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Acknowledgements
This work was financially supported by Kikkoman Corporation and JST, COI-NEXT (grant number JPMJPF2008), Japan.
Author contributions
K.K. conceived the project, and R.T. and K.K. designed the experiments. Y.T. conducted the experiments using
nitrogenase-producing Escherichia coli. R.T. conducted all other experiments. W.A. supported the operation of
the LC–MS system and data acquisition. Y.K. provided instructions regarding the preparation of protein digests
and assisted with the related experiments. H.M. prepared the long monolithic column. R.T. and K.K. interpreted
the results. K.K. and M.U. supervised the project. R.T. and K.K. wrote the manuscript in consultation with M.U.
Competing interests Kyoto Monotech provided a monolithic column and supports H.M. in the form of salaries. R.T., Y. T., W. A., Y.
K., M. U., and K. K. all declare no conflict of interest.
Additional information
Supplementary Information The online version contains supplementary material available at https://doi.org/
10.1038/s41598-022-08007-4.
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