Aguzzi, A., and Altmeyer, M. (2016). Phase Separation: Linking Cellular Compartmentalization to Disease. Trends Cell Biol. 26, 547–558. de Anda, F.C., Pollarolo, G., Da Silva, J.S., Camoletto, P.G., Feiguin, F., and Dotti, C.G. (2005). Centrosome localization determines neuronal polarity. Nature 436, 704–708.
Arquint, C., and Nigg, E.A. (2016). The PLK4-STIL-SAS-6 module at the core of centriole duplication. Biochem. Soc. Trans. 44, 1253–1263.
Arquint, C., Sonnen, K.F., Stierhof, Y.-D., and Nigg, E.A. (2012). Cellcycle-regulated expression of STIL controls centriole number in human cells. J. Cell Sci. 125, 1342–1352.
Aydogan, M.G., Wainman, A., Saurya, S., Steinacker, T.L., Caballe, A., Novak, Z.A., Baumbach, J., Muschalik, N., and Raff, J.W. (2018). A homeostatic clock sets daughter centriole size in flies. J. Cell Biol. 217, 1233–1248.
Banani, S.F., Lee, H.O., Hyman, A.A., and Rosen, M.K. (2017).
Biomolecular condensates: organizers of cellular biochemistry. Nat. Rev. Mol. Cell Biol. 18, 285–298.
Bauer, M., Cubizolles, F., Schmidt, A., and Nigg, E.A. (2016). Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging. EMBO J. 35, 2152-2166.
Bettencourt-Dias, M., Rodrigues-Martins, A., Carpenter, L., Riparbelli, M.,Lehmann, L., Gatt, M.K., Carmo, N., Balloux, F., Callaini, G., and Glover,D.M. (2005). SAK/PLK4 Is Required for Centriole Duplication and Flagella Development. Curr. Biol. 15, 2199–2207.
Boeynaems, S., Alberti, S., Fawzi, N.L., Mittag, T., Polymenidou, M.,Rousseau, F., Schymkowitz, J., Shorter, J., Wolozin, B., Bosch, L. Van Den, et al. (2018a). Protein Phase Separation: A New Phase in Cell Biology. Trends Cell Biol. 28, 420–435.
Boeynaems, S., Alberti, S., Fawzi, N.L., Mittag, T., Polymenidou, M., Rousseau, F., Van, L., Bosch, D., Tompa, P., and Fuxreiter, M. (2018b). Protein Phase Separation: A New Phase in Cell Biology.
Bornens, M. (2012). The centrosome in cells and organisms. Science 335,422–426.
Boveri T. (1888). Die Befruchtung und Teilung des Eies von Ascaris megalocephala. Zellen-Studien 2. Jena: G. Fischer
Boveri T. )1900). Ueber die Natur der Centrosomen. Zellen-Studien 4. Jena: G. Fischer
Carpenter, K., Bell, R.B., Yunus, J., Amon, A., and Berchowitz, L.E. (2018). Phosphorylation-Mediated Clearance of Amyloid-like Assemblies in Meiosis. Dev. Cell 45, 392–405.e6.
Carvalho-Santos, Z., Machado, P., Branco, P., Tavares-Cadete, F., Rodrigues-Martins, A., Pereira-Leal, J.B., and Bettencourt-Dias, M. (2010).Stepwise evolution of the centriole-assembly pathway. J. Cell Sci. 123,1414–1426.
Cunha-Ferreira, I., Rodrigues-Martins, A., Bento, I., Riparbelli, M., Zhang, W., Laue, E., Callaini, G., Glover, D.M., and Bettencourt-Dias, M. (2009). The SCF/Slimb Ubiquitin Ligase Limits Centrosome Amplification through Degradation of SAK/PLK4. Curr. Biol. 19, 43–49.
Cunha-Ferreira, I., Bento, I., Pimenta-Marques, A., Jana, S.C., Lince-Faria, M., Duarte, P., Borrego-Pinto, J., Gilberto, S., Amado, T., Brito, D., et al. (2013). Regulation of Autophosphorylation Controls PLK4 SelfDestruction and Centriole Number. Curr. Biol. 23, 2245–2254.
