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FIGURE LEGENDS
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Figure 1. Coculture-induced release of exosomes and VEGF. Coculture of peripheral
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blood eosinophils and NPFs for 24 h stimulated the release of exosomes (A) and VEGF (B)
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(n=4). Coculture of EoL-1 cells and NPFs for 24 h stimulated the release of exosomes (C)
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and VEGF (D) (n=6). *P<0.05 when compared between coculture group and single cell
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group, one-way ANOVA with Tukey's honestly significant difference test or Games-
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Howell test.
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Figure 2. Release of exosomes (A) and VEGF (B) over time in coculture of EoL-1
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cells and NPFs for 3, 6, 12, and 24 h. Coculture-induced release of exosomes and VEGF
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was stimulated at 3 h of incubation. Coculture-induced exosome release continued to
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decrease for up to 24 h of incubation, although coculture-induced VEGF release continued
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to increase for up to 24 h of incubation (n=6). *P<0.05 when compared between coculture
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group and EoL-1 cell group, one-way ANOVA with Tukey's honestly significant difference
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test or Games-Howell test.
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Figure 3. Effect of isolated NPF-derived EVs on the release of VEGF from EoL-1
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cells. (A) Size and concentration of NPF-derived EVs as determined by nanoparticle
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tracking analysis. (B) Transmission electron microscopy of NPF-derived EVs. (C) Isolated
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NPF-derived EVs (exosome concentration: 25 pg/mL) significantly stimulated VEGF
434
release from EoL-1 cells (n=4). *P<0.05, unpaired t-test.
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Figure 4. Effects of GW4869 or DMA on coculture-induced release of exosomes and
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VEGF. Pretreatment of NPFs for 18 h with GW4869 (10 μM), an inhibitor of exosome
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production, significantly attenuated the release of exosomes (A) and VEGF (B) from
21
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cocultured EoL-1 cells and NPFs at 24 h (n=6). Pretreatment of NPFs for 18 h with DMA
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(10 μg/mL), an inhibitor of exosome secretion, significantly attenuated the release of
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exosomes (C) at 6 and 24 h and VEGF (D) at 3, 6, and 24 h from cocultured EoL-1 cells
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and NPFs at 3, 6, and 24 h (n=4). *P<0.05, unpaired t-test.
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