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Appendix 1
Appendix 1—key resources table
Reagent type
(species) or
resource
Designation
Source or reference
Identifiers
Genetic reagent
(Drosophila
melanogaster)
dlgm52
PMID:9334318
N/A
Genetic reagent
(D. melanogaster) puc-lacZ
PMID:16753569
N/A
Genetic reagent
(D. melanogaster) UAS-Rasv12
PMID:16753569
N/A
Genetic reagent
(D. melanogaster) UAS-BskDN
PMID:10490662
N/A
Genetic reagent
(D. melanogaster) UAS-Src64B
PMID:10069336
N/A
Genetic reagent
(D. melanogaster) Hel25Eccp-8
PMID:31543447
N/A
Genetic reagent
(D. melanogaster) Mahj1
PMID:20644714
N/A
Genetic reagent
(D. melanogaster) UAS-Nact
PMID:15496440
N/A
Genetic reagent
(D. melanogaster) UAS-RNF146
PMID:30796047
N/A
Genetic reagent
(D. melanogaster) lgl4
Bloomington Drosophila
Stock Center
BDSC:36289
Genetic reagent
(D. melanogaster) UAS-p35
Bloomington Drosophila
Stock Center
BDSC:5073
Genetic reagent
(D. melanogaster) UAS-PVRact
Bloomington Drosophila
Stock Center
BDSC:58496
Genetic reagent
(D. melanogaster) UAS-YkiS168A
Bloomington Drosophila
Stock Center
BDSC:28836
Genetic reagent
(D. melanogaster) UAS-Luciferase
Bloomington Drosophila
Stock Center
BDSC:35788
Genetic reagent
(D. melanogaster) UAS-RFP
Bloomington Drosophila
Stock Center
BDSC:30556
Genetic reagent
(D. melanogaster) UAS-bantam
Bloomington Drosophila
Stock Center
BDSC:60672
Genetic reagent
(D. melanogaster) UAS-miR-9c,306,79,9b
Bloomington Drosophila
Stock Center
BDSC:41156
Genetic reagent
(D. melanogaster) UAS-miR-79
Bloomington Drosophila
Stock Center
BDSC:41145
Genetic reagent
UAS-miR-2a-2,2a-
(D. melanogaster) 1,2b-2
Bloomington Drosophila
Stock Center
BDSC:59849
Genetic reagent
(D. melanogaster) UAS-miR-2b-1
Bloomington Drosophila
Stock Center
BDSC:41128
Genetic reagent
(D. melanogaster) UAS-miR-7
Bloomington Drosophila
Stock Center
BDSC:41137
Genetic reagent
(D. melanogaster) UAS-miR-8
Bloomington Drosophila
Stock Center
BDSC:41176
Genetic reagent
(D. melanogaster) UAS-miR-9a
Bloomington Drosophila
Stock Center
BDSC:41138
Genetic reagent
(D. melanogaster) UAS-miR-9b
Bloomington Drosophila
Stock Center
BDSC:41131
Additional information
Appendix 1 Continued on next page
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Appendix 1 Continued
Reagent type
(species) or
resource
Designation
Source or reference
Identifiers
Genetic reagent
(D. melanogaster) UAS-miR-9c
Bloomington Drosophila
Stock Center
BDSC:41139
Genetic reagent
(D. melanogaster) UAS-miR-11
Bloomington Drosophila
Stock Center
BDSC:59865
Genetic reagent
(D. melanogaster) UAS-miR-12
Bloomington Drosophila
Stock Center
BDSC:41140
Genetic reagent
Bloomington Drosophila
(D. melanogaster) UAS-miR-13a,13b-1,2c Stock Center
BDSC:64097
Genetic reagent
(D. melanogaster) UAS-miR-13b-2
Bloomington Drosophila
Stock Center
BDSC:59867
Genetic reagent
(D. melanogaster) UAS-miR-14
Bloomington Drosophila
Stock Center
BDSC:41178
Genetic reagent
(D. melanogaster) UAS-miR-34
Bloomington Drosophila
Stock Center
BDSC:41158
Genetic reagent
(D. melanogaster) UAS-miR-92a
Bloomington Drosophila
Stock Center
BDSC:41153
Genetic reagent
(D. melanogaster) UAS-miR-124
Bloomington Drosophila
Stock Center
BDSC:41126
Genetic reagent
(D. melanogaster) UAS-miR-184
Bloomington Drosophila
Stock Center
BDSC:41174
Genetic reagent
(D. melanogaster) UAS-miR-252
Bloomington Drosophila
Stock Center
BDSC:41127
Genetic reagent
(D. melanogaster) UAS-miR-276a
Bloomington Drosophila
Stock Center
BDSC:41143
Genetic reagent
(D. melanogaster) UAS-miR-276b
Bloomington Drosophila
Stock Center
BDSC:41162
Genetic reagent
(D. melanogaster) UAS-miR-278
Bloomington Drosophila
Stock Center
BDSC:41180
Genetic reagent
(D. melanogaster) UAS-miR-279
Bloomington Drosophila
Stock Center
BDSC:41147
Genetic reagent
(D. melanogaster) UAS-miR-282
Bloomington Drosophila
Stock Center
BDSC:41165
Genetic reagent
(D. melanogaster) UAS-miR-305
Bloomington Drosophila
Stock Center
BDSC:41152
Genetic reagent
(D. melanogaster) UAS-miR-310
Bloomington Drosophila
Stock Center
BDSC:41155
Genetic reagent
(D. melanogaster) UAS-miR-317
Bloomington Drosophila
Stock Center
BDSC:59913
Genetic reagent
(D. melanogaster) UAS-miR-958