Delattre, M., Leidel, S., Wani, K., Baumer, K., Bamat, J., Schnabel, H., Feichtinger, R., Schnabel, R., and Gönczy, P. (2004). Centriolar SAS-5 is required for centrosome duplication in C. elegans. Nat. Cell Biol. 6, 656–664.
Dzhindzhev, N.S., Tzolovsky, G., Lipinszki, Z., Schneider, S., Lattao, R., Fu, J., Debski, J., Dadlez, M., and Glover, D.M. (2014). Plk4 Phosphorylates Ana2 to Trigger Sas6 Recruitment and Procentriole Formation. Curr. Biol. 24, 2526–2532.
Dzhindzhev, N.S., Tzolovsky, G., Lipinszki, Z., Abdelaziz, M., Debski, J., Dadlez, M., and Glover, D.M. (2017). Two-step phosphorylation of Ana2 by Plk4 is required for the sequential loading of Ana2 and Sas6 to initiate procentriole formation. Open Biol. 7.
Feric, M., Vaidya, N., Harmon, T.S., Mitrea, D.M., Zhu, L., Richardson, T.M., Kriwacki, R.W., Pappu, R. V., and Brangwynne, C.P. (2016). Coexisting Liquid Phases Underlie Nucleolar Subcompartments. Cell 165, 1686–1697.
Franzmann, T.M., Jahnel, M., Pozniakovsky, A., Mahamid, J., Holehouse, A.S., Nüske, E., Richter, D., Baumeister, W., Grill, S.W., Pappu, R. V, et al. (2018). Phase separation of a yeast prion protein promotes cellular fitness. Science 359, eaao5654.
Godinho, S.A., Picone, R., Burute, M., Dagher, R., Su, Y., Leung, C.T., Polyak, K., Brugge, J.S., Théry, M., and Pellman, D. (2014). Oncogenelike induction of cellular invasion from centrosome amplification. Nature 510, 167–171.
Gönczy, P., Echeverri, C., Oegema, K., Coulson, A., Jones, S.J.M., Copley, R.R., Duperon, J., Oegema, J., Brehm, M., Cassin, E., et al. (2000). Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III. Nature 408, 331–336.
Goryachev, A.B., and Leda, M. (2017). Many roads to symmetry breaking: molecular mechanisms and theoretical models of yeast cell polarity. Mol. Biol. Cell 28, 370–380.
Guderian, G., Westendorf, J., Uldschmid, A., and Nigg, E.A. (2010). Plk4 trans-autophosphorylation regulates centriole number by controlling betaTrCP-mediated degradation. J. Cell Sci. 123, 2163–2169.
Guillén-Boixet, J., Buzon, V., Salvatella, X., and Méndez, R. (2016). CPEB4 is regulated during cell cycle by ERK2/Cdk1-mediated phosphorylation and its assembly into liquid-like droplets. Elife 5, e19298.
Habedanck, R., Stierhof, Y.-D., Wilkinson, C.J., and Nigg, E.A. (2005). The Polo kinase Plk4 functions in centriole duplication. Nat. Cell Biol. 7, 1140–1146.
Holland, A.J., Lan, W., Niessen, S., Hoover, H., and Cleveland, D.W. (2010). Polo-like kinase 4 kinase activity limits centrosome overduplication by autoregulating its own stability. J. Cell Biol. 188, 191–198.
Holland, A.J., Fachinetti, D., Zhu, Q., Bauer, M., Verma, I.M., Nigg, E.A. and Cleaveland, D.W. (2012). The autoregulated instability of Poli-like kinase 4 limits centrosome duplication to once per cell cycle. Genes Dev. 26, 2684-9.
Ishikawa, H., Kubo, A., Tsukita, S., and Tsukita, S. (2005). Odf2-deficient mother centrioles lack distal/subdistal appendages and the ability to generate primary cilia. Nat. Cell Biol. 7, 517–524.
Keller, D., Orpinell, M., Olivier, N., Wachsmuth, M., Mahen, R., Wyss, R., Hacher, V., Ellenberg, J., Manley, S. and Gönczy, P. (2014). Mechanisms of HsSAS6 assembly promoting centriole formation in human cells. J Cell Biol. 204, 697-712.
