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謝辞
本研究は、京都大学大学院生命科学研究科 高次生命科学専攻 脳機能発達再生制御 今
吉研究室にて実施させて頂きました。今吉格教授と議論を重ねることで、数多くの知
見を深めることができました。また実験技術をご指導して頂いたことに加え、自身の
研究アイデアを積極的に試すことができる素晴らしい環境を与えてくださったこと
に、心より御礼申し上げます。山田真弓講師 (現・京都大学 医生物学研究所、特定准
教授) には、数多くの研究手法やアドバイスを親切丁寧にご指導していただきまし
た。今後の研究活動の基盤となる技術を伝授して頂いたことを深く感謝致します。ま
た、鈴木裕輔助教には、光作動性転写因子の時間的な定量に関してサポートして頂
き、GUY Adam Tsuda 准教授には、ニワトリ胚における eGAV の遺伝子導入に関
してサポートして頂きました。eGAV-eMag の実験では、福田智徳大学院生にサポー
トして頂きました。本研究の迅速な遂行にご助力頂いたことを深く感謝致します。
今吉研究室と影山研究室 (元・医生物学研究所、現・理化学研究所 脳神経科学研究セ
ンター) のメンバーと定期的に議論させていただいたことは、自身の研究を客観的に
捉える機会に繋がりました。また、京都大学大学院 生命科学研究科/医学研究科 松田
道行教授、寺井健太准教授 (現・京都大学 医学研究科、准教授)、小鉢健樹大学院
生、基礎生物学研究所 定量生物学研究部門 青木一洋教授、宇田耀一博士、理化学研
究所 脳神経科学研究センター 宮脇敦史教授には、本研究の迅速な遂行にご助力頂き
ました。深く感謝致します。
本研究は、日本学術振興会特別研究員制度 (DC1) のご支援を賜りましたこと、深く
感謝申し上げます。
最後になりましたが、本研究に携わってくださった皆様に心より感謝申し上げます。
本学位論文は、以下の学術論文の内容に基づいて書かれたものである。
NAGASAKI, S. C., FUKUDA, T. D., YAMADA, M., SUZUKI, Y., III, KAKUTANI,
R., GUY, A. T. & IMAYOSHI, I. 2023. Enhancement of Vivid-based photoactivatable Gal4 transcription factor in mammalian cells. Cell Structure and
Function, 48, 31-47.
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