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SUPPLEMENTARY MATERIAL ONLINE
Supplementary materials and methods
Figure S1. Immunohistochemical staining in early oesophageal squamous cell carcinoma (ESCC) tissues using normal rabbit IgG, anti-integrin β4 (β4)
antibody, and anti-β4 antibody with β4-blocking peptide
Figure S2. Characteristics of various polarised peripheral blood monocyte-derived macrophages
Figure S3. Phosphokinase expression profile of the protein from Het-1A monoculture and Het-1A co-cultured with M0 macrophages (co-M0), M1
macrophages (co-M1), and M2 macrophages (co-M2)
Figure S4. Cytokine expression profile of the condition medium from Het-1A and M2 macrophages (M2) monoculture and Het-1A co-cultured
with M2
© 2023 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd
on behalf of The Pathological Society of Great Britain and Ireland. www.pathsoc.org
J Pathol 2023; 261: 55–70
www.thejournalofpathology.com
10969896, 2023, 1, Downloaded from https://pathsocjournals.onlinelibrary.wiley.com/doi/10.1002/path.6148 by Kobe University, Wiley Online Library on [03/09/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
Role of macrophages in early oesophageal squamous cell carcinoma
S Urakami et al
Figure S5. YKL-40/osteopontin (OPN) promoted the proliferation and migration of oesophageal epithelial cells via the mTOR–p70S6K signalling
pathway
Figure S6. YKL-40 and osteopontin (OPN) promoted the proliferation and migration of Het-1A, TE-9, and TE-11 cells via the mTOR–p70S6K
signalling pathway
Figure S7. The co-localization between YKL-40 or osteopontin (OPN) and integrin β4 (β4) and intensity of β4 on Het-1A cells co-cultured with M2
macrophages (M2) for 24 h were increased compared with monocultured Het-1A cells
Figure S8. YKL-40/osteopontin (OPN) promoted the proliferation and migration of oesophageal epithelial cells by activating the mTOR–p70S6K
signalling pathway via integrin β4 (β4)
Figure S9. Immunohistochemical analysis of infiltrating immune cells and tumour cells in oesophagus tissues of the mouse oesophageal carcinogens
model
Figure S10. Immunohistochemical analysis of infiltrating immune cells and tumour cells in early oesophageal squamous cell carcinoma (ESCC) tissues
Figure S11. Quantification of western blotting bands on the mTOR–p70S6K signalling pathway
Table S1. Correlation between the expression levels of integrin β4 (β4) and phosphorylated p70S6K (p-p70S6K) and infiltrating cells in oesophageal
squamous cell carcinoma (ESCC) tissues
Table S2. Correlation between the numbers of infiltrating YKL-40- and osteopontin (OPN)-positive cells and clinicopathological parameters in early
oesophageal squamous cell carcinoma (ESCC)
Table S3. Characteristics of patients and oesophageal squamous cell carcinoma (ESCC) tissues (referred to in Supplementary materials and methods)
Table S4. Experimental materials and the primer sets used for RT-qPCR (referred to in Supplementary materials and methods)
© 2023 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd
on behalf of The Pathological Society of Great Britain and Ireland. www.pathsoc.org
J Pathol 2023; 261: 55–70
www.thejournalofpathology.com
10969896, 2023, 1, Downloaded from https://pathsocjournals.onlinelibrary.wiley.com/doi/10.1002/path.6148 by Kobe University, Wiley Online Library on [03/09/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
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