Kim, T.-S., Park, J.-E., Shukla, A., Choi, S., Murugan, R.N., Lee, J.H., Ahn, M., Rhee, K., Bang, J.K., Kim, B.Y., et al. (2013). Hierarchical recruitment of Plk4 and regulation of centriole biogenesis by two centrosomal scaffolds, Cep192 and Cep152. Proc. Natl. Acad. Sci. U. S. A. 110, E4849-57.
Klebba, J.E., Buster, D.W., Nguyen, A.L., Swatkoski, S., Gucek, M., Rusan, N.M., and Rogers, G.C. (2013). Polo-like Kinase 4 Autodestructs by Generating Its Slimb-Binding Phosphodegron. Curr. Biol. 23, 2255–2261.
Klebba, J.E., Buster, D.W., McLamarrah, T.A., Rusan, N.M., and Rogers, G.C. (2015). Autoinhibition and relief mechanism for Polo-like kinase 4. Proc. Natl. Acad. Sci. U. S. A. 112, E657-66.
Kleylein-Sohn, J., Westendorf, J., Le Clech, M., Habedanck, R., Stierhof, Y.-D., and Nigg, E.A. (2007). Plk4-Induced Centriole Biogenesis in Human Cells. Dev. Cell 13, 190–202.
Kondo, S., and Miura, T. (2010). Reaction-diffusion model as a framework for understanding biological pattern formation. Science 329, 1616–1620.
Kratz, A.-S., Bärenz, F., Richter, K.T., and Hoffmann, I. (2015). Plk4- dependent phosphorylation of STIL is required for centriole duplication. Biol. Open 4, 370–377.
Kumar, A., Girimaji, S.C., Duvvari, M.R., and Blanton, S.H. (2009). Mutations in STIL, Encoding a Pericentriolar and Centrosomal Protein,Cause Primary Microcephaly. Am. J. Hum. Genet. 84, 286–290. van der Lee, R., Buljan, M., Lang, B., Weatheritt, R.J., Daughdrill, G.W., Dunker, A.K., Fuxreiter, M., Gough, J., Gsponer, J., Jones, D.T., et al. (2014). Classification of intrinsically disordered regions and proteins. Chem. Rev. 114, 6589–6631.
Leidel, S., Delattre, M., Cerutti, L., Baumer, K., and Gönczy, P. (2005). SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. Nat. Cell Biol. 7, 115–125.
Levine, M.S., Bakker, B., Boeckx, B., Moyett, J., Lu, J., Vitre, B., Spierings, D.C., Lansdorp, P.M., Cleveland, D.W., Lambrechts, D., et al. (2017). Centrosome Amplification Is Sufficient to Promote Spontaneous Tumorigenesis in Mammals. Dev. Cell 40, 313–322.e5.
Li, R., and Bowerman, B. (2010). Symmetry breaking in biology. Cold Spring Harb. Perspect. Biol. 2, a003475.
Li, J., Tan, M., Li, L., Pamarthy, D., Lawrence, T.S., and Sun, Y. (2005). SAK, A New Polo-Like Kinase, Is Transcriptionally Repressed by p53 and Induces Apoptosis upon RNAi Silencing. Neoplasia 7, 312–323.
Li, Z., Dai, K., Wang, C., Song, Y., Gu, F., Liu, F., and Fu, L. (2016). Expression of Polo-Like Kinase 4(PLK4) in Breast Cancer and Its Response to Taxane-Based Neoadjuvant Chemotherapy. J. Cancer 7, 1125–1132.
Lopes, C.A.M., Jana, S.C., Cunha-Ferreira, I., Zitouni, S., Bento, I., Duarte, P., Gilberto, S., Freixo, F., Guerrero, A., Francia, M., et al. (2015). PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space. Dev. Cell 35,222–235.
Martin, S.G. (2015). Spontaneous cell polarization: Feedback control of Cdc42 GTPase breaks cellular symmetry. BioEssays 37, 1193–1201.
Martin, C.-A., Ahmad, I., Klingseisen, A., Hussain, M.S., Bicknell, L.S., Leitch, A., Nürnberg, G., Toliat, M.R., Murray, J.E., Hunt, D., et al. (2014).
Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. Nat. Genet. 46, 1283–1292.
