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大学・研究所にある論文を検索できる 「Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

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Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris

Ito, Yoichiro Ishigami, Misa Hashiba, Noriko Nakamura, Yasuyuki Terai, Goro Hasunuma, Tomohisa Ishii, Jun Kondo, Akihiko 神戸大学

2022.09.01

概要

Summary In our previous study, we serendipitously discovered that protein secretion in the methylotrophic yeast Pichia pastoris is enhanced by a mutation (V50A) in the mating factor alpha (MFα) prepro-leader signal derived from Saccharomyces cerevisiae. In the present study, we investigated 20 single-amino-acid substitutions, including V50A, located within the MFα signal peptide, indicating that V50A and several single mutations alone provided significant increase in production of the secreted proteins. In addition to hydrophobicity index analysis, both an unfolded protein response (UPR) biosensor analysis and a microscopic observation showed a clear difference on the levels of UPR induction and mis-sorting of secretory protein into vacuoles among the wild-type and mutated MFα signal peptides. This work demonstrates the importance of avoiding entry of secretory proteins into the intracellular protein degradation pathways, an observation that is expected to contribute to the engineering of strains with increased production of recombinant secreted proteins.

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