1. Blackwood, E. M. & Eisenman, R. N. Max: A helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex
with Myc. Science 251, 1211–1217 (1991).
2. Rahl, P. B. et al. c-Myc regulates transcriptional pause release. Cell 141, 432–445 (2010).
3. Zhang, K. & Smith, G. W. Maternal control of early embryogenesis in mammals. Reprod. Fertil. Dev. 27, 880–896 (2015).
4. Tadros, W. & Lipshitz, H. D. The maternal-to-zygotic transition: A play in two acts. Development 136, 3033–3042 (2009).
5. Li, L., Zheng, P. & Dean, J. Maternal control of early mouse development. Development 137, 859–870 (2010).
6. Aoki, F., Worrad, D. M. & Schultz, R. M. Regulation of transcriptional activity during the first and second cell cycles in the preimplantation mouse embryo. Dev. Biol. 181, 296–307 (1997).
Scientific Reports |
(2023) 13:16011 |
https://doi.org/10.1038/s41598-023-43127-5
11
Vol.:(0123456789)
www.nature.com/scientificreports/
7. Minami, N., Suzuki, T. & Tsukamoto, S. Zygotic gene activation and maternal factors in mammals. J. Reprod. Dev. 53, 707–715
(2007).
8. Schulz, K. N. & Harrison, M. M. Mechanisms regulating zygotic genome activation. Nat. Rev. Genet. 20, 221–234 (2018).
9. Jukam, D., Shariati, S. A. M. & Skotheim, J. M. Zygotic genome activation in vertebrates. Dev. Cell 42, 316–332 (2017).
10. Zeng, F. & Schultz, R. M. RNA transcript profiling during zygotic gene activation in the preimplantation mouse embryo. Dev. Biol.
283, 40–57 (2005).
11. Suzuki, T., Abe, K. I., Inoue, A. & Aoki, F. Expression of c-MYC in nuclear speckles during mouse oocyte growth and preimplantation development. J. Reprod. Dev. 55, 491–495 (2009).
12. Paria, B. C., Dey, S. K. & Andrews, G. K. Antisense c-myc effects on preimplantation mouse embryo development. Proc. Natl. Acad.
Sci. USA 89, 10051 (1992).
13. Davis, A. C., Wims, M., Spotts, G. D., Hann, S. R. & Bradley, A. A null c-myc mutation causes lethality before 10.5 days of gestation
in homozygotes and reduced fertility in heterozygous female mice. Genes Dev. 7, 671–682 (1993).
14. Asami, M. et al. A program of successive gene expression in mouse one-cell embryos. Cell Rep. 42, 112023 (2023).
15. Kinisu, M. et al. Klf5 establishes bi-potential cell fate by dual regulation of ICM and TE specification genes. Cell Rep. 37, 109982
(2021).
16. Aoki, F. Zygotic gene activation in mice: Profile and regulation. J. Reprod. Dev. 68, 79 (2022).
17. Gambini, A. et al. Developmentally programmed tankyrase activity upregulates β-catenin and licenses progression of embryonic
genome activation. Dev. Cell 53, 545-560.e7 (2020).
18. Krepelova, A., Neri, F., Maldotti, M., Rapelli, S. & Oliviero, S. Myc and max genome-wide binding sites analysis links the Myc
regulatory network with the polycomb and the core pluripotency networks in mouse embryonic stem cells. PLoS ONE 9, 88933
(2014).
19. Arand, J. et al. Tet enzymes are essential for early embryogenesis and completion of embryonic genome activation. EMBO Rep.
23, e53968 (2022).
20. Park, S. J., Shirahige, K., Ohsugi, M. & Nakai, K. DBTMEE: A database of transcriptome in mouse early embryos. Nucleic Acids
Res. 43, D771 (2015).
21. Malynn, B. A. et al. N-myc can functionally replace c-myc in murine development, cellular growth, and differentiation. Genes Dev.
14, 1390 (2000).
