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大学・研究所にある論文を検索できる 「Silencing of p53 and CDKN1A establishes sustainable immortalized megakaryocyte progenitor cells from human iPSCs」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。
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Silencing of p53 and CDKN1A establishes sustainable immortalized megakaryocyte progenitor cells from human iPSCs

Sone, Masamitsu Nakamura, Sou Umeda, Sachiko Ginya, Harumi Oshima, Motohiko Kanashiro, Maria Alejandra Paul, Sudip Kumar Hashimoto, Kanae Nakamura, Emiri Harada, Yasuo Tsujimura, Kyoko Saraya, Atsunori Yamaguchi, Tomoyuki Sugimoto, Naoshi Sawaguchi, Akira Iwama, Atsushi Eto, Koji Takayama, Naoya 京都大学 DOI:10.1016/j.stemcr.2021.11.001

2021.12

概要

Platelet transfusions are critical for severe thrombocytopenia but depend on blood donors. The shortage of donors and the potential of universal HLA-null platelet products have stimulated research on the ex vivo differentiation of human pluripotent stem cells (hPSCs) to platelets. We recently established expandable immortalized megakaryocyte cell lines (imMKCLs) from hPSCs by transducing MYC, BMI1, and BCL-XL (MBX). imMKCLs can act as cryopreservable master cells to supply platelet concentrates. However, the proliferation rates of the imMKCLs vary with the starting hPSC clone. In this study, we reveal from the gene expression profiles of several MKCL clones that the proliferation arrest is correlated with the expression levels of specific cyclin-dependent kinase inhibitors. Silencing CDKN1A and p53 with the overexpression of MBX was effective at stably inducing imMKCLs that generate functional platelets irrespective of the hPSC clone. Collectively, this improvement in generating imMKCLs should contribute to platelet industrialization and platelet biology.

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