McLamarrah, T.A., Buster, D.W., Galletta, B.J., Boese, C.J., Ryniawec, J.M., Hollingsworth, N.A., Byrnes, A.E., Brownlee, C.W., Slep, K.C., Rusan, N.M., et al. (2018). An ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly. J. Cell Biol. 217, 1217–1231.
Mitrea, D.M., and Kriwacki, R.W. (2016). Phase separation in biology; functional organization of a higher order. Cell Commun. Signal. 14, 1.
Monahan, Z., Ryan, V.H., Janke, A.M., Burke, K.A., Rhoads, S.N., Zerze, G.H., O’Meally, R., Dignon, G.L., Conicella, A.E., Zheng, W., et al. (2017). Phosphorylation of the FUS low-complexity domain disrupts phase separation, aggregation, and toxicity. EMBO J. 36, 2951–2967.
Moyer, T.C., Clutario, K.M., Lambrus, B.G., Daggubati, V., and Holland, A.J. (2015). Binding of STIL to Plk4 activates kinase activity to promote centriole assembly. J. Cell Biol. 209, 863–878.
Murray, D.T., Kato, M., Lin, Y., Thurber, K.R., Hung, I., McKnight, S.L., and Tycko, R. (2017). Structure of FUS Protein Fibrils and Its Relevance to Self-Assembly and Phase Separation of Low-Complexity Domains. Cell 171, 615–627.e16.
Nigg, E.A., and Raff, J.W. (2009). Centrioles, Centrosomes, and Cilia in Health and Disease. Cell 139, 663–678.
Nott, T.J., Petsalaki, E., Farber, P., Jervis, D., Fussner, E., Plochowietz, A., Craggs, T.D., Bazett-Jones, D.P., Pawson, T., Forman-Kay, J.D., et al. (2015). Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles. Mol. Cell 57, 936–947.
O’Connell, K.F., Caron, C., Kopish, K.R., Hurd, D.D., Kemphues, K.J., Li, Y., and White, J.G. (2001). The C. elegans zyg-1 Gene Encodes a Regulator of Centrosome Duplication with Distinct Maternal and Paternal Roles in the Embryo. Cell 105, 547–558.
Ohta, M., Ashikawa, T., Nozaki, Y., Kozuka-Hata, H., Goto, H., Inagaki, M., Oyama, M., and Kitagawa, D. (2014). Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole. Nat. Commun. 5, 5267.
Ohta, M., Watanabe, K., Ashikawa, T., Nozaki, Y., Yoshiba, S., Kimura, A., and Kitagawa, D. (2018). Bimodal Binding of STIL to Plk4 Controls Proper Centriole Copy Number. Cell Rep. 23, 3160–3169.e4.
Park, S.-Y., Park, J.-E., Kim, T.-S., Kim, J.H., Kwak, M.-J., Ku, B., Tian, L., Murugan, R.N., Ahn, M., Komiya, S., et al. (2014). Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nat. Struct. Mol. Biol. 21, 696–703.
Peel, N., Stevens, N.R., Basto, R., and Raff, J.W. (2007). Overexpressing Centriole-Replication Proteins In Vivo Induces Centriole Overduplication and De Novo Formation. Curr. Biol. 17, 834–843.
Pelletier, L., O’Toole, E., Schwager, A., Hyman, A.A., and MüllerReichert, T. (2006). Centriole assembly in Caenorhabditis elegans. Nature 444, 619–623.
Rodrigues-Martins, A., Riparbelli, M., Callaini, G., Glover, D.M., and Bettencourt-Dias, M. (2008). From centriole biogenesis to cellular function: Centrioles are essential for cell division at critical developmental stages. Cell Cycle 7, 11–16.
Saad, S., Cereghetti, G., Feng, Y., Picotti, P., Peter, M., and Dechant, R. (2017). Reversible protein aggregation is a protective mechanism to ensure cell cycle restart after stress. Nat. Cell Biol. 19, 1202–1213.
Sabari, B.R., Dall’Agnese, A., Boija, A., Klein, I.A., Coffey, E.L., Shrinivas, K., Abraham, B.J., Hannett, N.M., Zamudio, A. V, Manteiga, J.C., et al. (2018). Coactivator condensation at super-enhancers links phase separation and gene control. Science 361, eaar3958.
Scheer, U. (2014). Historical roots of centrosome research: discovery of Boveri’s microscope slides in Würzburg. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 369.