22. Müller, I. et al. Targeting of the MYCN protein with small molecule c-MYC inhibitors. PLoS ONE 9, e97285 (2014).
23. Fletcher, S. & Prochownik, E. V. Small-molecule inhibitors of the Myc oncoprotein. Biochim. Biophys. Acta Gene Regul. Mech.
1849, 525–543 (2015).
24. Abe, K. et al. Minor zygotic gene activation is essential for mouse preimplantation development. Proc. Natl. Acad. Sci. 115, E6780–
E6788 (2018).
25. Lin, C. Y. et al. Transcriptional amplification in tumor cells with elevated c-Myc. Cell 151, 56 (2012).
26. Fernandez, P. C. et al. Genomic targets of the human c-Myc protein. Genes Dev. 17, 1115 (2003).
27. Dang, C. V. MYC on the path to cancer. Cell 149, 22 (2012).
28. Takahashi, K. & Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined
factors. Cell 126, 663–676 (2006).
29. Fu, X., Wu, X., Djekidel, M. N. & Zhang, Y. Myc and Dnmt1 impede the pluripotent to totipotent state transition in embryonic
stem cells. Nat. Cell Biol. 21, 835 (2019).
30. AVMA Guidelines for the Euthanasia of Animals. https://www.avma.org/sites/default/files/2020-02/Guidelines-on-Euthanasia-
2020.pdf (2020).
31. Minami, N., Sasaki, K., Aizawa, A., Miyamoto, M. & Imai, H. Analysis of gene expression in mouse 2-cell embryos using fluorescein
differential display: Comparison of culture environments. Biol. Reprod. 64, 30–35 (2001).
32. Ho, Y., Wigglesworth, K., Eppig, J. J. & Schultz, R. M. Preimplantation development of mouse embryos in KSOM: Augmentation
by amino acids and analysis of gene expression. Mol. Reprod. Dev. 41, 232–238 (1995).
33. Shikata, D., Yamamoto, T., Honda, S., Ikeda, S. & Minami, N. H4K20 monomethylation inhibition causes loss of genomic integrity
in mouse preimplantation embryos. J. Reprod. Dev. 66, 411–419 (2020).
34. Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method.
Methods 25, 402–408 (2001).
35. Yin, X., Giap, C., Lazo, J. S. & Prochownik, E. V. Low molecular weight inhibitors of Myc-Max interaction and function. Oncogene
22, 6151–6159 (2003).
36. Langmead, B. & Salzberg, S. L. Fast gapped-read alignment with Bowtie 2. Nat. Methods 9, 357–359 (2012).
37. Li, H. et al. The sequence alignment/map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).
38. Zhang, Y. et al. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 9, 1–9 (2008).
39. Dobin, A. et al. STAR: Ultrafast universal RNA-seq aligner. Bioinformatics 29, 15 (2013).
40. Li, B. & Dewey, C. N. RSEM: Accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC
Bioinform. 12, 323 (2011).
41. Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome
Biol. 15, 550 (2014).
42. Huang, D. W., Sherman, B. T. & Lempicki, R. A. Systematic and integrative analysis of large gene lists using DAVID bioinformatics
resources. Nat. Protoc. 4, 44–57 (2009).
43. Huang, D. W., Sherman, B. T. & Lempicki, R. A. Bioinformatics enrichment tools: Paths toward the comprehensive functional
analysis of large gene lists. Nucleic Acids Res. 37, 1 (2009).
Acknowledgements
This work was supported by a Grant-in-Aid for Scientific Research (no. 19H03136 to NM) and a Grant-in-Aid
for JSPS Fellows (no. 21J21840 to TY) from the Japan Society for the Promotion of Science.
Author contributions
T.Y., H.W., S.H., S.I., and N.M. conceived the study. T.Y., H.W., and H.S. performed the experiments and analyzed
the data. T.Y., S.H., S.I., and N.M. wrote the manuscript. All the authors discussed the results and approved the
final manuscript.
Competing interests The authors declare no competing interests.
Additional information
Correspondence and requests for materials should be addressed to N.M.
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