel-filtration chromatography were performed by Y.Y. Phase

separation assay and CD spectroscopy were performed by Y.Y. and

S.H.Y. Data analysis was completed by Y.Y. and S.H.Y. Manuscript writing, figure design, and editing was done by Y.Y. and S.H.Y. S.H.Y

supervised and funded the project.

Competing interests

The authors declare no competing interests.

Additional information

Supplementary information The online version contains

supplementary material available at

https://doi.org/10.1038/s41467-023-40390-y.

Correspondence and requests for materials should be addressed to

Shige H. Yoshimura.

Peer review information Nature Communications thanks Grigory

Tagiltsev, Wade Zeno and the other, anonymous, reviewer(s) for their

contribution to the peer review of this work. A peer review file is available.

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