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Figure legends

Fig. 1. Cryostat section of M. vastus medialis. The muscle fibers of the transverse section reveal

varying in size and round shape with internal nuclei. Endomysial connective tissue and number of

satellite nuclei are increased. HE.

Fig. 2. Cryostat section of M. vastus medialis. The muscle fibers of the longitudinal section

show nuclear chains in the mid-portion of the fiber. Increased numbers of satellite nuclei are also

visible. HE.

Fig. 3.

M. longissimus lumborum stained with Gomori’s trichrome shows variable numbers of

nemaline rods. Gomori’s trichrome staining.

Fig. 4. The longitudinal section of M. longissimus lumborum reveals the nemaline rods arise from

the Z disk (arrows). Numerous nemaline rods are observed in the center of fibers, associated with

or formed myofibrillar degeneration. Bar: 10μm.

Fig. 1

Fig. 3

Fig. 2

Fig. 4

Supplementary data-1

fig. 1

fig. 2

fig. 3

fig. 4

fig. 1. Gross features of the muscles of the hind legs. M. vastus medialis reveals pale (arrows), while

the semimembranosus muscle (arrow heads) shows relatively normal color.

fig. 2. Cryostat section of M. semimembranosus from control animal. Normal cytoplasmic staining is

pale green, and nuclei and sites of high mitochondrial density stain red. Modified Gomori’s trichrome.

fig. 3. Cryostat section of M. semimembranosus from control animal. NADH-TR staining shows a

ranom distribution of the fibers with high and low activity depend on the presence of oxidative

activity.

fig. 4. Cryostat section demonstrates mild lesion and varying fiber size with internal nuclei in M.

semimembranosus. HE.

Supplementary data-2

fig. 5

fig. 6

fig. 5. Cryostat section of M. vastus medialis shows severe lesion associated with fat tissue

infiltration. HE.

fig. 6. Staining for NADH-TR reveals fibers with sarcoplasmic strands radiating from the central

nucleus (arrows) and increased reactivity showing ring-like appearance (arrow heads). NADH-TR.

Supplementary data-3

fig. 7a

fig. 7b

fig. 8

fig. 7a, b. The diaphragmatic muscles are consisted of small rounded or polygonal fibers with

several internal nuclei (a). NADH-TR staining reveals the fibers with a dark central region

surrounded by pale peripheral halo (b). a: HE, b: NADH-TR.

fig. 8. M. longissimus lumborum shows variable numbers of nemaline rods revealed strong

immunoreactivity with α–actinin antibody. IHC.

Supplementary data-4

fig. 9

fig. 10

fig.9. The immature fiber of the diaphragmatic muscles shows the centrally placed nuclei

surrounded by glycogen granules, and a reduction in myofibrils. Inset shows the organelles

probably originating from the mitochondria. Bar: 10μm.

fig.10. Rnig-like or necklace-like fiber of M. vastus medialis reveals the center bordered by an

area devoid of myofibrils and containing glycogen granules and dilated sarcoplasmic reticulum.

Peripheral area shows a zone with lack of myofibrils. Inset shows dilated sarcoplasmic

reticulum. Bar: 10μm.

...