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Supplemental Figure S1
CLP (+)
CD11c
CD11c
CD11c
CD4
CD11c
B220
B220
MLN
B220
CD11c
CD4
PP
CD11c
PLN
B220
B220
B220
B220
CD4
SP
CD11c
CD4
B220
B220
B220
CD4
CD4
B220
B220
B220
CD4
B220
B220
B220
CLP (-)
CD4
CD11c
Supplemental Figure S1. The representative dot-plots are shown for sepsis-induced
changes in ratio of CD4+ T cells, CD11c+ DCs, and B220+ B cells in four different tissues
(SP, MLN, PP, and PLN). For the PLN, inguinal LNs were isolated and used in this assay.
CLP (-) and CLP (+) indicate control steady state and CLP-induced sepsis mice,
respectively. The single-cell suspensions prepared from the tissues of three mice per
group were subjected to antibody staining and flow-cytometry analysis. Data are
representative of at least three independent experiments that show similar results.
Supplemental Figure S2
A (CD3-B220-Gr-1-TER119- cells)
CLP (-)
B (CD3-B220-Gr-1-TER119-CD11c+ cells)
CLP (+)
MHC class II
30000
MHC class II
p < 0.06
MFI
20000
SP
CLP (-)
CLP (+)
10000
MLN
CD11c
SP
MLN
Supplemental Figure S2. Sepsis-induced change in expression of MHC class II on CD3B220-Gr-1-TER119-CD11c+ DCs. (A) Dot plots indicate the representative results of flow
cytometry in which CD3-B220-Gr-1-TER119- cells stained with mAbs to CD11c and MHC
class II were analyzed. (B) The bar graph represents the mean ± SEM for MFI obtained
from four different mice, in which green and purple bars indicate without (-) and with (+)
CLP, respectively. MFI, mean fluorescent intensity. *0.01 < p < 0.05.
Supplemental Figure S3
CD206
FcR1
100
SP MLN
SP MLN
CCR2
MFI
SP MLN
SP MLN
2000
MFI
2000
SP MLN
Ly6c
3000
2000
1000
SP MLN
2000
CLP (-)
CLP (+)
1000
CD11b
6000
CD107b
3000
4000
2000
2000
1000
SP MLN
SP MLN
SP MLN
CD206
FcR1
2000
Ly6c
3000
2000
1000
SP MLN
1000
SP MLN
CCR2
CCR7
2000
1000
SP MLN
100
m"
400
CCR7
200
Lし
800
4000
4000
F4/80
―~
MFI
300
200
200
ーロ ︱
SP MLN
SP MLN
400
MFI
200
MFI
MFI
400
6000
―皿
500
CD107b
600
皿 一屈
訓︱
1000
C (CD3-B220-Gr-1-TER119-CD11c+MHC class II+ cells)
1000
CD11b
2000
ぃ 一し ︱訓 ︱
F4/80
1500
い""
︱いL︱且 ︱
しl
B (CD3-B220-Gr-1-TER119- cells)
CD11c
り︱h
CD3/B220/
Gr-1/TER119
CD3-B220Gr-1-TER119cells
Analysis for
expression of
CD11c+
MHC class II+ inflammatory
DC markers
cells
MHC class II
SSC
SP or MLN cells
SP MLN
CLP (-)
CLP (+)
SP MLN
Supplemental Figure S3. Sepsis-induced change in expression of inflammatory DC
markers. (A) The way of gating the cells for analyzing expression in inflammatory DC
markers is shown. After staining total mononuclear cells from SP or MLN with mAbs to
CD3, B220, Gr-1, TER119, CD11c, and MHC class II, the cells double-positive for CD11c
and MHC class II were gated from CD3-B220-Gr-1-TER119- cells for further analysis.
CD3-B220-Gr-1-TER119- cells (B) and CD3-B220-Gr-1-TER119-CD11c+MHC class II+
cells (C) were analyzed for their expressions of the markers (F4/80, CD11b, CD107b,
FcR1, CD206, or Ly6c) and chemokine receptors (CCR2 and CCR7). The single-cell
suspensions prepared from combined tissues of three mice per group were subjected to
antibody staining and flow-cytometry analysis. Bar graphs represent the mean ± SEM
obtained from two to three independent experiments.
Supplemental Figure S4
% of positive cells
60
% of positive cells
CD3-B220-Gr-1-TER119-CD11c+MHC class II+ cells
60
F4/80
60
60
40
40
40
20
20
20
FcR1
60
CD206
60
40
40
40
20
20
20
SP MLN
60
CCR2
60
40
40
20
20
SP MLN
CD107b
SP MLN
SP MLN
SP MLN
SP MLN
% of positive cells
CD11b
Ly6c
SP MLN
CCR7
CLP (-)
CLP (+)
SP MLN
Supplemental Figure S4. Sepsis-induced change in ratio of inflammatory DC-marker
expressing cells. Ratio of the cells that express the markers (F4/80, CD11b, CD107b,
FcR1, CD206, or Ly6c) and chemokine receptors (CCR2 and CCR7) was measured to
total CD3-B220-Gr-1-TER119-CD11c+MHC class II+ cells. The single-cell suspensions
prepared from combined tissues of three mice per group were subjected to antibody staining
and flow-cytometry analysis. Bar graphs represent the mean ± SEM obtained from two to
three independent experiments.
Supplemental Figure S5
Count
CD4 T cells (isolated)
2.2%
3.0%
2.6%
3.5%
CFSE
CFSE
CFSE
CFSE
10
100
TNF- (ng/mL)
Supplemental Figure S5. TNF- has no effect on CD4 T-cell proliferation. The CD4 T cells
were isolated from single-cell suspensions of SP of Balb/c mice, fluorescently labeled with
CFSE, treated with recombinant mouse TNF- at the indicated concentrations, and further
incubated for 3 days. Flow-cytometry histograms show representative results. The
proliferation ratios were determined via measuring diluted fluorescence intensity of a
histogram in flow cytometry in which the numbers inside squares represent the percentages
of bracketed regions. Data are representative of at least three separate experiments.
Count
Supplemental Figure S6
398
674
10 pg/mL of IL-1
10 ng/mL of IL-1
FoxP3
Supplemental Figure S6. The CD4 T cells proliferated or skewed by IL-1 display a
property of regulatory T cells expressing FoxP3. The CD4 T cells stained with CFSE were
cultured with either 10 pg/mL or 10 ng/mL of IL-1 for 3 days and analyzed for FoxP3
expression in CFSE-diluted cells. Flow cytometry histogram shows change in expression of
FoxP3 in response to treatment with low (10 pg/mL, blue line) or high (10 ng/mL, red line)
doses of IL-1. The numbers inside square represent MFI for FoxP3 expression. MFI, mean
fluorescent intensity. Data are representative of at least three independent experiments.
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