Sheu-Gruttadauria, J., and MacRae, I.J. (2018). Phase Transitions in the Assembly and Function of Human miRISC. Cell 173, 946–957.e16.
Shin, Y., and Brangwynne, C.P. (2017). Liquid phase condensation in cell physiology and disease. Science (80-. ). 357, eaaf4382.
Sillibourne, J.E., Tack, F., Vloemans, N., Boeckx, A., Thambirajah, S., Bonnet, P., Ramaekers, F.C.S., Bornens, M., and Grand-Perret, T. (2010). Autophosphorylation of polo-like kinase 4 and its role in centriole duplication. Mol. Biol. Cell 21, 547–561.
Slevin, L.K., Nye, J., Pinkerton, D.C., Buster, D.W., Rogers, G.C., and Slep, K.C. (2012). The structure of the plk4 cryptic polo box reveals two tandem polo boxes required for centriole duplication. Structure 20, 1905–1917.
Sonnen, K.F., Gabryjonczyk, A.-M., Anselm, E., Stierhof, Y.-D., and Nigg, E.A. (2013). Human Cep192 and Cep152 cooperate in Plk4 recruitment and centriole duplication. J. Cell Sci. 126, 3223–3233.
Sönnichsen, B., Koski, L.B., Walsh, A., Marschall, P., Neumann, B., Brehm, M., Alleaume, A.-M., Artelt, J., Bettencourt, P., Cassin, E., et al. (2005). Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans. Nature 434, 462–469.
Stinchcombe, J.C., Majorovits, E., Bossi, G., Fuller, S., and Griffiths, G.M. (2006). Centrosome polarization delivers secretory granules to the immunological synapse. Nature 443, 462–465.
Strnad, P., Leidel, S., Vinogradova, T., Euteneuer, U., Khodjakov, A., and Gönczy, P. (2007). Regulated HsSAS-6 Levels Ensure Formation of a
Single Procentriole per Centriole during the Centrosome Duplication Cycle. Dev. Cell 13, 203–213.
Su, X., Ditlev, J.A., Hui, E., Xing, W., Banjade, S., Okrut, J., King, D.S., Taunton, J., Rosen, M.K., and Vale, R.D. (2016). Phase separation of signaling molecules promotes T cell receptor signal transduction. Science 352, 595–599.
Takao, D., Yamamoto, S., and Kitagawa, D. (2018). A theory of centriole duplication based on self-organized spatial pattern formation. BioRxiv 424754.
Torii, K.U. (2012). Two-dimensional spatial patterning in developmental systems. Trends Cell Biol. 22, 438–446.
Vidwans, S.J., Wong, M.L., and O’Farrell, P.H. (1999). Mitotic Regulators Govern Progress through Steps in the Centrosome Duplication Cycle. J. Cell Biol. 147, 1371–1378.
Vorobjev, I.A., and Chentsov YuS, C. (1982). Centrioles in the cell cycle. I. Epithelial cells. J. Cell Biol. 93, 938–949.
Wang, J., Choi, J.-M., Holehouse, A.S., Lee, H.O., Zhang, X., Jahnel, M., Maharana, S., Lemaitre, R., Pozniakovsky, A., Drechsel, D., et al. (2018). A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins. Cell 174, 688–699.e16.
Wang, J.T., Smith, J., Chen, B.-C., Schmidt, H., Rasoloson, D., Paix, A., Lambrus, B.G., Calidas, D., Betzig, E., and Seydoux, G. (2014). Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans. Elife 3, e04591.
Woodruff, J.B., Ferreira Gomes, B., Widlund, P.O., Mahamid, J., Honigmann, A., and Hyman, A.A. (2017). The Centrosome Is a Selective Condensate that Nucleates Microtubules by Concentrating Tubulin. Cell 169, 1066–1077.e10.
Woodruff, J.B., Hyman, A.A., and Boke, E. (2018). Organization and Function of Non-dynamic Biomolecular Condensates. Trends Biochem. Sci. 43, 81–94.
Zitouni, S., Nabais, C., Jana, S.C., Guerrero, A., and Bettencourt-Dias, M. (2014). Polo-like kinases: structural variations lead to multiple functions. Nat. Rev. Mol. Cell Biol. 15, 433–452.