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大学・研究所にある論文を検索できる 「蒸留水による水熱処理がチタン合金の上皮細胞接着性に与える影響」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。
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蒸留水による水熱処理がチタン合金の上皮細胞接着性に与える影響

坂本, 安繁 SAKAMOTO, Yasushige サカモト, ヤスシゲ 九州大学

2021.03.24

概要

Objectives: Enhancement of oral epithelial adhesion to the trans-mucosal material of dental implants may improve their long-term stability. The aim of this study is to investigate whether hydrothermal treatment with distilled water (HT-DW) applied to a Ti-6Al-4V (Ti64) alloy could improve epithelial cellular attachment. We hypothesized that this treatment would enhance the adsorption of proteins and the adhesion of gingival epithelial GE1 cells.

Methods: Ti64 plates were hydrothermally-treated with distilled water at 200°C for 24 h (64HT). After the treatment, the surface characterization was performed. After that, the gingival epithelial GE1 cells were cultured on 64HT or untreated Ti64 plates (64). The number and adhesive strengths of attached cells on each plate were then analyzed.

Results: This treatment changed the surface crystal structure into an anatase type of titanium oxide without an apparent change of surface roughness or topography. Nitrogen was not detected on the 64HT, which indicates decontamination. The 64HT exhibited a hydrophilic surface with a less than 10° angle of water contact. Adsorption of laminin-332 to the 64HT was significantly greater than that of the 64. The number of GE1 cells on the 64HT at 1 and 3 days was significantly lower than that on 64; however, cell adhesion strength on 64HT was greater, with a higher expression of integrin β4, compared with 64.

Conclusions: This indicates that 64HT improves the integration of GE1 cells, which might facilitate the development of a soft tissue barrier around the implant.

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