Bloomington Drosophila
Stock Center
BDSC:41222
Genetic reagent
(D. melanogaster) UAS-miR-975,976,977
Bloomington Drosophila
Stock Center
BDSC:60635
Genetic reagent
(D. melanogaster) UAS-miR-981
Bloomington Drosophila
Stock Center
BDSC:60639
Genetic reagent
(D. melanogaster) UAS-miR-984
Bloomington Drosophila
Stock Center
BDSC:41224
Genetic reagent
(D. melanogaster) UAS-miR-988
Bloomington Drosophila
Stock Center
BDSC:41196
Genetic reagent
(D. melanogaster) UAS-miR-995
Bloomington Drosophila
Stock Center
BDSC:41199
Additional information
Appendix 1 Continued on next page
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Appendix 1 Continued
Reagent type
(species) or
resource
Designation
Source or reference
Identifiers
Additional information
Genetic reagent
(D. melanogaster) UAS-miR-996
Bloomington Drosophila
Stock Center
BDSC:60653
Genetic reagent
(D. melanogaster) UAS-miR-998
Bloomington Drosophila
Stock Center
BDSC:63043
Genetic reagent
(D. melanogaster) UAS-miR-306-sponge
Bloomington Drosophila
Stock Center
BDSC:61424
Genetic reagent
(D. melanogaster) UAS-miR-79-sponge
Bloomington Drosophila
Stock Center
BDSC:61387
Genetic reagent
(D. melanogaster) UAS-Luciferase RNAi
Bloomington Drosophila
Stock Center
BDSC:31603
Genetic reagent
(D. melanogaster) UAS-aop RNAi
Bloomington Drosophila
Stock Center
BDSC:34909
Genetic reagent
(D. melanogaster) UAS-pde1c RNAi
Bloomington Drosophila
Stock Center
BDSC:55925
Genetic reagent
(D. melanogaster) UAS-atf3 RNAi
Bloomington Drosophila
Stock Center
BDSC:26741
Genetic reagent
(D. melanogaster) UAS-mei-P26 RNAi
Bloomington Drosophila
Stock Center
BDSC:57268
Genetic reagent
(D. melanogaster) UAS-chn RNAi
Bloomington Drosophila
Stock Center
BDSC:26779
Genetic reagent
(D. melanogaster) UAS-chinmo RNAi
Bloomington Drosophila
Stock Center
BDSC:26777
Genetic reagent
(D. melanogaster) UAS-RNF146 RNAi
Bloomington Drosophila
Stock Center
BDSC:40882
Genetic reagent
(D. melanogaster) UAS-bcd RNAi
Bloomington Drosophila
Stock Center
BDSC:33886
Genetic reagent
(D. melanogaster) UAS-CG1358 RNAi
Bloomington Drosophila
Stock Center
BDSC:64848
Genetic reagent
(D. melanogaster) UAS-miR-306
FlyORF
FlyORF: F002214
Genetic reagent
(D. melanogaster) UAS-Tnks
Core Facility of Drosophila
Resource and Technology,
Center for Excellence in
Molecular Cell Science,
Chinese Academy of
Sciences
N/A
Cell line (D.
melanogaster)
S2
ATCC
Antibody
Anti-phospho-JNK
(rabbit monoclonal)
Cell Signaling Technology Cat #4668
1:100
Antibody
Anti-β-galactosidase
(chicken polyclonal)
Abcam
1:1000
Antibody
Anti-cleaved
Drosophila Dcp-1
(Asp216) (rabbit
polyclonal)
Cell Signaling Technology Cat #9578
1:100
Antibody
Goat anti-rabbit
secondary antibody,
Alexa Fluor 647
Thermo Fisher Scientific
Cat #A32733
1:250
Antibody
Goat anti-chicken
secondary antibody,
Alexa Fluor 647
Thermo Fisher Scientific
Cat #A21449
1:250
Cat #CRL-1963
Cat #ab9361
Appendix 1 Continued on next page
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Appendix 1 Continued
Reagent type
(species) or
resource
Designation
Source or reference
Identifiers
Additional information
Antibody
Anti-α-tubulin (mouse
monoclonal)
Sigma-Aldrich
Cat #T5168
1:5000
Antibody
Anti-phospho-JNK
(rabbit polyclonal)
Cell Signaling Technology Cat #9251
1:1000
Antibody
Anti-JNK (mouse
monoclonal)
Santa Cruz Biotechnology Cat #sc-7345
1:1000
Antibody
Anti-RNF146 (rabbit
polyclonal)
GenScript antibody service N/A
Raised in rabbits
against peptide
HSGGGSGEDPAVGSC,1:2000
Antibody
Anti-V5 tag (mouse
monoclonal)
Thermo Fisher Scientific
Cat #R960-25
1:5000
Antibody
Anti-myc tag (rabbit
polyclonal)
MBL
Cat #562
1:1000
Antibody
Horse anti-mouse IgG,
HRP-linked antibody
Cell Signaling Technology Cat #7076
1:5000
Antibody
Goat anti-rabbit IgG,
HRP-linked antibody
1:5000
Cell Signaling Technology Cat #7074
DAPI-containing
Commercial assay SlowFade Gold
or kit
Antifade Reagent
Thermo Fisher Scientific
Cat #S36937
Commercial assay FuGene HD
or kit
transfection reagent
Promega
Cat #PRE2311
Other
Santa Cruz Biotechnology Cat #SC-3508
CHX
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50 μg/ml
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